PMID- 30068872
OWN - NLM
STAT- MEDLINE
DCOM- 20181016
LR  - 20181016
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 41
IP  - 8
DP  - 2018
TI  - Dibenzoylmethane, a Component of Licorice, Suppresses Monocyte-to-Macrophage 
      Differentiation and Inflammatory Responses in Human Monocytes and Mouse 
      Macrophages.
PG  - 1228-1236
LID - 10.1248/bpb.b18-00064 [doi]
AB  - The objective of this study was to investigate the effect of dibenzoylmethane 
      (DBM) on monocyte-to-macrophage differentiation, the inflammatory response, and 
      the resulting signaling in human monocytes and murine macrophage. DBM effectively 
      inhibited the monocyte-to-macrophage differentiation induced by phorbol 
      12-myristate 13-acetate (PMA) through a reduction in adhesion of THP-1 cells. 
      Cluster of differentiation molecule beta (CD11beta) and CD36, which are surface markers 
      of macrophage differentiation, were downregulated by 80 and 74%, respectively. 
      DBM also significantly inhibited lipopolysaccharide (LPS)-induced nitrite (NO) 
      production through the downregulation of inducible oxide synthase (iNOS) in 
      RAW264.7 cells. The abundance of cyclooxygenase-2 (COX-2), a pro-inflammatory 
      protein, was also effectively decreased by DBM in a dose-dependent manner. DBM 
      (50 microM) reduced the levels of COX-2 and iNOS by 81 and 78%, respectively. DBM 
      significantly inhibited the translocation of nuclear factor 
      kappa-light-chain-enhancer of activated B cells (NF-kappaB), an inflammatory 
      transcription factor, into the nucleus. DBM-mediated increase of NF-kappaB 
      translocation resulted from the DBM-induced suppression of the phosphorylation of 
      nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, 
      alpha (IkappaBalpha). In contrast, DBM effectively increased the expression of nuclear 
      factor E2-related factor 2 (Nrf2) and its target protein, hemeoxygenase-1 (HO-1). 
      Nrf2 translocation into the nucleus was also significantly enhanced by DBM. 
      Furthermore, DBM effectively inhibited the expression of pro-inflammatory 
      cytokines such as tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta 
      (IL-1beta), IL-6, and monocyte chemoattractant protein-1 (MCP-1). These results 
      indicated that the DBM-mediated differential regulation of NF-kappaB and Nrf2, which 
      are major transcription factors involved in inflammation, inhibited the 
      expression of inflammatory cytokines.
FAU - Kang, Bobin
AU  - Kang B
AD  - Department of Public Health Sciences, Korea University.
FAU - Kim, Joo Hyoun
AU  - Kim JH
AD  - Department of Food Science and Technology, College of Natural Science, Seoul 
      Women's University.
FAU - Kim, Chae Young
AU  - Kim CY
AD  - Department of Public Health Sciences, Korea University.
FAU - Hong, Jungil
AU  - Hong J
AD  - Department of Food Science and Technology, College of Natural Science, Seoul 
      Women's University.
FAU - Choi, Hyeon-Son
AU  - Choi HS
AD  - Department of Food Science and Technology, College of Natural Science, Seoul 
      Women's University.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Chalcones)
RN  - 0 (Cytokines)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - ANS7ME8OKC (dibenzoylmethane)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Adhesion/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Chalcones/*pharmacology
MH  - Cytokines/genetics
MH  - Glycyrrhiza
MH  - Humans
MH  - Macrophages/cytology/*drug effects/metabolism
MH  - Mice
MH  - Monocytes/cytology/*drug effects
MH  - NF-E2-Related Factor 2/metabolism
MH  - NF-kappa B/metabolism
MH  - RAW 264.7 Cells
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - RAW264.7
OT  - THP-1
OT  - cytokine
OT  - dibenzoylmethane
OT  - inflammatory response
OT  - monocyte-to-macrophage differentiation
EDAT- 2018/08/03 06:00
MHDA- 2018/10/17 06:00
CRDT- 2018/08/03 06:00
PHST- 2018/08/03 06:00 [entrez]
PHST- 2018/08/03 06:00 [pubmed]
PHST- 2018/10/17 06:00 [medline]
AID - 10.1248/bpb.b18-00064 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2018;41(8):1228-1236. doi: 10.1248/bpb.b18-00064.