PMID- 30082606 OWN - NLM STAT- MEDLINE DCOM- 20190523 LR - 20190523 IS - 2072-6651 (Electronic) IS - 2072-6651 (Linking) VI - 10 IP - 8 DP - 2018 Aug 6 TI - Development of a Magnetic Nanoparticles-Based Screen-Printed Electrodes (MNPs-SPEs) Biosensor for the Quantification of Ochratoxin A in Cereal and Feed Samples. LID - 10.3390/toxins10080317 [doi] LID - 317 AB - A rapid and sensitive electrochemical biosensor based on magnetic nanoparticles and screen-printed electrodes (MNPs-SPEs sensor) was developed for the detection of ochratoxin A (OTA) in cereal and feed samples. Different types of magnetic nanoparticles-based ELISA (MNPs-ELISA) were optimized, and the signal detection, as well as sensitivity, was enhanced by the combined use of screen-printed electrodes (SPEs). Under the optimized conditions, the calibration curve of the MNPs-SPEs sensor was y = 0.3372x + 0.8324 (R(2) = 0.9805). The linear range of detection and the detection limit were 0.01(-)0.82 ng/mL and 0.007 ng/mL, respectively. In addition, 50% inhibition (IC50) was detectable at 0.10 ng/mL. The limit of detection (LOD) of this MNPs-SPEs sensor in cereal and feed samples was 0.28 mug/kg. The recovery rates in spiked samples were between 78.7% and 113.5%, and the relative standard deviations (RSDs) were 3.6(-)9.8%, with the coefficient of variation lower than 15%. Parallel analysis of commercial samples (corn, wheat, and feedstuff) showed a good correlation between MNPs-SPEs sensor and liquid chromatography tandem mass spectrometry (LC/MS-MS). This new method provides a rapid, highly sensitive, and less time-consuming method to determine levels of ochratoxin A in cereal and feedstuff samples. FAU - Zhang, Xian AU - Zhang X AD - China-Australian Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, Zhejiang A&F University, Lin'an 311300, China. zhangxian073@163.com. AD - Zhejiang University Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, 388 Yuhangtang Road, Hangzhou 310058, China. zhangxian073@163.com. FAU - Wang, Zuohuan AU - Wang Z AD - Zhejiang University Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, 388 Yuhangtang Road, Hangzhou 310058, China. zuohuanwang@zju.edu.cn. FAU - Xie, Hui AU - Xie H AD - Zhejiang Key Laboratory of Experimental Animal and Safety Evaluation, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China. xhui828@163.com. FAU - Sun, Renjie AU - Sun R AD - Zhejiang University Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, 388 Yuhangtang Road, Hangzhou 310058, China. rjsun@zju.edu.cn. FAU - Cao, Tong AU - Cao T AD - Zhejiang University Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, 388 Yuhangtang Road, Hangzhou 310058, China. caotong0915@zju.edu.cn. FAU - Paudyal, Narayan AU - Paudyal N AUID- ORCID: 0000-0001-8204-2674 AD - Zhejiang University Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, 388 Yuhangtang Road, Hangzhou 310058, China. narayan.paudyal@outlook.com. FAU - Fang, Weihuan AU - Fang W AD - China-Australian Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, Zhejiang A&F University, Lin'an 311300, China. whfang@zju.edu.cn. AD - Zhejiang University Institute of Preventive Veterinary Medicine & Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, 388 Yuhangtang Road, Hangzhou 310058, China. whfang@zju.edu.cn. FAU - Song, Houhui AU - Song H AD - China-Australian Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, College of Animal Science and Technology, Zhejiang A&F University, Lin'an 311300, China. songhh@zafu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180806 PL - Switzerland TA - Toxins (Basel) JT - Toxins JID - 101530765 RN - 0 (Antibodies) RN - 0 (Ochratoxins) RN - 1779SX6LUY (ochratoxin A) RN - 27432CM55Q (Serum Albumin, Bovine) RN - EC 1.11.1.- (Horseradish Peroxidase) SB - IM MH - Animal Feed/*analysis MH - Antibodies/immunology MH - *Biosensing Techniques MH - Edible Grain/*chemistry MH - Electrodes MH - Enzyme-Linked Immunosorbent Assay MH - Food Contamination/*analysis MH - Horseradish Peroxidase/immunology MH - Magnetic Phenomena MH - Nanoparticles/*chemistry MH - Ochratoxins/*analysis/immunology MH - Serum Albumin, Bovine/immunology MH - Triticum MH - Zea mays PMC - PMC6115714 OTO - NOTNLM OT - magnetic nanoparticles OT - ochratoxin A OT - quantification OT - screen-printed electrodes COIS- The authors declare no conflict of interest. EDAT- 2018/08/08 06:00 MHDA- 2019/05/24 06:00 PMCR- 2018/08/01 CRDT- 2018/08/08 06:00 PHST- 2018/06/12 00:00 [received] PHST- 2018/08/02 00:00 [revised] PHST- 2018/08/03 00:00 [accepted] PHST- 2018/08/08 06:00 [entrez] PHST- 2018/08/08 06:00 [pubmed] PHST- 2019/05/24 06:00 [medline] PHST- 2018/08/01 00:00 [pmc-release] AID - toxins10080317 [pii] AID - toxins-10-00317 [pii] AID - 10.3390/toxins10080317 [doi] PST - epublish SO - Toxins (Basel). 2018 Aug 6;10(8):317. doi: 10.3390/toxins10080317.