PMID- 30052442
OWN - NLM
STAT- MEDLINE
DCOM- 20180820
LR  - 20180820
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 66
IP  - 32
DP  - 2018 Aug 15
TI  - Identification and Characterization of beta-Lathyrin, an Abundant Glycoprotein of 
      Grass Pea ( Lathyrus sativus L.), as a Potential Allergen.
PG  - 8496-8503
LID - 10.1021/acs.jafc.8b02314 [doi]
AB  - Grass pea, a protein-rich, high-yielding, and drought-tolerant legume, is used as 
      food and livestock feed in several tropical and subtropical regions of the world. 
      The abundant seed proteins of grass pea are salt-soluble globulins, which can be 
      separated into vicilins and legumins. In many other legumes, the members of 
      vicilin seed proteins have been identified as major allergens. However, very 
      little information is available on the allergens of grass pea. In this study, we 
      have identified an abundant 47 kDa protein from grass pea, which was recognized 
      by immunoglobulin E (IgE) antibodies from sera drawn from several peanut-allergic 
      patients. The IgE-binding 47 kDa protein was partially purified by affinity 
      chromatography on a Con-A sepharose column. Matrix-assisted laser 
      desorption/ionization-time-of-flight mass spectrometry analysis of the 47 kDa 
      grass pea protein revealed sequence homology to 47 kDa vicilin from pea and Len c 
      1 from lentil. Interestingly the grass pea vicilin was found to be susceptible to 
      pepsin digestion in vitro. We have also isolated a cDNA encoding the grass pea 47 
      kDa vicilin (beta-lathyrin), and the deduced amino acid sequence revealed extensive 
      homology to several known allergens, including those from peanut and soybean. A 
      homology model structure of the grass pea beta-lathyrin, generated using the X-ray 
      crystal structure of the soybean beta-conglycinin beta subunit as a template, revealed 
      potential IgE-binding epitopes located on the surface of the molecule. The 
      similarity in the three-dimensional structure and the conservation of the 
      antigenic epitopes on the molecular surface of vicilin allergens explains the 
      IgE-binding cross-reactivity.
FAU - Xu, Quanle
AU  - Xu Q
AD  - College of Life Sciences , Northwest A&F University , Yangling , Shaanxi 712100 , 
      People's Republic of China.
AD  - Plant Science Division , University of Missouri , Columbia , Missouri 65211 , 
      United States.
FAU - Song, Bo
AU  - Song B
AD  - Plant Science Division , University of Missouri , Columbia , Missouri 65211 , 
      United States.
AD  - Key Laboratory of Soybean Biology at the Chinese Ministry of Education , 
      Northeast Agricultural University , Harbin , Heilongjiang 150030 , People's 
      Republic of China.
FAU - Liu, Fengjuan
AU  - Liu F
AD  - College of Life Sciences , Northwest A&F University , Yangling , Shaanxi 712100 , 
      People's Republic of China.
FAU - Song, Yaoyao
AU  - Song Y
AD  - College of Life Sciences , Northwest A&F University , Yangling , Shaanxi 712100 , 
      People's Republic of China.
FAU - Chen, Peng
AU  - Chen P
AD  - College of Life Sciences , Northwest A&F University , Yangling , Shaanxi 712100 , 
      People's Republic of China.
FAU - Liu, Shanshan
AU  - Liu S
AD  - Key Laboratory of Soybean Biology at the Chinese Ministry of Education , 
      Northeast Agricultural University , Harbin , Heilongjiang 150030 , People's 
      Republic of China.
FAU - Krishnan, Hari B
AU  - Krishnan HB
AUID- ORCID: 0000-0001-6437-3672
AD  - Plant Science Division , University of Missouri , Columbia , Missouri 65211 , 
      United States.
AD  - Plant Genetics Research, Agricultural Research Service (ARS) , United States 
      Department of Agriculture (USDA) , Columbia , Missouri 65211 , United States.
LA  - eng
PT  - Journal Article
DEP - 20180807
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Allergens)
RN  - 0 (Glycoproteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Seed Storage Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9067-60-1 (vicilin protein, plant)
SB  - IM
MH  - Allergens/*chemistry/immunology
MH  - Arachis/genetics/immunology
MH  - Food Hypersensitivity/immunology
MH  - Glycoproteins/*chemistry/immunology
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Lathyrus/*chemistry
MH  - Lens Plant/chemistry/immunology
MH  - Mass Spectrometry
MH  - Plant Proteins/*chemistry/immunology
MH  - Seed Storage Proteins/chemistry/immunology
OTO - NOTNLM
OT  - allergen
OT  - grass pea
OT  - storage protein
OT  - beta-lathyrin
EDAT- 2018/07/28 06:00
MHDA- 2018/08/21 06:00
CRDT- 2018/07/28 06:00
PHST- 2018/07/28 06:00 [pubmed]
PHST- 2018/08/21 06:00 [medline]
PHST- 2018/07/28 06:00 [entrez]
AID - 10.1021/acs.jafc.8b02314 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2018 Aug 15;66(32):8496-8503. doi: 10.1021/acs.jafc.8b02314. 
      Epub 2018 Aug 7.