PMID- 30080115
OWN - NLM
STAT- MEDLINE
DCOM- 20190429
LR  - 20190429
IS  - 1744-8387 (Electronic)
IS  - 1478-9450 (Linking)
VI  - 15
IP  - 8
DP  - 2018 Aug
TI  - Employing proteomics in the study of antigen presentation: an update.
PG  - 637-645
LID - 10.1080/14789450.2018.1509000 [doi]
AB  - Our immune system discriminates self from non-self by examining the peptide cargo 
      of human leukocyte antigen (HLA) molecules displayed on the cell surface. 
      Successful recognition of HLA-bound non-self peptides can induce T cell responses 
      leading to, for example, the destruction of infected cells. Today, largely due to 
      advances in technology, we have an unprecedented capability to identify the 
      nature of these presented peptides and unravel the true complexity of antigen 
      presentation. Areas covered: In addition to conventional linear peptides, HLA 
      molecules also present post-translationally modified sequences comprising a 
      wealth of chemical and structural modifications, including a novel class of 
      noncontiguous spliced peptides. This review focuses on these emerging themes in 
      antigen presentation and how mass spectrometry in particular has contributed to a 
      new view of the antigenic landscape that is presented to the immune system. 
      Expert Commentary: Advances in the sensitivity of mass spectrometers and use of 
      hybrid fragmentation technologies will provide more information-rich spectra of 
      HLA bound peptides leading to more definitive identification of T cell epitopes. 
      Coupled with improvements in sample preparation and new informatics workflows, 
      studies will access novel classes of peptide antigen and allow interrogation of 
      rare and clinically relevant samples.
FAU - Ramarathinam, Sri H
AU  - Ramarathinam SH
AD  - a Department of Biochemistry and Molecular Biology and Infection and Immunity 
      Program, Biomedicine Discovery Institute , Monash University , Clayton , VIC , 
      Australia.
FAU - Croft, Nathan P
AU  - Croft NP
AD  - a Department of Biochemistry and Molecular Biology and Infection and Immunity 
      Program, Biomedicine Discovery Institute , Monash University , Clayton , VIC , 
      Australia.
FAU - Illing, Patricia T
AU  - Illing PT
AD  - a Department of Biochemistry and Molecular Biology and Infection and Immunity 
      Program, Biomedicine Discovery Institute , Monash University , Clayton , VIC , 
      Australia.
FAU - Faridi, Pouya
AU  - Faridi P
AD  - a Department of Biochemistry and Molecular Biology and Infection and Immunity 
      Program, Biomedicine Discovery Institute , Monash University , Clayton , VIC , 
      Australia.
FAU - Purcell, Anthony W
AU  - Purcell AW
AUID- ORCID: 0000-0003-0532-8331
AD  - a Department of Biochemistry and Molecular Biology and Infection and Immunity 
      Program, Biomedicine Discovery Institute , Monash University , Clayton , VIC , 
      Australia.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20180813
PL  - England
TA  - Expert Rev Proteomics
JT  - Expert review of proteomics
JID - 101223548
RN  - 0 (HLA Antigens)
RN  - 0 (Peptides)
SB  - IM
MH  - Alternative Splicing
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Peptides/chemistry
MH  - Protein Processing, Post-Translational
MH  - Proteomics/*methods
OTO - NOTNLM
OT  - PTM
OT  - antigen presentation
OT  - immunopeptidomics
EDAT- 2018/08/07 06:00
MHDA- 2019/04/30 06:00
CRDT- 2018/08/07 06:00
PHST- 2018/08/07 06:00 [pubmed]
PHST- 2019/04/30 06:00 [medline]
PHST- 2018/08/07 06:00 [entrez]
AID - 10.1080/14789450.2018.1509000 [doi]
PST - ppublish
SO  - Expert Rev Proteomics. 2018 Aug;15(8):637-645. doi: 
      10.1080/14789450.2018.1509000. Epub 2018 Aug 13.