PMID- 30053709
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 159
DP  - 2018 Sep 10
TI  - Determination of garlic phenolic compounds using supercritical fluid extraction 
      coupled to supercritical fluid chromatography/tandem mass spectrometry.
PG  - 513-523
LID - S0731-7085(18)31281-0 [pii]
LID - 10.1016/j.jpba.2018.07.020 [doi]
AB  - A rapid, simple and environmentally friendly supercritical fluid extraction and 
      supercritical fluid chromatography coupled to mass spectrometry (SFE-SFC-MS/MS) 
      method has been developed for the analysis of nine phenolic compounds in garlic. 
      Through the optimization of the SFE parameters using response surface methodology 
      (RSM), 15 phenolic compounds were successfully extracted at 50  degrees C in 9 min with 
      the addition of 30% methanol using a method that was automatic, efficient, green 
      and prevented oxidation. Moreover, the operating conditions of the SFC-MS/MS were 
      also optimized for the analysis of the SFE extracts. 15 phenolic compounds in the 
      garlic showed good separation performance on a Shim-pack UC-X Diol column, and 
      0.1 mM oxalic acid and 1 mM ammonium formate in methanol were chosen as the most 
      suitable mobile phase components. Furthermore, a calibration curve was developed 
      using a matrix-matched standard, and 9 phenolic compounds showed good linearity 
      (R(2) > 0.99). The limits of detection of the 9 phenolic compounds varied from 
      0.6 to 12 ng/g, and the quantifications ranged from 2 to 40 ng/g. The validation 
      data also showed acceptable recoveries for 9 phenolic compounds, and the relative 
      standard deviations (RSDs) for the overall precision were lower than 10%. The 
      developed method has been utilized to analyze the phenolic compounds in garlic 
      from different geographical areas of China (Cangshan, Jinxiang, Pizhou, Dali), 
      which suggests that this off-line SFE-SFC-MS/MS method could be adapted to the 
      quantitative analysis of phenolic compounds in garlic.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Liu, Jian
AU  - Liu J
AD  - Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 
      100176, China; School of Food Science and Engineering, Hefei University of 
      Technology, Hefei 230009, China.
FAU - Ji, Feng
AU  - Ji F
AD  - Shimadzu (China) Co., LTD., Beijing, 100020, China.
FAU - Chen, Fengming
AU  - Chen F
AD  - Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 
      100176, China.
FAU - Guo, Wei
AU  - Guo W
AD  - Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 
      100176, China.
FAU - Yang, Minli
AU  - Yang M
AD  - Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 
      100176, China.
FAU - Huang, Shengxiong
AU  - Huang S
AD  - School of Food Science and Engineering, Hefei University of Technology, Hefei 
      230009, China.
FAU - Zhang, Feng
AU  - Zhang F
AD  - Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 
      100176, China. Electronic address: fengzhang@126.com.
FAU - Liu, Yongsheng
AU  - Liu Y
AD  - School of Food Science and Engineering, Hefei University of Technology, Hefei 
      230009, China. Electronic address: liuyongsheng1122@hfut.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180720
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Phenols)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Chromatography, Supercritical Fluid/*methods
MH  - Garlic/*chemistry
MH  - Limit of Detection
MH  - Phenols/*analysis
MH  - Plant Extracts/chemistry
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Garlic
OT  - Phenolic compounds
OT  - Supercritical fluid chromatography
OT  - Supercritical fluid extraction
EDAT- 2018/07/28 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/07/28 06:00
PHST- 2018/05/29 00:00 [received]
PHST- 2018/07/14 00:00 [revised]
PHST- 2018/07/15 00:00 [accepted]
PHST- 2018/07/28 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/07/28 06:00 [entrez]
AID - S0731-7085(18)31281-0 [pii]
AID - 10.1016/j.jpba.2018.07.020 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2018 Sep 10;159:513-523. doi: 10.1016/j.jpba.2018.07.020. 
      Epub 2018 Jul 20.