PMID- 30066875
OWN - NLM
STAT- MEDLINE
DCOM- 20181108
LR  - 20210916
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 18
IP  - 3
DP  - 2018 Sep
TI  - Murine and Chinese cobra venom‑derived nerve growth factor stimulate chondrogenic 
      differentiation of BMSCs in vitro: A comparative study.
PG  - 3341-3349
LID - 10.3892/mmr.2018.9307 [doi]
AB  - Mesenchymal stem cell (MSC)‑based therapy has been commonly used in cartilage 
      reconstruction, due to its self‑renewing ability and multi‑differentiation 
      potential. Nerve growth factor (NGF) from cobra venom has been reported to 
      regulate chondrogenesis of bone‑derived MSCs (BMSCs) and chondrocyte metabolism. 
      Therefore, the present study aimed to determine whether other sources of NGF 
      behave in the same manner as NGF from natural venom. The present study compared 
      the effects of NGF from two sources, the commercially purchased recombinant 
      murine beta‑NGF (mNGF) and cobra venom‑derived NGF (cvNGF), on chondrogenesis of 
      BMSCs by performing hematoxylin and eosin and fluorescein diacetate/propidium 
      iodide staining, DNA and glycosaminoglycan quantization and reverse 
      transcription‑quantitative polymerase chain reaction to investigate cell 
      morphology, viability, proliferation, glycosaminoglycan synthesis and 
      cartilage‑specific gene expression. The results demonstrated that cvNGF 
      significantly accelerated cell proliferation and upregulated the expression of 
      cartilage‑specific genes, including aggrecan, SRY‑box 9 and collagen type II alpha1 
      chain. Conversely, cvNGF reduced the expression levels of collagen type I alpha1 
      chain (a fibrocartilage marker), runt‑related transcription factor 2 and enolase 
      2 compared with in the mNGF and control groups. In addition, Chinese cobra venom, 
      which is the main resource of cvNGF, is abundant and inexpensive, thus greatly 
      decreasing the cost. In conclusion, the present study demonstrated that cvNGF may 
      be considered a potential growth factor for inducing chondrogenic differentiation 
      of BMSCs.
FAU - Miao, Zhikang
AU  - Miao Z
AD  - Guangxi Engineering Center in Biomedical Material for Tissue and Organ 
      Regeneration, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
FAU - Lu, Zhenhui
AU  - Lu Z
AD  - Guangxi Engineering Center in Biomedical Material for Tissue and Organ 
      Regeneration, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
FAU - Luo, Shixing
AU  - Luo S
AD  - Department of Orthopedics, Ninth Affiliated Hospital of Guangxi Medical 
      University, Beihai 536000, P.R. China.
FAU - Lei, Danqing
AU  - Lei D
AD  - Life Sciences Institute, Guangxi Medical University, Nanning, Guangxi 530021, 
      P.R. China.
FAU - He, Yi
AU  - He Y
AD  - Guangxi Engineering Center in Biomedical Material for Tissue and Organ 
      Regeneration, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
FAU - Wu, Huayu
AU  - Wu H
AD  - Department of Cell Biology and Genetics, School of Premedical Sciences, Guangxi 
      Medical University, Nanning, Guangxi 530021, P.R. China.
FAU - Zhao, Jinmin
AU  - Zhao J
AD  - Guangxi Engineering Center in Biomedical Material for Tissue and Organ 
      Regeneration, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
FAU - Zheng, Li
AU  - Zheng L
AD  - Guangxi Engineering Center in Biomedical Material for Tissue and Organ 
      Regeneration, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180723
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Biomarkers)
RN  - 0 (Elapid Venoms)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Recombinant Proteins)
RN  - 9007-34-5 (Collagen)
SB  - IM
EIN - Mol Med Rep. 2021 Nov;24(5):. PMID: 34528695
MH  - Animals
MH  - Biomarkers
MH  - Cell Differentiation/*drug effects/genetics
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Chondrogenesis/*drug effects/genetics
MH  - Collagen/biosynthesis
MH  - Elapid Venoms/chemistry/*pharmacology
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental
MH  - Immunohistochemistry
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/*drug effects/metabolism
MH  - Mice
MH  - Nerve Growth Factors/chemistry/*pharmacology
MH  - Rats
MH  - Recombinant Proteins
PMC - PMC6102669
EDAT- 2018/08/02 06:00
MHDA- 2018/11/09 06:00
PMCR- 2018/07/23
CRDT- 2018/08/02 06:00
PHST- 2017/12/23 00:00 [received]
PHST- 2018/06/22 00:00 [accepted]
PHST- 2018/08/02 06:00 [pubmed]
PHST- 2018/11/09 06:00 [medline]
PHST- 2018/08/02 06:00 [entrez]
PHST- 2018/07/23 00:00 [pmc-release]
AID - mmr-18-03-3341 [pii]
AID - 10.3892/mmr.2018.9307 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Sep;18(3):3341-3349. doi: 10.3892/mmr.2018.9307. Epub 2018 Jul 
      23.