PMID- 30077100
OWN - NLM
STAT- MEDLINE
DCOM- 20181009
LR  - 20181009
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1095
DP  - 2018 Sep 15
TI  - Quantitative analysis of underivatized amino acids in the sub- to 
      several-nanomolar range by ion-pair HPLC using a corona-charged aerosol detector 
      (HPLC-CAD).
PG  - 191-197
LID - S1570-0232(18)30659-7 [pii]
LID - 10.1016/j.jchromb.2018.07.033 [doi]
AB  - The separation and quantification of underivatized naturally occurring amino 
      acids (AAs) by high-performance liquid chromatography (HPLC) is advantageous for 
      reducing preparation time, running costs, and analytical errors, and is 
      compatible with the isolation of intact AAs. This study establishes and validates 
      an analytical method for the separation and quantification of underivatized AAs 
      and taurine (Tau) in the sub- to several-nanomolar range (ca. 0.1-1.6 nmol) by 
      optimizing ion-pair HPLC coupled to a corona charged aerosol detector (corona 
      CAD). Chromatographic separation of 19 AAs and Tau was achieved using a porous 
      graphite carbon (PGC) column and nonafluoropentanoic acid (NFPA) as a volatile 
      ion-pair reagent. The response of the corona CAD to the AAs was highly dependent 
      on the eluate composition, whereas these response factors were similar for AAs in 
      eluate with similar compositions. Regression curves and coefficients (r(2)) 
      >0.998 were obtained for plots of injection amount versus peak area, except for 
      Arg which co-eluted with a background peak. On the other hand, all plots of 
      injection amount versus peak height regressed to curves with r(2) > 0.997. Repeat 
      quantification based on peak area showed lower relative standard deviations 
      (RSDs) (typically better than 5%) than those based on peak height. The present 
      method is useful for quantifying AAs from proteins and Tau in the sub- to 
      several-nanomolar range without derivatization, and constant repeatability can be 
      maintained by quantification using peak areas.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Furota, Satoshi
AU  - Furota S
AD  - Department of Biogeochemistry, Japan Agency for Marine-Earth Science and 
      Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. Electronic 
      address: furota@jamstec.go.jp.
FAU - Ogawa, Nanako O
AU  - Ogawa NO
AD  - Department of Biogeochemistry, Japan Agency for Marine-Earth Science and 
      Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
FAU - Takano, Yoshinori
AU  - Takano Y
AD  - Department of Biogeochemistry, Japan Agency for Marine-Earth Science and 
      Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
FAU - Yoshimura, Toshihiro
AU  - Yoshimura T
AD  - Department of Biogeochemistry, Japan Agency for Marine-Earth Science and 
      Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
FAU - Ohkouchi, Naohiko
AU  - Ohkouchi N
AD  - Department of Biogeochemistry, Japan Agency for Marine-Earth Science and 
      Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180725
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Aerosols)
RN  - 0 (Amino Acids)
SB  - IM
MH  - Aerosols/analysis/chemistry
MH  - Amino Acids/*analysis/*chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Limit of Detection
MH  - Linear Models
MH  - Reproducibility of Results
EDAT- 2018/08/05 06:00
MHDA- 2018/10/10 06:00
CRDT- 2018/08/05 06:00
PHST- 2018/04/26 00:00 [received]
PHST- 2018/07/24 00:00 [revised]
PHST- 2018/07/25 00:00 [accepted]
PHST- 2018/08/05 06:00 [pubmed]
PHST- 2018/10/10 06:00 [medline]
PHST- 2018/08/05 06:00 [entrez]
AID - S1570-0232(18)30659-7 [pii]
AID - 10.1016/j.jchromb.2018.07.033 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Sep 15;1095:191-197. doi: 
      10.1016/j.jchromb.2018.07.033. Epub 2018 Jul 25.