PMID- 30066819
OWN - NLM
STAT- MEDLINE
DCOM- 20190516
LR  - 20220408
IS  - 1984-0462 (Electronic)
IS  - 0103-0582 (Print)
IS  - 0103-0582 (Linking)
VI  - 36
IP  - 3
DP  - 2018 Jul-Sep
TI  - VITAMIN D RECEPTOR GENE MUTATIONS AND VITAMIN D SERUM LEVELS IN ASTHMATIC 
      CHILDREN.
PG  - 269-274
LID - 10.1590/1984-0462/;2018;36;3;00016 [doi]
AB  - OBJECTIVE: To verify the relationship between polymorphisms of the vitamin D 
      receptor gene (VDR), clinical findings, and serum vitamin D (VD) levels in 
      asthmatics. METHODS: A cross sectional study of 77 children aged 7 to 14 years 
      old, who were attended at a specialized clinic. The children were divided into 3 
      groups: asthmatics who had been using inhaled corticosteroids (ICS) for more than 
      one year; asthmatics who had not been using ICS; non-asthmatics, and children 
      without allergies (according to the International Study of Asthma and Allergies 
      in Childhood -- ISAAC). Spirometry, skin prick tests, the presence of a VDR 
      promoter CDX2 polymorphism from an allele-specific polimerase chain reaction 
      (PCR), exons 2 and 3 polymorphisms genotyping by PCR-SSCA (single-strand 
      conformational analysis), total immunoglobulin E (IgE) and specific IgE to mites 
      and grass were evaluated in these three groups. Levels of 25-hydroxyvitamin D 
      were determined in asthmatics only. RESULTS: The mean age of the children was 
      10.8+/-2.0 years old, 57% were male, 38 were asthmatic and using ICS, 22 were 
      asthmatic and not using ICS, and 17 were non-asthmatic. Allergic rhinitis was 
      present in 90% of asthmatics. Homozygous CDX2 was detected in 23% of the patients 
      and absent in the control group (p=0.03). Lower forced expiratory volume in 1 
      second (FEV1%) values were observed in CDX2 homozygous asthmatics (p=0.001). 
      Variations in the exon 2 and 3 sequences were not related to asthma or the other 
      tests. VD deficiency or insufficiency was detected in 98% of asthmatics. There 
      was no association between VD levels and genetic polymorphisms from exons 2 and 
      3. CONCLUSIONS: There was a positive association between homozygous CDX2 
      polymorphism, asthma and lower FEV1% values. CDX2 is capable of modifying cell 
      interaction between VDR and VD, and it could be associated with the prevalence of 
      asthma, and the difficulty in controlling the disease.
FAU - Santos, Hevertton Luiz Bozzo Silva
AU  - Santos HLBS
AD  - Universidade Federal do Parana, Curitiba, PR, Brasil.
FAU - Silva, Silvia de Souza E
AU  - Silva SSE
AD  - Faculdades Integradas do Brasil, Curitiba, PR, Brasil.
FAU - Paula, Estela de
AU  - Paula E
AD  - Faculdades Integradas do Brasil, Curitiba, PR, Brasil.
FAU - Pereira-Ferrari, Lilian
AU  - Pereira-Ferrari L
AD  - Centro Universitario Autonomo do Brasil, Curitiba, PR, Brasil.
FAU - Mikami, Liya
AU  - Mikami L
AD  - Centro Universitario Autonomo do Brasil, Curitiba, PR, Brasil.
FAU - Riedi, Carlos Antonio
AU  - Riedi CA
AD  - Universidade Federal do Parana, Curitiba, PR, Brasil.
FAU - Chong-Neto, Herberto Jose
AU  - Chong-Neto HJ
AD  - Universidade Federal do Parana, Curitiba, PR, Brasil.
FAU - Rosario, Nelson Augusto
AU  - Rosario NA
AD  - Universidade Federal do Parana, Curitiba, PR, Brasil.
LA  - por
LA  - eng
PT  - Journal Article
PT  - Observational Study
TT  - MUTACOES DO GENE DO RECEPTOR DE VITAMINA D E NIVEIS SERICOS DE VITAMINA D EM 
      CRIANCAS COM ASMA.
DEP - 20180726
PL  - Brazil
TA  - Rev Paul Pediatr
JT  - Revista paulista de pediatria : orgao oficial da Sociedade de Pediatria de Sao 
      Paulo
JID - 9109353
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (VDR protein, human)
RN  - 1406-16-2 (Vitamin D)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adolescent
MH  - Adrenal Cortex Hormones/therapeutic use
MH  - Asthma/*blood/drug therapy
MH  - Calcium/blood
MH  - Child
MH  - Cross-Sectional Studies
MH  - Female
MH  - Humans
MH  - Male
MH  - Mutation
MH  - Polymorphism, Genetic
MH  - Receptors, Calcitriol/*genetics
MH  - Vitamin D/*blood
PMC - PMC6202901
COIS- Conflict of interests: The authors declare no conflict of interests.
EDAT- 2018/08/02 06:00
MHDA- 2019/05/17 06:00
PMCR- 2018/07/01
CRDT- 2018/08/02 06:00
PHST- 2017/04/12 00:00 [received]
PHST- 2017/09/22 00:00 [accepted]
PHST- 2018/08/02 06:00 [pubmed]
PHST- 2019/05/17 06:00 [medline]
PHST- 2018/08/02 06:00 [entrez]
PHST- 2018/07/01 00:00 [pmc-release]
AID - S0103-05822018005008102 [pii]
AID - 10.1590/1984-0462/;2018;36;3;00016 [doi]
PST - ppublish
SO  - Rev Paul Pediatr. 2018 Jul-Sep;36(3):269-274. doi: 
      10.1590/1984-0462/;2018;36;3;00016. Epub 2018 Jul 26.