PMID- 30081043
OWN - NLM
STAT- MEDLINE
DCOM- 20190715
LR  - 20190715
IS  - 1873-5169 (Electronic)
IS  - 0196-9781 (Linking)
VI  - 107
DP  - 2018 Sep
TI  - RFamide-related peptide-3 promotes alpha TC1 clone 6 cell survival likely via 
      GPR147.
PG  - 39-44
LID - S0196-9781(18)30141-4 [pii]
LID - 10.1016/j.peptides.2018.07.009 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is closely related to islet alpha cell mass and 
      viability. Several types of RFamide-related peptides (RFRPs) are involved in 
      regulating proliferation and function of islet cells. However, current 
      understanding of the role of RFamide-related peptide-3 (RFRP-3) in pancreatic 
      alpha cells is limited. Therefore, we investigated the expression of the RFRP-3 
      receptor, G protein-coupled receptor 147 (GPR147), in mouse islets and alpha TC1 
      clone 6 cells, and evaluated the function of RFRP-3 on alpha cells. We show that 
      GPR147 is expressed in mouse islets and alpha cell lines. In addition, RFRP-3 
      promotes survival of alpha cells under conditions of hyperglycemia and serum 
      starvation. Mechanistic evidence demonstrates that RFRP-3 activated PI3K/AKT and 
      ERK1/2 signaling cascades and treatment with an antagonist of GPR147, 
      1-adamantanecarbonyl-Arg-Phe-NH(2) (RF9) blocked this function. These findings 
      indicate a novel effect of RFRP-3 in promoting alpha cell survival, likely via 
      GPR147.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Zhang, Wencheng
AU  - Zhang W
AD  - Department of Endocrinology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an, 169 Changle Road West, 710032, China.
FAU - Wang, Li
AU  - Wang L
AD  - Department of Endocrinology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an, 169 Changle Road West, 710032, China.
FAU - Yu, Xinwen
AU  - Yu X
AD  - Department of Endocrinology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an, 169 Changle Road West, 710032, China.
FAU - Jia, Aihua
AU  - Jia A
AD  - Department of Endocrinology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an, 169 Changle Road West, 710032, China.
FAU - Ming, Jie
AU  - Ming J
AD  - Department of Endocrinology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an, 169 Changle Road West, 710032, China.
FAU - Ji, Qiuhe
AU  - Ji Q
AD  - Department of Endocrinology, Xijing Hospital, Fourth Military Medical University, 
      Xi'an, 169 Changle Road West, 710032, China. Electronic address: 
      qiuheji@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180803
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Neuropeptides)
RN  - 0 (RFamide peptide)
RN  - 0 (Receptors, Neuropeptide)
RN  - 0 (neuropeptide FF receptor)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Glucagon-Secreting Cells/*metabolism/physiology
MH  - MAP Kinase Signaling System
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neuropeptides/*metabolism/physiology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Receptors, Neuropeptide/*metabolism/physiology
MH  - *Signal Transduction
OTO - NOTNLM
OT  - 1-adamantanecarbonyl-Arg-Phe-NH(2)(RF9)
OT  - G protein-coupled receptor 147 (GPR147)
OT  - Gonadotropin inhibitory hormone (GnIH)
OT  - RFamide-related peptide-3 (RFRP-3)
EDAT- 2018/08/07 06:00
MHDA- 2019/07/16 06:00
CRDT- 2018/08/07 06:00
PHST- 2018/04/12 00:00 [received]
PHST- 2018/07/08 00:00 [revised]
PHST- 2018/07/31 00:00 [accepted]
PHST- 2018/08/07 06:00 [pubmed]
PHST- 2019/07/16 06:00 [medline]
PHST- 2018/08/07 06:00 [entrez]
AID - S0196-9781(18)30141-4 [pii]
AID - 10.1016/j.peptides.2018.07.009 [doi]
PST - ppublish
SO  - Peptides. 2018 Sep;107:39-44. doi: 10.1016/j.peptides.2018.07.009. Epub 2018 Aug 
      3.