PMID- 30068989
OWN - NLM
STAT- MEDLINE
DCOM- 20191203
LR  - 20191203
IS  - 2041-4889 (Electronic)
VI  - 9
IP  - 8
DP  - 2018 Aug 1
TI  - Rsad2 is necessary for mouse dendritic cell maturation via the IRF7-mediated 
      signaling pathway.
PG  - 823
LID - 10.1038/s41419-018-0889-y [doi]
LID - 823
AB  - Dendritic cells (DCs) are the most potent professional antigen presenting cells 
      and inducers of T cell-mediated immunity. However, few specific markers of mature 
      DCs (mDC) have been reported. A previous microarray analysis revealed expression 
      of mDC-specific genes and identified Rsad2 (radical S-adenosyl methionine domain 
      containing 2) as a candidate specific marker for DC maturation. Mouse bone 
      marrow-derived DCs were transfected with Rsad2 siRNA and examined by flow 
      cytometry, ELISA, western, and confocal microscopy. C57BL/6 mice received 
      intravenously B16F10 cells to establish a pulmonary metastasis model. 
      Tumor-bearing mice then received subcutaneously two injections of mDCs or Rsad2 
      knockdown DCs. The cytotoxic T lymphocyte (CTL) population was examined from 
      splenocytes of DC-vaccinated mice by flow cytometry. Rsad2 was induced at high 
      levels in LPS-stimulated mDCs and mDC function was markedly attenuated under 
      conditions of Rsad2 knockdown. Moreover, Rsad2 was necessary for mDC maturation 
      via the IRF7-mediated signaling pathway. The importance of Rsad2 was confirmed in 
      an Rsad2 knockdown lung metastasis mouse model in which mDCs lost their antitumor 
      efficacy. Data on the CTL population further supported the results as above. 
      Taken together, Rsad2 was an obvious and specific marker necessary for DC 
      maturation and these findings will be clearly helpful for further understanding 
      of DC biology.
FAU - Jang, Ji-Su
AU  - Jang JS
AD  - Department of Biotechnology, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam, 
      Gyeonggi-do, 13488, Republic of Korea.
FAU - Lee, Jun-Ho
AU  - Lee JH
AD  - Department of Biotechnology, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam, 
      Gyeonggi-do, 13488, Republic of Korea.
AD  - Pharos Vaccine Inc., 545 Dunchon-daero, Jungwon-gu, Seongnam, Gyeonggi-do, 13215, 
      Republic of Korea.
FAU - Jung, Nam-Chul
AU  - Jung NC
AD  - Pharos Vaccine Inc., 545 Dunchon-daero, Jungwon-gu, Seongnam, Gyeonggi-do, 13215, 
      Republic of Korea.
FAU - Choi, So-Yeon
AU  - Choi SY
AD  - Department of Biotechnology, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam, 
      Gyeonggi-do, 13488, Republic of Korea.
FAU - Park, Soo-Yeoun
AU  - Park SY
AD  - Department of Biotechnology, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam, 
      Gyeonggi-do, 13488, Republic of Korea.
FAU - Yoo, Ji-Young
AU  - Yoo JY
AD  - Department of Biotechnology, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam, 
      Gyeonggi-do, 13488, Republic of Korea.
FAU - Song, Jie-Young
AU  - Song JY
AUID- ORCID: 0000-0003-2434-7511
AD  - Department of Radiation Cancer Sciences, Korea Institute of Radiological and 
      Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul, 01812, Republic of Korea.
FAU - Seo, Han Geuk
AU  - Seo HG
AD  - Department of Food Science and Biotechnology of Animal Products, Sanghuh College 
      of Life Sciences, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, 
      Republic of Korea.
FAU - Lee, Hyun Soo
AU  - Lee HS
AD  - Pharos Vaccine Inc., 545 Dunchon-daero, Jungwon-gu, Seongnam, Gyeonggi-do, 13215, 
      Republic of Korea.
FAU - Lim, Dae-Seog
AU  - Lim DS
AD  - Department of Biotechnology, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam, 
      Gyeonggi-do, 13488, Republic of Korea. dslim@cha.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180801
PL  - England
TA  - Cell Death Dis
JT  - Cell death & disease
JID - 101524092
RN  - 0 (Interferon Regulatory Factor-7)
RN  - 0 (Interferon Type I)
RN  - 0 (Irf7 protein, mouse)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Rsad2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Cell Line, Tumor
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Interferon Regulatory Factor-7/*metabolism
MH  - Interferon Type I/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lung Neoplasms/immunology/secondary/therapy
MH  - Male
MH  - Melanoma, Experimental/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Proteins/antagonists & inhibitors/genetics/*metabolism
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - Signal Transduction
MH  - T-Lymphocytes, Cytotoxic/cytology/immunology
PMC - PMC6070531
COIS- The authors declare that they have no conflict of interest.
EDAT- 2018/08/03 06:00
MHDA- 2019/12/04 06:00
PMCR- 2018/08/01
CRDT- 2018/08/03 06:00
PHST- 2018/06/23 00:00 [received]
PHST- 2018/07/17 00:00 [accepted]
PHST- 2018/07/17 00:00 [revised]
PHST- 2018/08/03 06:00 [entrez]
PHST- 2018/08/03 06:00 [pubmed]
PHST- 2019/12/04 06:00 [medline]
PHST- 2018/08/01 00:00 [pmc-release]
AID - 10.1038/s41419-018-0889-y [pii]
AID - 889 [pii]
AID - 10.1038/s41419-018-0889-y [doi]
PST - epublish
SO  - Cell Death Dis. 2018 Aug 1;9(8):823. doi: 10.1038/s41419-018-0889-y.