PMID- 30062787 OWN - NLM STAT- MEDLINE DCOM- 20190529 LR - 20200225 IS - 1469-445X (Electronic) IS - 0958-0670 (Print) IS - 0958-0670 (Linking) VI - 103 IP - 11 DP - 2018 Nov TI - Type 2 diabetes and older age contribute to elevated plasma microparticle concentrations independent of chronic stroke. PG - 1560-1570 LID - 10.1113/EP087116 [doi] AB - NEW FINDINGS: What is the central question of this study? What is the effect of chronic stroke on circulating microparticle populations, accounting for potential effects of age and type 2 diabetes? What is the main finding and its importance? Elevated concentrations of CD31(+) /CD42b(-) and CD62E(+) microparticles appear to be driven by type 2 diabetes but not chronic stroke and are associated with fasting glucose and triglyceride levels. Older age results in elevations in CD62E(+) and CD34(+) microparticle concentrations. These microparticles have been proposed as potential targets for diagnosing, treating and identifying the clinical progression and complications of type 2 diabetes. ABSTRACT: The elevated circulating concentration of endothelial microparticles (MPs) may provide an index of the extent and nature of cellular damage in chronic stroke. The purpose of this study was to determine the circulating concentrations of CD31(+) /CD42b(-) , CD62E(+) and CD34(+) MPs in chronic stroke subjects, focusing on the effects of chronic stroke by comparison with both older adults without a history of stroke but with type 2 diabetes mellitus (T2DM) and older and young healthy controls. Plasma from three groups of sedentary older (50-75 years) men and women (chronic stroke, T2DM or older healthy) as well as a group of younger (18-39 years) healthy controls was isolated from fasting blood, and CD31(+) /CD42b(-) , CD62E(+) and CD34(+) MPs were quantified using flow cytometry (n = 17/group). Concentrations of CD31(+) /CD42b(-) and CD62E(+) MPs were higher in the T2DM group (P < 0.05), but not chronic stroke, compared to older and younger healthy adults. CD62E(+) MP and CD34(+) MP concentrations were elevated in the older compared to younger adults (P < 0.05 for both). Sub-analyses excluding chronic stroke subjects who were also diagnosed with diabetes [stroke (diabetes(-) )] revealed lower CD31(+) /CD42b(-) (P < 0.05) and CD62E(+) (P = 0.08) MPs in the stroke (diabetes(-) ) group compared to the T2DM group. CD31(+) /CD42b(-) MP and CD62E(+) MP concentrations were each associated with fasting glucose levels and CD31(+) /CD42b(-) MPs also were associated with triglyceride levels. As MPs have been proposed as potential targets for diagnosing, treating and identifying the clinical progression of T2DM, our study provides further support for the use of CD31(+) /CD42b(-) and CD62E(+) MPs in the clinical progression of T2DM and associated vascular complications. CI - (c) 2018 The Authors. Experimental Physiology (c) 2018 The Physiological Society. FAU - Landers-Ramos, Rian Q AU - Landers-Ramos RQ AD - Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA. AD - Baltimore Veterans Affairs Geriatric Research, Education and Clinical Center, Baltimor, MD, USA. AD - Department of Kinesiology, University of Maryland, College Park, MD, USA. FAU - Serra, Monica C AU - Serra MC AD - Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA. AD - Baltimore Veterans Affairs Geriatric Research, Education and Clinical Center, Baltimor, MD, USA. AD - Emory University School of Medicine and Atlanta Veterans Affairs Medical Center, Atlanta, GA, USA. FAU - Blumenthal, Jacob B AU - Blumenthal JB AD - Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA. AD - Baltimore Veterans Affairs Geriatric Research, Education and Clinical Center, Baltimor, MD, USA. FAU - Ryan, Alice S AU - Ryan AS AD - Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA. AD - Baltimore Veterans Affairs Geriatric Research, Education and Clinical Center, Baltimor, MD, USA. FAU - Hafer-Macko, Charlene E AU - Hafer-Macko CE AD - Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA. AD - Baltimore Veterans Affairs Geriatric Research, Education and Clinical Center, Baltimor, MD, USA. FAU - Prior, Steven J AU - Prior SJ AD - Division of Gerontology and Geriatric Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA. AD - Baltimore Veterans Affairs Geriatric Research, Education and Clinical Center, Baltimor, MD, USA. AD - Department of Kinesiology, University of Maryland, College Park, MD, USA. LA - eng GR - T32 HL007698/HL/NHLBI NIH HHS/United States GR - R01 AG030075/AG/NIA NIH HHS/United States GR - I01 CX000730/CX/CSRD VA/United States GR - P30 AG028747/AG/NIA NIH HHS/United States GR - K23 AG040775/AG/NIA NIH HHS/United States GR - IK2 RX000944/RX/RRD VA/United States GR - R01-AG030075/NH/NIH HHS/United States GR - K23-AG040775/NH/NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20180902 PL - England TA - Exp Physiol JT - Experimental physiology JID - 9002940 SB - IM MH - Adolescent MH - Adult MH - Age Factors MH - Aged MH - Cell-Derived Microparticles MH - Diabetes Mellitus, Type 2/*blood/complications MH - Female MH - Humans MH - Male MH - Middle Aged MH - Oxygen Consumption/physiology MH - Risk Factors MH - Stroke/*blood/complications MH - Young Adult PMC - PMC6449859 MID - NIHMS983741 OTO - NOTNLM OT - diabetes mellitus OT - endothelial microparticle OT - older age OT - stroke COIS- DISCLOSURES No conflicts of interest, financial or otherwise, are declared by the author(s). EDAT- 2018/08/01 06:00 MHDA- 2019/05/30 06:00 PMCR- 2019/11/01 CRDT- 2018/08/01 06:00 PHST- 2018/05/15 00:00 [received] PHST- 2018/07/30 00:00 [accepted] PHST- 2018/08/01 06:00 [pubmed] PHST- 2019/05/30 06:00 [medline] PHST- 2018/08/01 06:00 [entrez] PHST- 2019/11/01 00:00 [pmc-release] AID - 10.1113/EP087116 [doi] PST - ppublish SO - Exp Physiol. 2018 Nov;103(11):1560-1570. doi: 10.1113/EP087116. Epub 2018 Sep 2.