PMID- 30067086 OWN - NLM STAT- MEDLINE DCOM- 20181218 LR - 20181218 IS - 1480-3321 (Electronic) IS - 0831-2796 (Linking) VI - 61 IP - 9 DP - 2018 Sep TI - FISH analysis of Zanthoxylum armatum based on oligonucleotides for 5S rDNA and (GAA)(6). PG - 699-702 LID - 10.1139/gen-2018-0009 [doi] AB - Fluorescence in situ hybridization (FISH) using oligonucleotide probes for (GAA)(6) (18 bp) and ribosomal DNA (rDNA) (5S rDNA, 41 bp) was applied to analyse Zanthoxylum armatum. (GAA)(6) loci were detected on the pericentromeric regions of five chromosome pairs, and 5S rDNA loci were also detected on the pericentromeric regions of another two chromosome pairs. The densities and locations of (GAA)(6) and 5S rDNA signals varied between individual chromosomes. High-intensity (GAA)(6) signals were detected at the centromeres of two large and two smaller metacentric chromosomes. Relatively strong (GAA)(6) signals were detected at the centromeres of two relatively small metacentric chromosomes, although strong 5S rDNA signals were detected at the centromeres of two additional smaller metacentric chromosomes. Weak (GAA)(6) signals were detected at the centromeres of four large metacentric chromosomes, whereas weak 5S rDNA signals were detected at the centromeres of two smaller metacentric chromosomes. The remaining chromosomes exhibited no signals. Zanthoxylum armatum had 2n = approximately 128. The lengths of the mitotic metaphase chromosomes ranged from 1.22 to 2.34 mum. Our results provide information that may be beneficial for future cytogenetic studies and could contribute to the physical assembly of the Zanthoxylum genome. FAU - Luo, Xiaomei AU - Luo X AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. FAU - Liu, Juncheng AU - Liu J AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. FAU - Wang, Jingyan AU - Wang J AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. FAU - Gong, Wei AU - Gong W AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. FAU - Chen, Liang AU - Chen L AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. FAU - Wan, Wenlin AU - Wan W AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. AD - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China. LA - eng PT - Journal Article DEP - 20180801 PL - Canada TA - Genome JT - Genome JID - 8704544 RN - 0 (RNA, Ribosomal, 5S) SB - IM MH - Centromere/genetics MH - Chromosomes, Plant/*genetics MH - In Situ Hybridization, Fluorescence MH - RNA, Ribosomal, 5S/*genetics MH - *Trinucleotide Repeats MH - Zanthoxylum/*genetics OTO - NOTNLM OT - ADNr OT - GAA OT - Zanthoxylum species OT - especes du genre Zanthoxylum OT - rDNA OT - trinucleotide OT - trinucleotide EDAT- 2018/08/02 06:00 MHDA- 2018/12/19 06:00 CRDT- 2018/08/02 06:00 PHST- 2018/08/02 06:00 [pubmed] PHST- 2018/12/19 06:00 [medline] PHST- 2018/08/02 06:00 [entrez] AID - 10.1139/gen-2018-0009 [doi] PST - ppublish SO - Genome. 2018 Sep;61(9):699-702. doi: 10.1139/gen-2018-0009. Epub 2018 Aug 1.