PMID- 30069008
OWN - NLM
STAT- MEDLINE
DCOM- 20191203
LR  - 20220409
IS  - 2041-4889 (Electronic)
VI  - 9
IP  - 8
DP  - 2018 Aug 1
TI  - Screening for long noncoding RNAs associated with oral squamous cell carcinoma 
      reveals the potentially oncogenic actions of DLEU1.
PG  - 826
LID - 10.1038/s41419-018-0893-2 [doi]
LID - 826
AB  - Recent studies have shown that long noncoding RNAs (lncRNAs) have pivotal roles 
      in human malignancies, although their significance in oral squamous cell 
      carcinoma (OSCC) is not fully understood. In the present study, we identified 
      lncRNAs functionally associated with OSCC. By analyzing RNA-seq datasets obtained 
      from primary head and neck squamous cell carcinoma (HNSCC), we identified 15 
      lncRNAs aberrantly expressed in cancer tissues. We then validated their 
      expression in 18 OSCC cell lines using qRT-PCR and identified 6 lncRNAs 
      frequently overexpressed in OSCC. Among those, we found that knocking down DLEU1 
      (deleted in lymphocytic leukemia 1) strongly suppressed OSCC cell proliferation. 
      DLEU1 knockdown also suppressed migration, invasion, and xenograft formation by 
      OSCC cells, which is suggestive of its oncogenic functionality. Microarray 
      analysis revealed that DLEU1 knockdown significantly affects expression of a 
      number of cancer-related genes in OSCC cells, including HAS3, CD44, and TP63, 
      suggesting that DLEU1 regulates HA-CD44 signaling. Expression of DLEU1 was 
      elevated in 71% of primary OSCC tissues, and high DLEU1 expression was associated 
      with shorter overall survival of HNSCC patients. These data suggest that elevated 
      DLEU1 expression contributes to OSCC development, and that DLEU1 may be a useful 
      therapeutic target in OSCC.
FAU - Nishiyama, Koyo
AU  - Nishiyama K
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, S1, 
      W16, Chuo-ku, Sapporo, 060-8543, Japan.
AD  - Department of Molecular Biology, Sapporo Medical University School of Medicine, 
      S1, W17, Chuo-ku, Sapporo, 060-8556, Japan.
FAU - Maruyama, Reo
AU  - Maruyama R
AD  - Project for Cancer Epigenomics, Cancer Institute, Japanese Foundation for Cancer 
      Research, 3-8-31, Ariake, Koto-ku, Tokyo, 135-8550, Japan.
FAU - Niinuma, Takeshi
AU  - Niinuma T
AD  - Department of Molecular Biology, Sapporo Medical University School of Medicine, 
      S1, W17, Chuo-ku, Sapporo, 060-8556, Japan.
FAU - Kai, Masahiro
AU  - Kai M
AD  - Department of Molecular Biology, Sapporo Medical University School of Medicine, 
      S1, W17, Chuo-ku, Sapporo, 060-8556, Japan.
FAU - Kitajima, Hiroshi
AU  - Kitajima H
AD  - Department of Molecular Biology, Sapporo Medical University School of Medicine, 
      S1, W17, Chuo-ku, Sapporo, 060-8556, Japan.
FAU - Toyota, Mutsumi
AU  - Toyota M
AD  - Department of Molecular Biology, Sapporo Medical University School of Medicine, 
      S1, W17, Chuo-ku, Sapporo, 060-8556, Japan.
FAU - Hatanaka, Yui
AU  - Hatanaka Y
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, S1, 
      W16, Chuo-ku, Sapporo, 060-8543, Japan.
AD  - Department of Molecular Biology, Sapporo Medical University School of Medicine, 
      S1, W17, Chuo-ku, Sapporo, 060-8556, Japan.
FAU - Igarashi, Tomohiro
AU  - Igarashi T
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, S1, 
      W16, Chuo-ku, Sapporo, 060-8543, Japan.
FAU - Kobayashi, Jun-Ichi
AU  - Kobayashi JI
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, S1, 
      W16, Chuo-ku, Sapporo, 060-8543, Japan.
