PMID- 30055802
OWN - NLM
STAT- MEDLINE
DCOM- 20190312
LR  - 20190312
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 503
IP  - 3
DP  - 2018 Sep 10
TI  - Dihydromyricetin inhibits caerulin-induced TRAF3-p38 signaling activation and 
      acute pancreatitis response.
PG  - 1696-1702
LID - S0006-291X(18)31603-6 [pii]
LID - 10.1016/j.bbrc.2018.07.101 [doi]
AB  - Acute pancreatitis (AP) is a common inflammatory disease in gastrointestinal 
      tract. Our previous study has shown that caerulin induces TNF receptor-associated 
      factor 3 (TRAF3)-p38 signaling activation and pro-inflammatory response in 
      macrophages, causing damage to co-cultured pancreatic acinar cells. 
      Dihydromyricetin (DHM) is a flavonoid extracted from Ampelopsis grossedentata, 
      which has displayed anti-inflammation and anti-oxidant functions. Our results 
      here show that DHM potently inhibited caerulin-induced expression and productions 
      of multiple pro-inflammatory cytokines (IL-1beta, TNF-alpha and IL-17) in murine bone 
      marrow-derived macrophages (BMDMs). DHM significantly inhibited caerulin-induced 
      TRAF3 protein stabilization, TRAF3-mitogen-activated protein kinase kinase 3 
      (MKK3) association and following MKK3-p38 activation in BMDMs. Significantly, DHM 
      was ineffective against caerulin in TRAF3-silenced BMDMs. Importantly, DHM 
      supplement attenuated the cytotoxicity of caerulin-activated BMDMs to co-cultured 
      pancreatic acinar cells, resulting in significantly decreased acinar cell death 
      and apoptosis. In vivo, DHM co-administration largely attenuated pancreatic and 
      systemic inflammation in caerulin-injected AP mice. Together, DHM inhibits 
      caerulin-induced TRAF3-p38 signaling activation and AP response. DHM could be 
      further studied as a potential anti-AP agent.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Jia, Rongrong
AU  - Jia R
AD  - Department of Gastroenterology, Shanghai Tongren Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Ma, Jiali
AU  - Ma J
AD  - Department of Gastroenterology, Shanghai Tongren Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Meng, Wenying
AU  - Meng W
AD  - Department of Gastroenterology, Shanghai Tongren Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China.
FAU - Wang, Na
AU  - Wang N
AD  - Department of Gastroenterology, Shanghai Tongren Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, China. Electronic address: 
      wangnatongren@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180726
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Cytokines)
RN  - 0 (Flavonols)
RN  - 0 (TNF Receptor-Associated Factor 3)
RN  - 888Y08971B (Ceruletide)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - KD8QND6427 (dihydromyricetin)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Cell Death/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Ceruletide/*antagonists & inhibitors/pharmacology
MH  - Cytokines/antagonists & inhibitors/biosynthesis
MH  - Flavonols/*pharmacology
MH  - Macrophages/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pancreatitis/*drug therapy/metabolism/pathology
MH  - Signal Transduction/*drug effects
MH  - TNF Receptor-Associated Factor 3/*metabolism
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
OTO - NOTNLM
OT  - Acute pancreatitis
OT  - Caerulin
OT  - Dihydromyricetin
OT  - Pro-inflammatory response
OT  - TRAF3
EDAT- 2018/07/30 06:00
MHDA- 2019/03/13 06:00
CRDT- 2018/07/30 06:00
PHST- 2018/07/17 00:00 [received]
PHST- 2018/07/20 00:00 [accepted]
PHST- 2018/07/30 06:00 [pubmed]
PHST- 2019/03/13 06:00 [medline]
PHST- 2018/07/30 06:00 [entrez]
AID - S0006-291X(18)31603-6 [pii]
AID - 10.1016/j.bbrc.2018.07.101 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Sep 10;503(3):1696-1702. doi: 
      10.1016/j.bbrc.2018.07.101. Epub 2018 Jul 26.