PMID- 3008574
OWN - NLM
STAT- MEDLINE
DCOM- 19860515
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 250
IP  - 4 Pt 1
DP  - 1986 Apr
TI  - Intestinal anaphylaxis in the rat: jejunal response to in vitro antigen exposure.
PG  - G427-31
AB  - In previous studies we showed that rats sensitized to egg albumin respond to in 
      vivo intraluminal antigen with decreased net absorption of Na+, Cl-, and water. 
      These abnormalities are associated with high serum levels of immunoglobulin E 
      (IgE) antibodies and mucosal mast cell degranulation. In the present in vitro 
      study electrical parameters, unidirectional fluxes of Na+ and Cl-, and levels of 
      cAMP were determined in jejunum from sensitized and control rats during a basal 
      period and after antigen addition. In Ussing chambers potential difference and 
      short-circuit current increased significantly in tissue from sensitized rats 
      after addition of 100 micrograms/ml of egg albumin to both mucosal and serosal 
      surfaces. These changes were accompanied by a reversal of net Cl- absorption to 
      net Cl- secretion. The presence of doxantrazole, a mast cell-stabilizing agent, 
      in the buffer prevented these abnormalities. No changes occurred in response to 
      antigen challenge in tissue from controls. In a further series of experiments the 
      antigen was added only to the mucosal side of the tissue in Ussing chambers. In 
      these studies short-circuit current increased after a lag period of approximately 
      25 min and was significantly increased (P less than 0.025) at 35 min. cAMP levels 
      increased significantly in jejunal slices from sensitized rats exposed to antigen 
      for 2 min. Our findings suggest that the in vivo transport abnormalities induced 
      by IgE-mediated mucosal reactions to a food protein are related to antigen 
      stimulation of a Cl- secretory process.
FAU - Perdue, M H
AU  - Perdue MH
FAU - Gall, D G
AU  - Gall DG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Antigens)
RN  - 0 (Chlorides)
RN  - 0 (Thioxanthenes)
RN  - 0 (Xanthones)
RN  - 9NEZ333N27 (Sodium)
RN  - E0399OZS9N (Cyclic AMP)
RN  - U5Q9UW0HCH (doxantrazole)
SB  - IM
MH  - Anaphylaxis/*physiopathology
MH  - Animals
MH  - Antigens/*pharmacology
MH  - Body Water/metabolism
MH  - Chlorides/metabolism
MH  - Cyclic AMP/metabolism
MH  - *Intestinal Absorption/drug effects
MH  - Jejunum/drug effects/*physiopathology
MH  - Mucous Membrane/metabolism
MH  - Rats
MH  - Serous Membrane/metabolism
MH  - Sodium/metabolism
MH  - Thioxanthenes/pharmacology
MH  - Xanthones
EDAT- 1986/04/01 00:00
MHDA- 1986/04/01 00:01
CRDT- 1986/04/01 00:00
PHST- 1986/04/01 00:00 [pubmed]
PHST- 1986/04/01 00:01 [medline]
PHST- 1986/04/01 00:00 [entrez]
AID - 10.1152/ajpgi.1986.250.4.G427 [doi]
PST - ppublish
SO  - Am J Physiol. 1986 Apr;250(4 Pt 1):G427-31. doi: 10.1152/ajpgi.1986.250.4.G427.