PMID- 30059007
OWN - NLM
STAT- MEDLINE
DCOM- 20190226
LR  - 20190524
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 7
DP  - 2018 Jul 30
TI  - Differential expression of Lutheran/BCAM regulates biliary tissue remodeling in 
      ductular reaction during liver regeneration.
LID - 10.7554/eLife.36572 [doi]
LID - e36572
AB  - Under chronic or severe liver injury, liver progenitor cells (LPCs) of biliary 
      origin are known to expand and contribute to the regeneration of hepatocytes and 
      cholangiocytes. This regeneration process is called ductular reaction (DR), which 
      is accompanied by dynamic remodeling of biliary tissue. Although the DR shows 
      apparently distinct mode of biliary extension depending on the type of liver 
      injury, the key regulatory mechanism remains poorly understood. Here, we show 
      that Lutheran (Lu)/Basal cell adhesion molecule (BCAM) regulates the 
      morphogenesis of DR depending on liver disease models. Lu(+) and Lu(-) biliary 
      cells isolated from injured liver exhibit opposite phenotypes in cell motility 
      and duct formation capacities in vitro. By overexpression of Lu, Lu(-) biliary 
      cells acquire the phenotype of Lu(+) biliary cells. Lu-deficient mice showed 
      severe defects in DR. Our findings reveal a critical role of Lu in the control of 
      phenotypic heterogeneity of DR in distinct liver disease models.
CI  - (c) 2018, Miura et al.
FAU - Miura, Yasushi
AU  - Miura Y
AD  - Department of Regenerative Medicine, Research Institute, National Center for 
      Global Health and Medicine, Tokyo, Japan.
AD  - Department of Life Science and Medical Bioscience, Graduate School of Advanced 
      Science and Engineering, Waseda University, Tokyo, Japan.
FAU - Matsui, Satoshi
AU  - Matsui S
AD  - Department of Regenerative Medicine, Research Institute, National Center for 
      Global Health and Medicine, Tokyo, Japan.
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
FAU - Miyata, Naoko
AU  - Miyata N
AD  - Department of Regenerative Medicine, Research Institute, National Center for 
      Global Health and Medicine, Tokyo, Japan.
FAU - Harada, Kenichi
AU  - Harada K
AD  - Department of Human Pathology, Kanazawa University Graduate School of Medicine, 
      Kanazawa, Japan.
FAU - Kikkawa, Yamato
AU  - Kikkawa Y
AD  - Department of Clinical Biochemistry, Tokyo University of Pharmacy and Life 
      Sciences, Tokyo, Japan.
FAU - Ohmuraya, Masaki
AU  - Ohmuraya M
AD  - Department of Genetics, Hyogo College of Medicine, Hyogo, Japan.
FAU - Araki, Kimi
AU  - Araki K
AD  - Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, 
      Japan.
FAU - Tsurusaki, Shinya
AU  - Tsurusaki S
AD  - Department of Regenerative Medicine, Research Institute, National Center for 
      Global Health and Medicine, Tokyo, Japan.
AD  - Laboratory of Stem Cell Regulation, Institute of Molecular and Cellular 
      Biosciences, The University of Tokyo, Tokyo, Japan.
FAU - Okochi, Hitoshi
AU  - Okochi H
AD  - Department of Regenerative Medicine, Research Institute, National Center for 
      Global Health and Medicine, Tokyo, Japan.
FAU - Goda, Nobuhito
AU  - Goda N
AD  - Department of Life Science and Medical Bioscience, Graduate School of Advanced 
      Science and Engineering, Waseda University, Tokyo, Japan.
FAU - Miyajima, Atsushi
AU  - Miyajima A
AD  - Laboratory of Cell Growth and Differentiation, Institute of Molecular and 
      Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
FAU - Tanaka, Minoru
AU  - Tanaka M
AUID- ORCID: 0000-0003-2500-7973
AD  - Department of Regenerative Medicine, Research Institute, National Center for 
      Global Health and Medicine, Tokyo, Japan.
AD  - Laboratory of Stem Cell Regulation, Institute of Molecular and Cellular 
      Biosciences, The University of Tokyo, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180730
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
RN  - 0 (BCAM protein, human)
RN  - 0 (Bcam protein, mouse)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Epithelial Cell Adhesion Molecule)
RN  - 0 (Integrin beta1)
RN  - 0 (Laminin)
RN  - 0 (Lutheran Blood-Group System)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (laminin alpha5)
RN  - N91BDP6H0X (Choline)
SB  - IM
MH  - Animals
MH  - Antibody Specificity/immunology
MH  - Bile Ducts/*metabolism/*physiology
MH  - Cell Adhesion Molecules/*metabolism
MH  - Cell Movement/genetics
MH  - Cell Separation
MH  - Choline
MH  - Diet
MH  - Disease Models, Animal
MH  - Epithelial Cell Adhesion Molecule/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Integrin beta1/genetics/metabolism
MH  - Laminin/metabolism
MH  - Liver/metabolism
MH  - *Liver Regeneration/genetics
MH  - Lutheran Blood-Group System/*metabolism
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Models, Biological
MH  - Phenotype
MH  - RNA, Messenger/genetics/metabolism
MH  - Reproducibility of Results
PMC - PMC6107333
OTO - NOTNLM
OT  - developmental biology
OT  - heterogeneity
OT  - mouse
OT  - progenitor
OT  - regeneration
OT  - regenerative medicine
OT  - remodeling
OT  - stem cells
COIS- YM, SM, NM, KH, YK, MO, KA, ST, HO, NG, AM, MT No competing interests declared
EDAT- 2018/07/31 06:00
MHDA- 2019/02/27 06:00
PMCR- 2018/07/30
CRDT- 2018/07/31 06:00
PHST- 2018/03/11 00:00 [received]
PHST- 2018/07/28 00:00 [accepted]
PHST- 2018/07/31 06:00 [pubmed]
PHST- 2019/02/27 06:00 [medline]
PHST- 2018/07/31 06:00 [entrez]
PHST- 2018/07/30 00:00 [pmc-release]
AID - 36572 [pii]
AID - 10.7554/eLife.36572 [doi]
PST - epublish
SO  - Elife. 2018 Jul 30;7:e36572. doi: 10.7554/eLife.36572.