PMID- 30072872
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 12
DP  - 2018
TI  - Imaging Calcium in Hippocampal Presynaptic Terminals With a Ratiometric Calcium 
      Sensor in a Novel Transgenic Mouse.
PG  - 209
LID - 10.3389/fncel.2018.00209 [doi]
LID - 209
AB  - Genetically encoded calcium indicators (GECIs) have gained widespread use for 
      measurement of neuronal activity but their low expression levels in transgenic 
      mice tend to limit sensitivity. We have developed a transgenic mouse line (SyG37) 
      that expresses a ratiometric calcium sensor, SyGCaMP2-mCherry, that is expressed 
      throughout the brain but targeted to presynaptic terminals. Within the CA1 and 
      CA3 regions of hippocampus of male and female mice, SyGaMP2 fluorescence responds 
      linearly up to 10 electrical stimuli at frequencies up to 100 Hz and it can 
      detect responses to a single stimulus. Responses in single boutons can be 
      measured using multiphoton microscopy. The ensemble amplitude of SyGCaMP2 
      responses is a function of the number of stimuli applied and the number of 
      contributing boutons. The peak responses and initial rates of calcium influx in 
      single boutons in CA1 and CA3 were similar but the rate of calcium clearance from 
      CA3 boutons after stimulation was significantly faster. In CA1, DNQX reduced 
      SyGCaMP2 responses to Schaffer collateral stimulation to 86% of baseline 
      indicating that 14% of the total response originated from presynaptic terminals 
      of neurones synaptically driven via AMPA receptors. Theta burst stimulation 
      induced long-term potentiation (LTP) of both SyGCaMP2 and fEPSP responses in both 
      young and 18-month-old mice. The proportion of postsynaptically connected 
      terminals increased significantly to 76% of the total after LTP induction. The 
      SyG37 mouse allows stable optical detection of synaptic activation and 
      connectivity at the single bouton level and can be used to characterize the 
      contributions of presynaptic calcium to synaptic transmission and plasticity.
FAU - Al-Osta, Ibrahim
AU  - Al-Osta I
AD  - Department of Neuroscience, Psychology and Behaviour, University of Leicester, 
      Leicester, United Kingdom.
FAU - Mucha, Mariusz
AU  - Mucha M
AD  - Department of Neuroscience, Psychology and Behaviour, University of Leicester, 
      Leicester, United Kingdom.
FAU - Pereda, Daniel
AU  - Pereda D
AD  - Department of Neuroscience, Psychology and Behaviour, University of Leicester, 
      Leicester, United Kingdom.
FAU - Pique-Gili, Marta
AU  - Pique-Gili M
AD  - Department of Neuroscience, Psychology and Behaviour, University of Leicester, 
      Leicester, United Kingdom.
FAU - Okorocha, Albert E
AU  - Okorocha AE
AD  - Department of Neuroscience, Psychology and Behaviour, University of Leicester, 
      Leicester, United Kingdom.
FAU - Thomas, Roisin
AU  - Thomas R
AD  - Department of Neuroscience, Psychology and Behaviour, University of Leicester, 
      Leicester, United Kingdom.
FAU - Hartell, Nicholas A
AU  - Hartell NA
AD  - Department of Neuroscience, Psychology and Behaviour, University of Leicester, 
      Leicester, United Kingdom.
LA  - eng
PT  - Journal Article
DEP - 20180719
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
EIN - Front Cell Neurosci. 2019 May 15;13:194. PMID: 31156390
PMC - PMC6060260
OTO - NOTNLM
OT  - CA1
OT  - CA3
OT  - calcium imaging
OT  - hippocampus
OT  - long-term potentiation
OT  - presynaptic terminals
OT  - transgenic mice
EDAT- 2018/08/04 06:00
MHDA- 2018/08/04 06:01
PMCR- 2018/01/01
CRDT- 2018/08/04 06:00
PHST- 2018/02/11 00:00 [received]
PHST- 2018/06/26 00:00 [accepted]
PHST- 2018/08/04 06:00 [entrez]
PHST- 2018/08/04 06:00 [pubmed]
PHST- 2018/08/04 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2018.00209 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2018 Jul 19;12:209. doi: 10.3389/fncel.2018.00209. 
      eCollection 2018.