PMID- 30068940
OWN - NLM
STAT- MEDLINE
DCOM- 20190228
LR  - 20230309
IS  - 1476-5594 (Electronic)
IS  - 0950-9232 (Linking)
VI  - 37
IP  - 50
DP  - 2018 Dec
TI  - PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the 
      splicing balance of NEAT1 and pre-miR-612.
PG  - 6399-6413
LID - 10.1038/s41388-018-0416-8 [doi]
AB  - Nuclear-enriched RNA-binding proteins (RBPs) are mainly involved in 
      transcriptional regulation, which is a critical checkpoint to tune gene diversity 
      and expression levels. We analyzed nuclear RBPs in human HCC tissues and matched 
      normal control tissues. Based on the gene expression levels, PTBP3 was identified 
      as top-ranked in the nuclei of HCC cells. HCC cell lines then were transfected 
      with siRNAs or lentiviral vectors. PTBP3 promoted HCC cell proliferation and 
      metastasis both in vitro and in vivo. RNA immunoprecipitation (RIP), fluorescence 
      in situ hybridization (FISH) and qRT-PCR assays verified that PTBP3 protein 
      recruited abundant lnc-NEAT1 splicing variants (NEAT1_1 and NEAT1_2) and 
      pre-miR-612 (precursor of miR-612) in the nucleus. NEAT1_1, NEAT1_2 and miR-612 
      expression levels were determined by PTBP3. Correlational analyses revealed that 
      PTBP3 was positively correlated with NEAT1, but it was inversely correlated with 
      miR-612 in HCC. The P53/CCND1 and AKT2/EMT pathways were determined by NEAT1 and 
      miR-612 respectively in HCC. The PTBP3(high) and NEAT1(high)/miR-612(low) 
      patients had a shorter overall survival. Therefore, nuclear-enriched RBP, PTBP3, 
      promotes HCC cell malignant growth and metastasis by regulating the balance of 
      splicing variants (NEAT1_1, NEAT1_2 and miR-612) in HCC.
FAU - Yang, Xisheng
AU  - Yang X
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Qu, Shibin
AU  - Qu S
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
AD  - Section of General Surgery, Department of Surgery, University of Michigan, Ann 
      Arbor, Michigan, USA.
FAU - Wang, Lin
AU  - Wang L
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Zhang, Hongtao
AU  - Zhang H
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Yang, Zhaoxu
AU  - Yang Z
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Wang, Jianlin
AU  - Wang J
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Dai, Bin
AU  - Dai B
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Tao, Kaishan
AU  - Tao K
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Shang, Runze
AU  - Shang R
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Liu, Zhengcai
AU  - Liu Z
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Li, Xiao
AU  - Li X
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Zhang, Zhuochao
AU  - Zhang Z
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Xia, Congcong
AU  - Xia C
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Ma, Ben
AU  - Ma B
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Liu, Wei
AU  - Liu W
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China.
FAU - Li, Haimin
AU  - Li H
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China. lihaim@fmmu.edu.cn.
FAU - Dou, Kefeng
AU  - Dou K
AD  - Department of Hepatobiliary Surgery, The Xijing Hospital of The Fourth Military 
      Medical University, Xi'an, China. doukef@fmmu.edu.cn.
LA  - eng
GR  - 81672339/National Natural Science Foundation of China (National Science 
      Foundation of China)/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20180801
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (MALAT1 long non-coding RNA, human)
RN  - 0 (MIRN612 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NEAT1 long non-coding RNA, human)
RN  - 0 (PTBP3 protein, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 139076-35-0 (Polypyrimidine Tract-Binding Protein)
SB  - IM
RIN - Oncogene. 2023 Apr;42(15):1263. PMID: 36890279
MH  - Animals
MH  - Carcinoma, Hepatocellular/metabolism/*pathology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Humans
MH  - Liver Neoplasms/metabolism/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*genetics/metabolism
MH  - Polypyrimidine Tract-Binding Protein/*metabolism
MH  - RNA, Long Noncoding/*genetics/metabolism
EDAT- 2018/08/03 06:00
MHDA- 2019/03/01 06:00
CRDT- 2018/08/03 06:00
PHST- 2017/12/31 00:00 [received]
PHST- 2018/06/29 00:00 [accepted]
PHST- 2018/05/31 00:00 [revised]
PHST- 2018/08/03 06:00 [pubmed]
PHST- 2019/03/01 06:00 [medline]
PHST- 2018/08/03 06:00 [entrez]
AID - 10.1038/s41388-018-0416-8 [pii]
AID - 10.1038/s41388-018-0416-8 [doi]
PST - ppublish
SO  - Oncogene. 2018 Dec;37(50):6399-6413. doi: 10.1038/s41388-018-0416-8. Epub 2018 
      Aug 1.