PMID- 30064017
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 81
DP  - 2018 Oct
TI  - A single C4 Zinc finger-containing protein from Litopenaeus vannamei involved in 
      antibacterial responses.
PG  - 493-501
LID - S1050-4648(18)30461-3 [pii]
LID - 10.1016/j.fsi.2018.07.053 [doi]
AB  - The Zinc finger domains (ZnFs), which contain finger-like protrusions stabilized 
      by zinc ions and function to bind DNA, RNA, protein and lipid substrates, are 
      ubiquitously present in a large number of proteins. In this study, a novel 
      protein containing a single C4 type Znf domain (SZnf) was identified from Pacific 
      white shrimp, Litopenaeus vannamei and its role in immunity was further 
      investigated. The ZnF domain of SZnF but not other regions shared high homology 
      with those of fushi tarazu-factor 1 (FTZ-F1) proteins. The SZnF protein was 
      mainly localized in the cytoplasm and was also present in the nucleus at a small 
      level. SZnF was high expressed in the scape and muscle tissues of healthy shrimp 
      and its expression in gill and heptopancreas was strongly up-regulated during 
      bacterial infection. Silencing of SZnf in vivo could strongly increase the 
      susceptibility of shrimp to infection with Vibrio parahaemolyticus but not white 
      spot syndrome virus (WSSV), suggesting that SZnf could be mainly involved in 
      antibacterial responses. Both dual luciferase reporter assays and real-time PCR 
      analysis demonstrated that SZnf could positively regulate the expression of 
      various antimicrobial peptides in vitro and in vivo, which could be part of the 
      mechanism underlying its antibacterial effects. In summary, the current study 
      could help learn more about the function of ZnF-containing proteins and the 
      regulatory mechanisms of immune responses against pathogen infection in 
      crustaceans.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Zuo, Hongliang
AU  - Zuo H
AD  - MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, 
      School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute 
      of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic 
      Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
FAU - Yang, Linwei
AU  - Yang L
AD  - MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, 
      School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute 
      of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic 
      Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
FAU - Zheng, Jiefu
AU  - Zheng J
AD  - MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, 
      School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute 
      of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic 
      Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
FAU - Su, Ziqi
AU  - Su Z
AD  - MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, 
      School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute 
      of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic 
      Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
FAU - Weng, Shaoping
AU  - Weng S
AD  - MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, 
      School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute 
      of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic 
      Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
FAU - He, Jianguo
AU  - He J
AD  - MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, 
      School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute 
      of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic 
      Economic Animals, Sun Yat-sen University, Guangzhou, PR China. Electronic 
      address: lsshjg@mail.sysu.edu.cn.
FAU - Xu, Xiaopeng
AU  - Xu X
AD  - MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, 
      School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute 
      of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic 
      Economic Animals, Sun Yat-sen University, Guangzhou, PR China. Electronic 
      address: xuxpeng@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180729
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Arthropod Proteins)
SB  - IM
MH  - Animals
MH  - *Arthropod Proteins/genetics/immunology
MH  - Gills/microbiology
MH  - Muscles/microbiology
MH  - *Penaeidae/genetics/immunology/microbiology
MH  - RNA Interference
MH  - Vibrio parahaemolyticus
MH  - White spot syndrome virus 1
MH  - *Zinc Fingers/genetics/immunology
OTO - NOTNLM
OT  - Immune response
OT  - Litopenaeus vannamei
OT  - Zinc finger
EDAT- 2018/08/01 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/08/01 06:00
PHST- 2018/04/10 00:00 [received]
PHST- 2018/07/18 00:00 [revised]
PHST- 2018/07/27 00:00 [accepted]
PHST- 2018/08/01 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/08/01 06:00 [entrez]
AID - S1050-4648(18)30461-3 [pii]
AID - 10.1016/j.fsi.2018.07.053 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2018 Oct;81:493-501. doi: 10.1016/j.fsi.2018.07.053. Epub 
      2018 Jul 29.