PMID- 30077662
OWN - NLM
STAT- MEDLINE
DCOM- 20190325
LR  - 20190325
IS  - 1872-7913 (Electronic)
IS  - 0924-8579 (Linking)
VI  - 52
IP  - 6
DP  - 2018 Dec
TI  - Detection and characterization of methicillin-resistant and susceptible 
      coagulase-negative staphylococci in milk from cows with clinical mastitis in 
      Tunisia.
PG  - 930-935
LID - S0924-8579(18)30225-5 [pii]
LID - 10.1016/j.ijantimicag.2018.07.026 [doi]
AB  - OBJECTIVES: This study investigated prevalence of methicillin-resistant (MR) and 
      methicillin-susceptible (MS) coagulase-negative staphylococci (CNS) and the 
      implicated mechanisms of resistance and virulence in milk of mastitis cows. In 
      addition, the presence of SCCmec type was analyzed in MR Staphylococcus 
      epidermidis (MRSE). RESULTS: Three hundred milk samples from cows with clinical 
      mastitis were obtained from 30 dairy farms in different regions of Tunisia. 
      Sixty-eight of the 300 tested samples contained CNS strains. Various CNS species 
      were identified, with Staphylococcus xylosus being the most frequently found 
      (40%) followed by Staphylococcus warneri (12%). The mecA gene was present in 14 
      of 20 MR-CNS isolates. All of them were lacking the mecC gene. The SCCmecIVa was 
      identified in four MRSE isolates. Most of CNS isolates showed penicillin 
      resistance (70.6%) and 58.3% of them carried the blaZ gene. MR-CNS isolates 
      (n = 20) showed resistance to erythromycin, tetracycline and 
      trimethoprim-sulfametoxazole harboring different resistance genes such us erm(B), 
      erm(T), erm(C), mph(C) or msr(A), tet(K) and dfr(A). However, a lower percentage 
      of resistance was observed among 48 MS-CNS isolates: erythromycin (8.3%), 
      tetracycline (6.2%), streptomycin (6.2%), clindamycin (6.2%), and 
      trimethoprim-sulfametoxazole (2%). The Inu(B) gene was detected in one 
      Staphylococcus xylosus strain that showed clindamycin resistance. The virulence 
      gene tsst-1 was observed in one MR-CNS strain. DISCUSSION: Coagulase-negative 
      staphylococci containing a diversity of antimicrobial resistance genes are 
      frequently detected in milk of mastitis cows. This fact emphasizes the importance 
      of identifying CNS when an intramammary infection is present because of the 
      potential risk of lateral transfer of resistant genes among staphylococcal 
      species and other pathogenic bacteria.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Klibi, Amira
AU  - Klibi A
AD  - Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and 
      Biotechnology Developement, Pasteur Institute of Tunis, BP 74, 13 Place Pasteur, 
      Belvedere, 1002 Tunis, Tunisia. Universite de Tunis El Manar, 2092 Tunis, 
      Tunisie.
FAU - Maaroufi, Abderrazek
AU  - Maaroufi A
AD  - Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and 
      Biotechnology Developement, Pasteur Institute of Tunis, BP 74, 13 Place Pasteur, 
      Belvedere, 1002 Tunis, Tunisia. Universite de Tunis El Manar, 2092 Tunis, 
      Tunisie.
FAU - Torres, Carmen
AU  - Torres C
AD  - Area of Biochemistry and Molecular Biology, University of La Rioja, Logrono, 
      Spain.
FAU - Jouini, Ahlem
AU  - Jouini A
AD  - Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and 
      Biotechnology Developement, Pasteur Institute of Tunis, BP 74, 13 Place Pasteur, 
      Belvedere, 1002 Tunis, Tunisia. Universite de Tunis El Manar, 2092 Tunis, 
      Tunisie. Electronic address: ahlem.jouini@pasteur.rns.tn.
LA  - eng
PT  - Journal Article
DEP - 20180803
PL  - Netherlands
TA  - Int J Antimicrob Agents
JT  - International journal of antimicrobial agents
JID - 9111860
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Coagulase)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Cattle
MH  - Coagulase/*analysis
MH  - Genes, Bacterial
MH  - Genotype
MH  - Mastitis, Bovine/epidemiology/*microbiology
MH  - *Methicillin Resistance
MH  - Microbial Sensitivity Tests
MH  - Milk/*microbiology
MH  - Prevalence
MH  - Staphylococcal Infections/epidemiology/microbiology/*veterinary
MH  - Staphylococcus/*classification/drug effects/genetics/*isolation & purification
MH  - Tunisia/epidemiology
MH  - Virulence Factors/analysis/genetics
OTO - NOTNLM
OT  - Bovine mastitis.
OT  - Methicillin-resistant coagulase-negative staphylococci (MR-CNS)
OT  - Methicillin-susceptible coagulase-negative staphylococci (MS-CNS)
OT  - Resistance and virulence genes
OT  - SCCmec methicillin-resistant Staphylococcus epidermidis (MRSE)
OT  - mecA gene
EDAT- 2018/08/06 06:00
MHDA- 2019/03/26 06:00
CRDT- 2018/08/06 06:00
PHST- 2018/03/05 00:00 [received]
PHST- 2018/06/01 00:00 [revised]
PHST- 2018/07/29 00:00 [accepted]
PHST- 2018/08/06 06:00 [pubmed]
PHST- 2019/03/26 06:00 [medline]
PHST- 2018/08/06 06:00 [entrez]
AID - S0924-8579(18)30225-5 [pii]
AID - 10.1016/j.ijantimicag.2018.07.026 [doi]
PST - ppublish
SO  - Int J Antimicrob Agents. 2018 Dec;52(6):930-935. doi: 
      10.1016/j.ijantimicag.2018.07.026. Epub 2018 Aug 3.