PMID- 30078834
OWN - NLM
STAT- MEDLINE
DCOM- 20180816
LR  - 20180816
IS  - 1880-3989 (Electronic)
IS  - 0388-1350 (Linking)
VI  - 43
IP  - 8
DP  - 2018
TI  - Polyhexamethylene guanidine phosphate induces IL-6 and TNF-alpha expression through 
      JNK-dependent pathway in human lung epithelial cells.
PG  - 485-492
LID - 10.2131/jts.43.485 [doi]
AB  - Polyhexamethylene guanidine phosphate (PHMG) is an antimicrobial biocide that 
      causes severe lung injury accompanied with inflammation and subsequent fibrosis. 
      Cytokines mediate the inflammatory response, leading to fibrosis in injured 
      tissues. PHMG is known to induce the expression of various cytokines in vitro and 
      in vivo. In the present study, we investigated the involvement of three MAPK 
      subfamilies (JNK, p38 MAPK, and ERK) in PHMG-induced cytokine expression in A549 
      human lung epithelial cells. Our in vivo and in vitro data indicated that PHMG 
      induced an increase in mRNA expression of IL-6 and TNF-alpha, and enhanced the 
      phosphorylation of JNK, p38 MAPK, and ERK. Further, we investigated the 
      involvement of MAPKs in PHMG-induced mRNA expression of IL-6 and TNF-alpha using JNK, 
      p38 MAPK, and ERK inhibitors in A549 cells. Pre-treatment with the JNK inhibitor 
      but not the p38 MAPK or ERK inhibitor, significantly attenuated the PHMG-induced 
      mRNA expression of IL-6 and TNF-alpha. These results suggest that the activation of 
      JNK is involved at least partially in the induction of IL-6 or TNF-alpha expression 
      by PHMG in A549 cells.
FAU - Kim, Min-Seok
AU  - Kim MS
AD  - National Center for Efficacy Evaluation of Respiratory Disease Product, Korea 
      Institute of Toxicology, Korea.
FAU - Han, Jin-Young
AU  - Han JY
AD  - National Center for Efficacy Evaluation of Respiratory Disease Product, Korea 
      Institute of Toxicology, Korea.
AD  - Department of human and environmental toxicology, University of Science & 
      Technology, Korea.
FAU - Kim, Sung-Hwan
AU  - Kim SH
AD  - National Center for Efficacy Evaluation of Respiratory Disease Product, Korea 
      Institute of Toxicology, Korea.
AD  - Department of human and environmental toxicology, University of Science & 
      Technology, Korea.
FAU - Kim, Hyung-Young
AU  - Kim HY
AD  - National Center for Efficacy Evaluation of Respiratory Disease Product, Korea 
      Institute of Toxicology, Korea.
FAU - Jeon, Doin
AU  - Jeon D
AD  - National Center for Efficacy Evaluation of Respiratory Disease Product, Korea 
      Institute of Toxicology, Korea.
FAU - Lee, Kyuhong
AU  - Lee K
AD  - National Center for Efficacy Evaluation of Respiratory Disease Product, Korea 
      Institute of Toxicology, Korea.
AD  - Department of human and environmental toxicology, University of Science & 
      Technology, Korea.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Toxicol Sci
JT  - The Journal of toxicological sciences
JID - 7805798
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Guanidines)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31961-54-3 (polyhexamethyleneguanidine)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - A549 Cells
MH  - Animals
MH  - Anti-Infective Agents/*adverse effects
MH  - Epithelial Cells/*metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Gene Expression/drug effects
MH  - Guanidines/*adverse effects
MH  - Humans
MH  - Interleukin-6/genetics/*metabolism
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - Lung/*metabolism
MH  - MAP Kinase Signaling System/*drug effects
MH  - Mice, Inbred C57BL
MH  - RNA, Messenger/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/genetics/*metabolism
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - Cytokine
OT  - Inflammation
OT  - MAPKs
OT  - PHMG
OT  - Pulmonary fibrosis
EDAT- 2018/08/07 06:00
MHDA- 2018/08/17 06:00
CRDT- 2018/08/07 06:00
PHST- 2018/08/07 06:00 [entrez]
PHST- 2018/08/07 06:00 [pubmed]
PHST- 2018/08/17 06:00 [medline]
AID - 10.2131/jts.43.485 [doi]
PST - ppublish
SO  - J Toxicol Sci. 2018;43(8):485-492. doi: 10.2131/jts.43.485.