PMID- 30066700
OWN - NLM
STAT- MEDLINE
DCOM- 20181019
LR  - 20181019
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Linking)
VI  - 6
IP  - 9
DP  - 2018 Aug 21
TI  - Enhanced healing activity of burn wound infection by a dextran-HA hydrogel 
      enriched with sanguinarine.
PG  - 2472-2486
LID - 10.1039/c8bm00478a [doi]
AB  - Burn wounds are associated with a series of risks, such as infection and 
      pathologic scar tissue formation, which significantly delay wound healing and 
      lead to complications. In this study, we successfully fabricated a 
      dextran-hyaluronic acid (Dex-HA) hydrogel enriched with sanguinarine (SA) 
      incorporated into gelatin microspheres (GMs), which had high porosity, good 
      swelling ratio, enhanced NIH-3T3 fibroblast cell proliferation, and sustained SA 
      release profile. The in vitro degradation results indicate that the SA/GMs/Dex-HA 
      hydrogel can be degraded. The in vitro antibacterial tests showed that the 
      SA/GMs/Dex-HA hydrogel can inhibit methicillin-resistant Staphylococcus aureus 
      (MRSA) and Escherichia coli (E. coli). We evaluated the wound-healing effects and 
      antibacterial properties of SA/GMs/Dex-HA hydrogels in a rat full-thickness burn 
      infection model. The hematoxylin-eosin (H&E) and Masson's trichrome staining 
      results of the SA/GMs/Dex-HA hydrogel showed that it improved 
      re-epithelialization and enhanced extracellular matrix remodeling, and 
      immunohistochemistry results showed that the expression of TGF-beta1 and TNF-alpha was 
      decreased, while the TGF-beta3 expression was increased. Our findings suggest that 
      the SA/GMs/Dex-HA hydrogel provides a potential way for infected burn treatment 
      with high-quality and efficient scar inhibition.
FAU - Zhu, Qiyu
AU  - Zhu Q
AD  - Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, 
      Department of Biomedical Engineering, Jinan University, Guangzhou 510632, China. 
      guorui@jnu.edu.cn.
FAU - Jiang, Ming
AU  - Jiang M
FAU - Liu, Qiang
AU  - Liu Q
FAU - Yan, Shina
AU  - Yan S
FAU - Feng, Longbao
AU  - Feng L
FAU - Lan, Yong
AU  - Lan Y
FAU - Shan, Guiqiu
AU  - Shan G
FAU - Xue, Wei
AU  - Xue W
FAU - Guo, Rui
AU  - Guo R
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Benzophenanthridines)
RN  - 0 (Dextrans)
RN  - 0 (Hydrogels)
RN  - 0 (Isoquinolines)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9000-70-8 (Gelatin)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - AV9VK043SS (sanguinarine)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*administration & dosage/chemistry
MH  - Benzophenanthridines/*administration & dosage/chemistry
MH  - Burns/drug therapy
MH  - Cell Survival/drug effects
MH  - Dextrans/*administration & dosage/chemistry
MH  - Escherichia coli/drug effects/growth & development
MH  - Gelatin/administration & dosage/chemistry
MH  - Hyaluronic Acid/*administration & dosage/chemistry
MH  - Hydrogels/*administration & dosage/chemistry
MH  - Isoquinolines/*administration & dosage/chemistry
MH  - Male
MH  - Methicillin-Resistant Staphylococcus aureus/drug effects/growth & development
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Rats, Sprague-Dawley
MH  - Skin/drug effects/immunology/pathology
MH  - Staphylococcal Infections/*drug therapy/immunology/pathology
MH  - Transforming Growth Factor beta/immunology
MH  - Tumor Necrosis Factor-alpha/immunology
MH  - Wound Healing/drug effects
MH  - Wound Infection/*drug therapy/immunology/pathology
EDAT- 2018/08/02 06:00
MHDA- 2018/10/20 06:00
CRDT- 2018/08/02 06:00
PHST- 2018/08/02 06:00 [pubmed]
PHST- 2018/10/20 06:00 [medline]
PHST- 2018/08/02 06:00 [entrez]
AID - 10.1039/c8bm00478a [doi]
PST - ppublish
SO  - Biomater Sci. 2018 Aug 21;6(9):2472-2486. doi: 10.1039/c8bm00478a.