PMID- 30086883
OWN - NLM
STAT- MEDLINE
DCOM- 20190102
LR  - 20190102
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 503
IP  - 4
DP  - 2018 Sep 18
TI  - Transcriptome of porcine alveolar macrophages activated by interferon-gamma and 
      lipopolysaccharide.
PG  - 2666-2672
LID - S0006-291X(18)31695-4 [pii]
LID - 10.1016/j.bbrc.2018.08.021 [doi]
AB  - The molecular repertoire of porcine alveolar macrophages (PAMs) is greatly 
      affected by the microenvironment they are exposed to, and specifically by 
      inflammatory cytokines, such as interferon gamma (IFN-gamma) released by activated 
      lymphocytes, and microbial products, such as lipopolysaccharide (LPS). In our 
      previous study, we found that IFN-gamma- and LPS-activated PAMs (M1) could inhibit 
      porcine reproductive and respiratory syndrome virus (PRRSV) replication. In this 
      study, comprehensive analysis of the expression profiles of the genes associated 
      with the polarization of M0-type PAMs (resting) toward M1 phenotypes (activated 
      by IFN-gamma and LPS) led to the following main results: 1) 1551 and 1823 genes were 
      upregulated or downregulated in M1-type PAMs, respectively, compared with M0-type 
      PAMs; 2) Among these, genes encoding ASS1 and CRTAM were the most upregulated and 
      downregulated, respectively; 3) Genes involved in cytokine-cytokine receptor 
      interaction and the JAK/STAT signaling pathway were significantly upregulated, 
      suggesting their critical role in cellular activation; and 4) Genes involved in 
      antigen proteolysis and presentation (immunoproteasome subunits), and inhibition 
      of virus replication (host restriction factors) were significantly upregulated, 
      emphasizing the critical role of these cytokines in immunity. Thus, our results 
      provide important information for future studies on the role of PAM polarization 
      in modulation of infection.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Liu, Qiang
AU  - Liu Q
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: liuqiang.@caas.cn.
FAU - Zhang, Yong-Li
AU  - Zhang YL
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: 13949054838@126.com.
FAU - Hu, Wei
AU  - Hu W
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: huwei05190806y@163.com.
FAU - Hu, Shou-Ping
AU  - Hu SP
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: hshp02@126.com.
FAU - Zhang, Zhuo
AU  - Zhang Z
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: clearzhang1006@sina.com.
FAU - Cai, Xue-Hui
AU  - Cai XH
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: aci139@sina.com.
FAU - He, Xi-Jun
AU  - He XJ
AD  - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute of Chinese Academy of Agricultural Sciences, Harbin, China. Electronic 
      address: hexijun@caas.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180804
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Immunoglobulins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Cytokine)
RN  - 0 (STAT Transcription Factors)
RN  - 0 (class-I restricted T cell-associated molecule)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.10.2 (Janus Kinase 1)
RN  - EC 6.3.4.5 (Argininosuccinate Synthase)
SB  - IM
MH  - Animals
MH  - Argininosuccinate Synthase/*genetics/immunology
MH  - Cell Differentiation/drug effects
MH  - Cell Lineage/drug effects
MH  - Gene Ontology
MH  - Immunoglobulins/*genetics/immunology
MH  - Interferon-gamma/*pharmacology
MH  - Janus Kinase 1/genetics/immunology
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages, Alveolar/*drug effects/immunology/virology
MH  - Molecular Sequence Annotation
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/immunology
MH  - Primary Cell Culture
MH  - Receptors, Cytokine/genetics/immunology
MH  - STAT Transcription Factors/genetics/immunology
MH  - Swine
MH  - Transcriptome/*immunology
MH  - Virus Replication/drug effects
OTO - NOTNLM
OT  - Host restriction factor
OT  - Immunoproteasome
OT  - Interferon gamma
OT  - Lipopolysaccharide
OT  - Porcine alveolar macrophages
OT  - RNA sequencing
EDAT- 2018/08/09 06:00
MHDA- 2019/01/03 06:00
CRDT- 2018/08/09 06:00
PHST- 2018/08/01 00:00 [received]
PHST- 2018/08/02 00:00 [accepted]
PHST- 2018/08/09 06:00 [pubmed]
PHST- 2019/01/03 06:00 [medline]
PHST- 2018/08/09 06:00 [entrez]
AID - S0006-291X(18)31695-4 [pii]
AID - 10.1016/j.bbrc.2018.08.021 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Sep 18;503(4):2666-2672. doi: 
      10.1016/j.bbrc.2018.08.021. Epub 2018 Aug 4.