PMID- 30066931
OWN - NLM
STAT- MEDLINE
DCOM- 20181108
LR  - 20190816
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 18
IP  - 3
DP  - 2018 Sep
TI  - NSD2 is downregulated in T2DM and promotes beta cell proliferation and insulin 
      secretion through the transcriptionally regulation of PDX1.
PG  - 3513-3520
LID - 10.3892/mmr.2018.9338 [doi]
AB  - Diabetes has become a major public health issue in the world. Type 2 diabetes 
      mellitus (T2DM), also known as non‑insulin‑dependent diabetes mellitus, has been 
      identified to result in an inability to compensate for insulin resistance. A 
      previous study has shown that NSD2 regulates glucose metabolism; however, whether 
      NSD2 serves roles in diabetes has not been thoroughly elucidated to date. In 
      present study, the expression of NSD2 in blood samples from patients with T2DM 
      was compared with that in healthy volunteers. Notably, the expression of NSD2 was 
      negatively correlated with glucose concentration but positively associated with 
      PDX1 expression. Several functional experiments, including CCK‑8 assay and colony 
      formation assay, revealed that NSD2 promoted the proliferation of pancreatic beta 
      cell lines. Moreover, ectopic expression of NSD2 significantly promoted insulin 
      secretion. In addition, NSD2 served as a transfection factor and it was 
      identified that NSD2 transcriptionally regulated PDX1 expression through its 
      H3K36me2 methyltransferase activity. The present study indicated that NSD2 may be 
      a novel molecular therapy target of T2DM.
FAU - Shi, Suqin
AU  - Shi S
AD  - Department of Endocrinology, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, Henan 450052, P.R. China.
FAU - Zhao, Lu
AU  - Zhao L
AD  - Department of Endocrinology, The Third Affiliated Hospital of Henan University of 
      TCM, Zhengzhou, Henan 450004, P.R. China.
FAU - Zheng, Lili
AU  - Zheng L
AD  - Department of Endocrinology, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, Henan 450052, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180731
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Histones)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Insulin)
RN  - 0 (Repressor Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (pancreatic and duodenal homeobox 1 protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
RN  - EC 2.1.1.43 (NSD2 protein, human)
SB  - IM
MH  - Adult
MH  - Cell Line
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Diabetes Mellitus, Type 2/*genetics/*metabolism
MH  - Female
MH  - *Gene Expression Regulation
MH  - Histone-Lysine N-Methyltransferase/*genetics/metabolism
MH  - Histones/metabolism
MH  - Homeodomain Proteins/*genetics/metabolism
MH  - Humans
MH  - Insulin/*metabolism
MH  - Insulin Secretion
MH  - Insulin-Secreting Cells/*metabolism
MH  - Male
MH  - Methylation
MH  - Middle Aged
MH  - Repressor Proteins/*genetics/metabolism
MH  - Trans-Activators/*genetics/metabolism
MH  - Transcription, Genetic
MH  - Young Adult
EDAT- 2018/08/02 06:00
MHDA- 2018/11/09 06:00
CRDT- 2018/08/02 06:00
PHST- 2018/02/10 00:00 [received]
PHST- 2018/06/27 00:00 [accepted]
PHST- 2018/08/02 06:00 [pubmed]
PHST- 2018/11/09 06:00 [medline]
PHST- 2018/08/02 06:00 [entrez]
AID - 10.3892/mmr.2018.9338 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Sep;18(3):3513-3520. doi: 10.3892/mmr.2018.9338. Epub 2018 Jul 
      31.