PMID- 30057317
OWN - NLM
STAT- MEDLINE
DCOM- 20190315
LR  - 20190315
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 503
IP  - 3
DP  - 2018 Sep 10
TI  - St6gal1 knockdown alters HBV life cycle in HepAD38 cells.
PG  - 1841-1847
LID - S0006-291X(18)31626-7 [pii]
LID - 10.1016/j.bbrc.2018.07.124 [doi]
AB  - Complex glycans at the cell surface play important roles, and their alteration is 
      known to modulate cellular activity. Previously, we found that HBV replication in 
      HepAD38 altered cell-surface sialylated N-glycan through the upregulation of 
      St6gal1, Mgat2, and Mgat4a expression. Here we studied the effects of knocking 
      them down on HBV replication in HepAD38. Our results showed that St6gal1 
      knockdown (KD) reduced intracellular HBV rcDNA level by 90%, that Mgat2 KD did 
      not change the intracellular HBV rcDNA level, and that Mgat4 KD increased the 
      intracellular HBV rcDNA level by 19 times compared to Tet(-). The changes in 
      intracellular rcDNA level were followed by the alteration of Pol and HBc 
      expression. Our study suggests that St6gal1 KD contributes more to the HBV life 
      cycle than Mgat2 or Mgat4a KD through the modification of intracellular L, Pol, 
      and HBc expression.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Priyambada, Surya Agung
AU  - Priyambada SA
AD  - International Center for Biotechnology, Osaka University, Yamada-oka 2-1, Suita, 
      Osaka, 565-0871, Japan.
FAU - Misaki, Ryo
AU  - Misaki R
AD  - International Center for Biotechnology, Osaka University, Yamada-oka 2-1, Suita, 
      Osaka, 565-0871, Japan.
FAU - Okamoto, Toru
AU  - Okamoto T
AD  - Department of Molecular Virology, Research Institute for Microbial Disease, Osaka 
      University, Yamada-oka 3-1, Suita, Osaka, 565-0871, Japan.
FAU - Ohashi, Takao
AU  - Ohashi T
AD  - International Center for Biotechnology, Osaka University, Yamada-oka 2-1, Suita, 
      Osaka, 565-0871, Japan.
FAU - Ueda, Keiji
AU  - Ueda K
AD  - Division of Virology, Department of Microbiology and Immunology, Graduate School 
      of Medicine, Osaka University, Yamada-oka 2-2, Suita, Osaka, 565-0871, Japan.
FAU - Matsuura, Yoshiharu
AU  - Matsuura Y
AD  - Department of Molecular Virology, Research Institute for Microbial Disease, Osaka 
      University, Yamada-oka 3-1, Suita, Osaka, 565-0871, Japan.
FAU - Fujiyama, Kazuhito
AU  - Fujiyama K
AD  - International Center for Biotechnology, Osaka University, Yamada-oka 2-1, Suita, 
      Osaka, 565-0871, Japan. Electronic address: fujiyama@icb.osaka-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180726
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, CD)
RN  - 0 (DNA, Circular)
RN  - EC 2.4.- (Glycosyltransferases)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - EC 2.4.99.1 (ST6GAL1 protein, human)
SB  - IM
MH  - Antigens, CD/genetics/metabolism
MH  - DNA, Circular/genetics/metabolism
MH  - Glycosyltransferases/metabolism
MH  - Hepatitis B virus/*growth & development
MH  - Humans
MH  - Mutation
MH  - Sialyltransferases/*deficiency/genetics/metabolism
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - Complex-type N-glycan
OT  - Hepatitis B virus
OT  - N-glycan
OT  - Sialylated N-glycan
EDAT- 2018/07/31 06:00
MHDA- 2019/03/16 06:00
CRDT- 2018/07/31 06:00
PHST- 2018/07/19 00:00 [received]
PHST- 2018/07/24 00:00 [accepted]
PHST- 2018/07/31 06:00 [pubmed]
PHST- 2019/03/16 06:00 [medline]
PHST- 2018/07/31 06:00 [entrez]
AID - S0006-291X(18)31626-7 [pii]
AID - 10.1016/j.bbrc.2018.07.124 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Sep 10;503(3):1841-1847. doi: 
      10.1016/j.bbrc.2018.07.124. Epub 2018 Jul 26.