FAU - Ogi, Kazuhiro
AU  - Ogi K
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, S1, 
      W16, Chuo-ku, Sapporo, 060-8543, Japan.
FAU - Dehari, Hironari
AU  - Dehari H
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, S1, 
      W16, Chuo-ku, Sapporo, 060-8543, Japan.
FAU - Miyazaki, Akihiro
AU  - Miyazaki A
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, S1, 
      W16, Chuo-ku, Sapporo, 060-8543, Japan.
FAU - Yorozu, Akira
AU  - Yorozu A
AD  - Department of Molecular Biology, Sapporo Medical University School of Medicine, 
      S1, W17, Chuo-ku, Sapporo, 060-8556, Japan.
FAU - Yamamoto, Eiichiro
AU  - Yamamoto E
AD  - Department of Molecular Biology, Sapporo Medical University School of Medicine, 
      S1, W17, Chuo-ku, Sapporo, 060-8556, Japan.
FAU - Idogawa, Masashi
AU  - Idogawa M
AUID- ORCID: 0000-0002-8507-1726
AD  - Department of Medical Genome Science, Research Institute for Frontier Medicine, 
      Sapporo Medical University School of Medicine, S1, W17, Chuo-ku, Sapporo, 
      060-8556, Japan.
FAU - Sasaki, Yasushi
AU  - Sasaki Y
AD  - Department of Medical Genome Science, Research Institute for Frontier Medicine, 
      Sapporo Medical University School of Medicine, S1, W17, Chuo-ku, Sapporo, 
      060-8556, Japan.
FAU - Sugai, Tamotsu
AU  - Sugai T
AD  - Department of Molecular Diagnostic Pathology, School of Medicine, Iwate Medical 
      University, Morioka, Japan.
FAU - Tokino, Takashi
AU  - Tokino T
AD  - Department of Medical Genome Science, Research Institute for Frontier Medicine, 
      Sapporo Medical University School of Medicine, S1, W17, Chuo-ku, Sapporo, 
      060-8556, Japan.
FAU - Hiratsuka, Hiroyoshi
AU  - Hiratsuka H
AD  - Department of Oral Surgery, Sapporo Medical University School of Medicine, S1, 
      W16, Chuo-ku, Sapporo, 060-8543, Japan.
FAU - Suzuki, Hiromu
AU  - Suzuki H
AUID- ORCID: 0000-0001-9635-3238
AD  - Department of Molecular Biology, Sapporo Medical University School of Medicine, 
      S1, W17, Chuo-ku, Sapporo, 060-8556, Japan. hsuzuki@sapmed.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180801
PL  - England
TA  - Cell Death Dis
JT  - Cell death & disease
JID - 101524092
RN  - 0 (DLEU1 lncRNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/metabolism/mortality/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Mice
MH  - Mice, Nude
MH  - Mouth Neoplasms/metabolism/mortality/*pathology
MH  - RNA Interference
MH  - RNA, Long Noncoding/antagonists & inhibitors/genetics/*metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Tumor Suppressor Proteins/antagonists & inhibitors/genetics/*metabolism
PMC - PMC6070574
COIS- The authors declare that they have no conflict of interest.
EDAT- 2018/08/03 06:00
MHDA- 2019/12/04 06:00
PMCR- 2018/08/01
CRDT- 2018/08/03 06:00
PHST- 2018/03/02 00:00 [received]
PHST- 2018/07/18 00:00 [accepted]
PHST- 2018/07/18 00:00 [revised]
PHST- 2018/08/03 06:00 [entrez]
PHST- 2018/08/03 06:00 [pubmed]
PHST- 2019/12/04 06:00 [medline]
PHST- 2018/08/01 00:00 [pmc-release]
AID - 10.1038/s41419-018-0893-2 [pii]
AID - 893 [pii]
AID - 10.1038/s41419-018-0893-2 [doi]
PST - epublish
SO  - Cell Death Dis. 2018 Aug 1;9(8):826. doi: 10.1038/s41419-018-0893-2.