PMID- 30081007
OWN - NLM
STAT- MEDLINE
DCOM- 20181003
LR  - 20190816
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 209
DP  - 2018 Sep 15
TI  - Tumor necrosis factor-alpha suppresses the invasion of HTR-8/SVneo trophoblast 
      cells through microRNA-145-5p-mediated downregulation of Cyr61.
PG  - 132-139
LID - S0024-3205(18)30451-X [pii]
LID - 10.1016/j.lfs.2018.08.005 [doi]
AB  - Deficiency in trophoblast invasion is causally linked to the pathogenesis of 
      preeclampsia. Tumor necrosis factor-alpha (TNF-alpha) shows the ability to suppress 
      the invasiveness of trophoblasts, while cysteine-rich 61 (Cyr61) exerts an 
      opposite function in trophoblast invasion. This study was designed to check the 
      hypothesis that cysteine-rich 61 (Cyr61) may be involved in the anti-invasive 
      activity of TNF-alpha in trophoblasts. To this end, we examined the effect of TNF-alpha 
      treatment on Cyr61 expression in HTR-8/SVneo trophoblast cells and investigated 
      the mechanism for the regulation of Cyr61 by TNF-alpha. Gain-of-function experiments 
      were performed to clarify the role of Cyr61 in TNF-alpha-dependent suppression of 
      trophoblast invasion. It was found that TNF-alpha at 1 and 10 ng/mL reduced Cyr61 
      protein levels by 30 and 80%, respectively, in HTR-8/SVneo cells, but did not 
      affect the mRNA expression of Cyr61. Mechanistically, microRNA (miR)-145-5p was 
      stimulated by TNF-alpha and negatively regulated the expression of Cyr61 via 
      interaction with its 3'-untranslated region. Functionally, overexpression of 
      miR-145-5p significantly impaired the migration and invasion of HTR-8/SVneo 
      cells. Depletion of miR-145-5p rescued HTR-8/SVneo cells from TNF-alpha-mediated 
      invasion suppression, which coincided with prevention of Cyr61 downregulation by 
      TNF-alpha. In addition, overexpression of Cyr61 partially restored the invasion of 
      HTR8/SVneo cells co-transfected with miR-145-5p mimic or exposed to TNF-alpha. Taken 
      together, miR-145-5p-mediated downregulation of Cyr61 is required for the 
      anti-invasive effect of TNF-alpha on trophoblasts.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Wen, Zhengfang
AU  - Wen Z
AD  - Reproductive Medical Center, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning, China; Departments of Gynecology and Obstetrics, The First 
      Affiliated Hospital of Xinxiang Medical University, Weihui, China.
FAU - Chen, Yue
AU  - Chen Y
AD  - Department of Obstetrics, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
FAU - Long, Yu
AU  - Long Y
AD  - Department of Obstetrics, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
FAU - Yu, Jian
AU  - Yu J
AD  - Department of Pathology, The First Affiliated Hospital of Xinxiang Medical 
      University, Nanning, China.
FAU - Li, Mujun
AU  - Li M
AD  - Reproductive Medical Center, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning, China. Electronic address: wzf120@126.com.
LA  - eng
PT  - Journal Article
DEP - 20180803
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (CCN1 protein, human)
RN  - 0 (Cysteine-Rich Protein 61)
RN  - 0 (MIRN145 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cells, Cultured
MH  - Cysteine-Rich Protein 61/genetics/*metabolism
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Trophoblasts/*drug effects/metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
OTO - NOTNLM
OT  - Cyr61
OT  - Invasion
OT  - Preeclampsia
OT  - TNF-alpha
OT  - microRNA
EDAT- 2018/08/07 06:00
MHDA- 2018/10/04 06:00
CRDT- 2018/08/07 06:00
PHST- 2018/04/26 00:00 [received]
PHST- 2018/07/26 00:00 [revised]
PHST- 2018/08/02 00:00 [accepted]
PHST- 2018/08/07 06:00 [pubmed]
PHST- 2018/10/04 06:00 [medline]
PHST- 2018/08/07 06:00 [entrez]
AID - S0024-3205(18)30451-X [pii]
AID - 10.1016/j.lfs.2018.08.005 [doi]
PST - ppublish
SO  - Life Sci. 2018 Sep 15;209:132-139. doi: 10.1016/j.lfs.2018.08.005. Epub 2018 Aug 
      3.