PMID- 30077872
OWN - NLM
STAT- MEDLINE
DCOM- 20190207
LR  - 20190215
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 118
DP  - 2018 Oct 30
TI  - Label-free leukocyte sorting and impedance-based profiling for diabetes testing.
PG  - 195-203
LID - S0956-5663(18)30557-8 [pii]
LID - 10.1016/j.bios.2018.07.052 [doi]
AB  - Circulating leukocytes comprise of approximately 1% of all blood cells and 
      efficient enrichment of these cells from whole blood is critical for 
      understanding cellular heterogeneity and biological significance in health and 
      diseases. In this work, we report a novel microfluidic strategy for rapid (< 1 h) 
      label-free leukocyte sorting and impedance-based profiling to determine cell 
      activation in type 2 diabetes mellitus (T2DM) using whole blood. Leukocytes were 
      first size-fractionated into different subtypes (neutrophils, monocytes, 
      lymphocytes) using an inertial spiral sorter prior to single-cell impedance 
      measurement in a microfluidic device with coplanar electrode design. Significant 
      changes in membrane dielectric properties (size and opacity) were detected 
      between healthy and activated leukocytes (TNF-alpha/LPS stimulated), during monocyte 
      differentiation and among different monocyte subsets (classical, intermediate, 
      non-classical). As proof-of-concept for diabetes testing, neutrophil/monocyte 
      dielectric properties in T2DM subjects (n = 8) were quantified which were 
      associated with cardiovascular risk factors including lipid levels, C-reactive 
      protein (CRP) and vascular functions (LnRHI) (P < 0.05) were observed. Overall, 
      these results clearly showed that T2DM subjects have pro-inflammatory leukocyte 
      phenotypes and suggest leukocyte impedance signature as a novel surrogate 
      biomarker for inflammation.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Petchakup, Chayakorn
AU  - Petchakup C
AD  - School of Mechanical and Aerospace Engineering, Nanyang Technological University, 
      50 Nanyang Avenue, Block N3, Singapore 639798, Singapore.
FAU - Tay, Hui Min
AU  - Tay HM
AD  - Lee Kong Chian School of Medicine, Nanyang Technological University, 11 Mandalay 
      Road, Clinical Sciences Building Level 11, Singapore 308232, Singapore.
FAU - Yeap, Wei Hseun
AU  - Yeap WH
AD  - Singapore Immunology Network (SIgN), Agency for Science, Technology and Research 
      (A⁎STAR), 8A Biomedical Grove, Immunos, Singapore 138648, Singapore.
FAU - Dalan, Rinkoo
AU  - Dalan R
AD  - Endocrinology Department, Tan Tock Seng Hospital, Singapore 308433, Singapore.
FAU - Wong, Siew Cheng
AU  - Wong SC
AD  - Singapore Immunology Network (SIgN), Agency for Science, Technology and Research 
      (A⁎STAR), 8A Biomedical Grove, Immunos, Singapore 138648, Singapore; Department 
      of Microbiology and Immunology, Yong Loo Lin School of Medicine, National 
      University of Singapore, Singapore 117545, Singapore.
FAU - Li, King Ho Holden
AU  - Li KHH
AD  - School of Mechanical and Aerospace Engineering, Nanyang Technological University, 
      50 Nanyang Avenue, Block N3, Singapore 639798, Singapore. Electronic address: 
      holdenli@ntu.edu.sg.
FAU - Hou, Han Wei
AU  - Hou HW
AD  - School of Mechanical and Aerospace Engineering, Nanyang Technological University, 
      50 Nanyang Avenue, Block N3, Singapore 639798, Singapore; Lee Kong Chian School 
      of Medicine, Nanyang Technological University, 11 Mandalay Road, Clinical 
      Sciences Building Level 11, Singapore 308232, Singapore. Electronic address: 
      hwhou@ntu.edu.sg.
LA  - eng
PT  - Journal Article
DEP - 20180726
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
SB  - IM
MH  - Biosensing Techniques/instrumentation/*methods
MH  - Cell Separation
MH  - Diabetes Mellitus, Type 2/*diagnosis
MH  - *Electric Impedance
MH  - Humans
MH  - Leukocytes/cytology
MH  - *Microfluidics
OTO - NOTNLM
OT  - Dean flow fractionation
OT  - Diabetes mellitus
OT  - Impedance cytometry
OT  - Leukocyte phenotyping
EDAT- 2018/08/06 06:00
MHDA- 2019/02/08 06:00
CRDT- 2018/08/06 06:00
PHST- 2018/05/08 00:00 [received]
PHST- 2018/07/18 00:00 [revised]
PHST- 2018/07/25 00:00 [accepted]
PHST- 2018/08/06 06:00 [pubmed]
PHST- 2019/02/08 06:00 [medline]
PHST- 2018/08/06 06:00 [entrez]
AID - S0956-5663(18)30557-8 [pii]
AID - 10.1016/j.bios.2018.07.052 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2018 Oct 30;118:195-203. doi: 10.1016/j.bios.2018.07.052. 
      Epub 2018 Jul 26.