PMID- 30058456
OWN - NLM
STAT- MEDLINE
DCOM- 20190802
LR  - 20240402
IS  - 1559-7016 (Electronic)
IS  - 0271-678X (Print)
IS  - 0271-678X (Linking)
VI  - 38
IP  - 10
DP  - 2018 Oct
TI  - In-vitro blood-brain barrier modeling: A review of modern and fast-advancing 
      technologies.
PG  - 1667-1681
LID - 10.1177/0271678X18788769 [doi]
AB  - The development of realistic in vitro blood-brain barrier (BBB) models that 
      recapitulate the physiological parameters and molecular aspect of the 
      neurovascular unit (NVU) is of fundamental importance not only in CNS drug 
      discovery but also in translational research. Successful modeling of the NVU 
      would provide an invaluable tool to aid in dissecting out the pathological 
      factors, mechanism of action (and corresponding targets) prodromal to the onset 
      of CNS disorders. The field of BBB in vitro modeling has seen many radical 
      changes in the last few years with the introduction on novel technologies and 
      methods to improve over existing models and develop new ones. Therefore, the goal 
      of this review is to provide the readers with updated technical and operational 
      details concerning current BBB platforms with special focus on stem cell 
      technology used to establish a functional BBB model in vitro. Furthermore, we 
      provide a detailed update on rapidly advancing 3D printing technologies used for 
      engineering BBB models which use is now fast expanding among researchers.
FAU - Sivandzade, Farzane
AU  - Sivandzade F
AD  - 1 Department of Pharmaceutical Sciences, Texas Tech University Health Sciences 
      Center, Amarillo, TX, USA.
FAU - Cucullo, Luca
AU  - Cucullo L
AD  - 1 Department of Pharmaceutical Sciences, Texas Tech University Health Sciences 
      Center, Amarillo, TX, USA.
AD  - 2 Center for Blood Brain Barrier Research, Texas Tech University Health Sciences 
      Center, Amarillo, TX, USA.
LA  - eng
GR  - R01 DA029121/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20180730
PL  - United States
TA  - J Cereb Blood Flow Metab
JT  - Journal of cerebral blood flow and metabolism : official journal of the 
      International Society of Cerebral Blood Flow and Metabolism
JID - 8112566
MH  - *Blood-Brain Barrier
MH  - Humans
MH  - In Vitro Techniques/*methods/trends
MH  - *Models, Biological
PMC - PMC6168917
OTO - NOTNLM
OT  - Blood-brain barrier
OT  - alternative
OT  - bio-printing
OT  - cell line
OT  - drug development
OT  - drug discovery
OT  - endothelium
OT  - microfluidic
OT  - stem cells cell lines
EDAT- 2018/07/31 06:00
MHDA- 2019/08/03 06:00
PMCR- 2019/10/01
CRDT- 2018/07/31 06:00
PHST- 2018/07/31 06:00 [pubmed]
PHST- 2019/08/03 06:00 [medline]
PHST- 2018/07/31 06:00 [entrez]
PHST- 2019/10/01 00:00 [pmc-release]
AID - 10.1177_0271678X18788769 [pii]
AID - 10.1177/0271678X18788769 [doi]
PST - ppublish
SO  - J Cereb Blood Flow Metab. 2018 Oct;38(10):1667-1681. doi: 
      10.1177/0271678X18788769. Epub 2018 Jul 30.