PMID- 30074319
OWN - NLM
STAT- MEDLINE
DCOM- 20180904
LR  - 20200923
IS  - 0390-6663 (Print)
IS  - 0390-6663 (Linking)
VI  - 43
IP  - 5
DP  - 2016
TI  - Application of fluorescence in situ hybridization (FISH) as a tool to aid 
      cytogenetics in 1,409 fetal samples.
PG  - 685-690
AB  - AIM: To evaluate the technical application of fluorescence in situ hybridization 
      (FISH) as a support to classical cytogenetic in numerical chromosomal 
      aneuploidies studies in samples of amniotic fluid, chorionic villus, and fetal 
      loss. MATERIALS AND METHODS: The authois performed cytogenetic analyses in 1,409 
      patients (678 amniocentesis, 512 chorionic villus samples, and 219 spontaneous 
      abortions) during one year. FISH molecular study aided traditional cytogenetic in 
      90 cases. These cases were indicated based on the diagnostic hypothesis of each 
      patient or when no cellular growth was obtained. The authors standardized the 
      FISH in discoloured slides. RESULTS: They had 85% positive FISH in amniotic 
      fluid, 70% in chorionic villus, and 90% in abortion material using 13, 18, 21 X 
      and Y centromeric probes. It showed 12% of altered FISH in amniotic fluid (100% 
      trisomies), 10% in chorionic'villus (50% trisomy and 50% X - monosomy), and 22% 
      in abortion material (50% trisomy, 25% X-monosomy, and 25% triploidy). FISH and 
      cytogenetic analysis confirmed the results. CONCLUSION: This technique 
      revolutionized clinical and research applications of cytogenetics. In this 
      particular paper, FISH was a valuable and reliable technique to promptly identify 
      rapid detection of aneuploidies in interphase cells, metaphase spread and 
      paraffin-embedded samples. It is hoped that, in the future, the economic 
      viability of array CGH and FISH, with the decreasing cost of testing and their 
      genomics advantages can be incorporated as routine and customized in the approach 
      of prenatal diagnosis.
FAU - de Moraex-Malinverni, A C
AU  - de Moraex-Malinverni AC
FAU - Patricio, F R S
AU  - Patricio FRS
FAU - Oshima, C T F
AU  - Oshima CTF
FAU - Moron, A F
AU  - Moron AF
FAU - da Silva, I D C G
AU  - da Silva IDCG
FAU - de Souza, M M
AU  - de Souza MM
LA  - eng
PT  - Journal Article
PL  - Singapore
TA  - Clin Exp Obstet Gynecol
JT  - Clinical and experimental obstetrics & gynecology
JID - 7802110
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Cytogenetic Analysis/*methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
EDAT- 2016/01/01 00:00
MHDA- 2016/01/01 00:01
CRDT- 2018/08/04 06:00
PHST- 2018/08/04 06:00 [entrez]
PHST- 2016/01/01 00:00 [pubmed]
PHST- 2016/01/01 00:01 [medline]
PST - ppublish
SO  - Clin Exp Obstet Gynecol. 2016;43(5):685-690.