PMID- 30078282
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 98
IP  - 28
DP  - 2018 Jul 24
TI  - [Therapeutic effect of human umbilical cord mesenchymal stem cells on airway 
      remodeling in an asthma model of rat and its possible mechanism].
PG  - 2258-2263
LID - 10.3760/cma.j.issn.0376-2491.2018.28.010 [doi]
AB  - Objective: To observe the effect of human umbilical cord mesenchymal stem cells 
      (hUC-MSCs) on airway remodeling in asthma model of rat and its possible 
      mechanism. Methods: hUC-MSCs were isolated and cultured, and surface markers of 
      hUC-MSCs were identified by flow cytometry. Forty Wistar male rats were divided 
      into 4 groups: Control, Model, MSCs, Budesonide. The rats of Control group were 
      sensitized and challenged by normal saline. The rats of Model, MSCs and 
      Budesonide group were sensitized and challenged by ovalbumin (OVA) for 8 weeks. 
      The MSCs group rats were given a tail vein injection of MSCs 0.2 ml (1x10(6) /ml) 
      on days 35, 45, and 55 half an hour befor each OVA exposure. The Budesonide group 
      rats were given aerosol inhalation of budesonide 2 mg two hours before each OVA 
      exposure. Specimens were collected within 24 hours after the last OVA challenge. 
      The parameters of airway morphological changes and the degree of airway 
      remodeling were analyzed using Image-pro plus computer graphics. The levels of 
      transformation growth factor (TGF) -beta(1) in bronchoalveolar lavage fluid (BALF) 
      and serum were detected by enzyme linked immunosorbent assay (ELISA). The 
      expressions of E-cadherin, alpha-smooth muscle actin (alpha-SMA) and fibronectin (Fn) 
      were measured by immunohistochemistry. Results: The thickness of airway wall and 
      smooth muscle of Model, MSCs and Budesonide group rats were significantly thicker 
      than Control group. The levels of TGF-beta(1) in both BALF and serum of Model, MSCs 
      and Budesonide group rats were significantly higher than Control group. The 
      expression of E-cadherin of Model, MSCs and Budesonide group rats was 
      significantly lower than Control group, while the expression of alpha-SMA and Fn were 
      significantly higher. The thickness of airway wall and smooth muscle of MSCs and 
      Budesonide group rats were significantly lower than Model group[(38.40+/-2.50, 
      45.34+/-0.33) vs (80.18+/-1.75) mum and (15.71+/-0.89, 18.57+/-0.67) vs (40.97+/-0.90) mum]. 
      The levels of TGF-beta(1) in both BALF and serum of MSCs and Budesonide group were 
      significantly lower than Model group[(3.53+/-0.43, 3.11+/-0.05) vs (20.88+/-0.37) mug/L 
      and (31.07+/-0.89, 31.12+/-0.50) vs (70.58+/-0.39)mug/L](all P<0.01). The expressions of 
      alpha-SMA, Fn of MSCs and Budesonide group rats were significantly lower than Model 
      group[(0.438+/-0.057, 0.445+/-0.027) vs (0.521+/-0.030) and (0.459+/-0.041, 0.458+/-0.029) 
      vs (0.527+/-0.022)], While the expression of E-cadherin was significantly 
      higher[(0.308+/-0.023, 0.296+/-0.010) vs (0.256+/-0.087)](all P<0.01). Conclusion: MSCs 
      could alleviate asthmatic airway remodeling, the mechanism of which may be 
      associated with the inhibition of TGF-beta(1) induced epithelial-mesenchymal 
      transition.
FAU - Chang, Q
AU  - Chang Q
AD  - Department of Respiratory and Critical Care Medicine, the Second Hospital of 
      Shanxi Medical School, Taiyuan 030001, China.
FAU - Tian, X L
AU  - Tian XL
FAU - Huo, R J
AU  - Huo RJ
FAU - Liu, D
AU  - Liu D
FAU - Chen, J Y
AU  - Chen JY
FAU - Wang, X
AU  - Wang X
FAU - Tian, X R
AU  - Tian XR
LA  - chi
GR  - 2016[366]/Foundation for Returnees of Human Resources and Social Security of 
      China/
GR  - 2017-8/Foundation for Returnees of Shanxi Province of China/
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
SB  - IM
MH  - Airway Remodeling
MH  - Animals
MH  - Asthma
MH  - Disease Models, Animal
MH  - Humans
MH  - Male
MH  - *Mesenchymal Stem Cells
MH  - Rats
MH  - Rats, Wistar
MH  - Umbilical Cord
OTO - NOTNLM
OT  - Airway remodeling
OT  - Asthma
OT  - Epithelial-mesenchymal transition
OT  - Mesenchymal stem cells
EDAT- 2018/08/07 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/08/06 06:00
PHST- 2018/08/06 06:00 [entrez]
PHST- 2018/08/07 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
AID - 10.3760/cma.j.issn.0376-2491.2018.28.010 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2018 Jul 24;98(28):2258-2263. doi: 
      10.3760/cma.j.issn.0376-2491.2018.28.010.