PMID- 30069594
OWN - NLM
STAT- MEDLINE
DCOM- 20180920
LR  - 20220321
IS  - 1432-2242 (Electronic)
IS  - 0040-5752 (Print)
IS  - 0040-5752 (Linking)
VI  - 131
IP  - 10
DP  - 2018 Oct
TI  - The Agropyron cristatum karyotype, chromosome structure and cross-genome 
      homoeology as revealed by fluorescence in situ hybridization with tandem repeats 
      and wheat single-gene probes.
PG  - 2213-2227
LID - 10.1007/s00122-018-3148-9 [doi]
AB  - Fluorescence in situ hybridization with probes for 45 cDNAs and five tandem 
      repeats revealed homoeologous relationships of Agropyron cristatum with wheat. 
      The results will contribute to alien gene introgression in wheat improvement. 
      Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat 
      and a promising source of novel genes for wheat improvement. To date, 
      identification of A. cristatum chromosomes has not been possible, and its 
      molecular karyotype has not been available. Furthermore, homoeologous 
      relationship between the genomes of A. cristatum and wheat has not been 
      determined. To develop chromosome-specific landmarks, A. cristatum genomic DNA 
      was sequenced, and new tandem repeats were discovered. Their distribution on 
      mitotic chromosomes was studied by fluorescence in situ hybridization (FISH), 
      which revealed specific patterns for five repeats in addition to 5S and 45S 
      ribosomal DNA and rye subtelomeric repeats pSc119.2 and pSc200. FISH with one 
      tandem repeat together with 45S rDNA enabled identification of all A. cristatum 
      chromosomes. To analyze the structure and cross-species homoeology of A. 
      cristatum chromosomes with wheat, probes for 45 mapped wheat cDNAs covering all 
      seven chromosome groups were localized by FISH. Thirty-four cDNAs hybridized to 
      homoeologous chromosomes of A. cristatum, nine hybridized to homoeologous and 
      non-homoeologous chromosomes, and two hybridized to unique positions on 
      non-homoeologous chromosomes. FISH using single-gene probes revealed that the 
      wheat-A. cristatum collinearity was distorted, and important structural 
      rearrangements were observed for chromosomes 2P, 4P, 5P, 6P and 7P. Chromosomal 
      inversions were found for pericentric region of 4P and whole chromosome arm 6PL. 
      Furthermore, reciprocal translocations between 2PS and 4PL were detected. These 
      results provide new insights into the genome evolution within Triticeae and will 
      facilitate the use of crested wheatgrass in alien gene introgression into wheat.
FAU - Said, Mahmoud
AU  - Said M
AUID- ORCID: 0000-0002-6169-8655
AD  - Institute of Experimental Botany, Center of the Region Hana for Biotechnological 
      and Agricultural Research, Slechtitelu 31, 78371, Olomouc, Czech Republic.
AD  - Field Crops Research Institute, Agricultural Research Centre, 9 Gamma Street, 
      Giza, Cairo, 12619, Egypt.
FAU - Hribova, Eva
AU  - Hribova E
AD  - Institute of Experimental Botany, Center of the Region Hana for Biotechnological 
      and Agricultural Research, Slechtitelu 31, 78371, Olomouc, Czech Republic.
FAU - Danilova, Tatiana V
AU  - Danilova TV
AD  - Wheat Genetics Resource Center, Kansas State University, 1712 Claflin Road, 4024 
      Throckmorton PSC, Manhattan, KS, 66506, USA.
FAU - Karafiatova, Miroslava
AU  - Karafiatova M
AD  - Institute of Experimental Botany, Center of the Region Hana for Biotechnological 
      and Agricultural Research, Slechtitelu 31, 78371, Olomouc, Czech Republic.
FAU - Cizkova, Jana
AU  - Cizkova J
AD  - Institute of Experimental Botany, Center of the Region Hana for Biotechnological 
      and Agricultural Research, Slechtitelu 31, 78371, Olomouc, Czech Republic.
FAU - Friebe, Bernd
AU  - Friebe B
AD  - Wheat Genetics Resource Center, Kansas State University, 1712 Claflin Road, 4024 
      Throckmorton PSC, Manhattan, KS, 66506, USA.
FAU - Dolezel, Jaroslav
AU  - Dolezel J
AUID- ORCID: 0000-0002-6263-0492
AD  - Institute of Experimental Botany, Center of the Region Hana for Biotechnological 
      and Agricultural Research, Slechtitelu 31, 78371, Olomouc, Czech Republic.
FAU - Gill, Bikram S
AU  - Gill BS
AD  - Wheat Genetics Resource Center, Kansas State University, 1712 Claflin Road, 4024 
      Throckmorton PSC, Manhattan, KS, 66506, USA.
FAU - Vrana, Jan
AU  - Vrana J
AUID- ORCID: 0000-0001-6171-8516
AD  - Institute of Experimental Botany, Center of the Region Hana for Biotechnological 
      and Agricultural Research, Slechtitelu 31, 78371, Olomouc, Czech Republic. 
      vrana@ueb.cas.cz.
LA  - eng
GR  - 16-16992S/Grantova Agentura Ceske Republiky (CZ)/
GR  - LO1204/Ministerstvo Skolstvi, Mladeze a Telovychovy/
PT  - Journal Article
DEP - 20180801
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
RN  - 0 (DNA Probes)
SB  - IM
MH  - Agropyron/*genetics
MH  - *Chromosomes, Plant
MH  - DNA Probes
MH  - Diploidy
MH  - In Situ Hybridization, Fluorescence
MH  - *Karyotype
MH  - *Tandem Repeat Sequences
MH  - Translocation, Genetic
MH  - Triticum/genetics
PMC - PMC6154037
COIS- All the authors declare that they have no conflict of interest.
EDAT- 2018/08/03 06:00
MHDA- 2018/09/21 06:00
PMCR- 2018/08/01
CRDT- 2018/08/03 06:00
PHST- 2018/01/08 00:00 [received]
PHST- 2018/07/23 00:00 [accepted]
PHST- 2018/08/03 06:00 [pubmed]
PHST- 2018/09/21 06:00 [medline]
PHST- 2018/08/03 06:00 [entrez]
PHST- 2018/08/01 00:00 [pmc-release]
AID - 10.1007/s00122-018-3148-9 [pii]
AID - 3148 [pii]
AID - 10.1007/s00122-018-3148-9 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2018 Oct;131(10):2213-2227. doi: 10.1007/s00122-018-3148-9. 
      Epub 2018 Aug 1.