PMID- 30090485
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240329
IS  - 2045-452X (Print)
IS  - 2045-4538 (Electronic)
IS  - 2045-452X (Linking)
VI  - 6
IP  - 2
DP  - 2017 Mar 1
TI  - Evaluation of the DNA damaging potential of indigenous health hazardous quartz 
      nanoparticles on the cultured lung cells.
PG  - 152-161
LID - 10.1039/c6tx00414h [doi]
AB  - Quartz nanoparticles (QNPs) cause various diseases in the biological systems and 
      are thus considered as hazardous materials; however, their properties are 
      important in a wide range of biological and pharmaceutical entities. The present 
      study was initiated to enhance the understanding of the genotoxic potential and 
      focused on the mechanism involved in the indigenous quartz nanoparticles induced 
      toxicity in A549 cells. The results show a concentration dependent decrease in 
      the cell viability of A549 cells. Additionally, QNPs exposure results in the 
      concentration dependent toxicity, which intensifies the generation of ROS, which 
      in turn is confirmed by the increased lipid peroxidation and decreased GSH 
      levels. Moreover, it also induces DNA damage in the cells exposed to QNPs as 
      compared to the control cells. The outcome of the results confirms that QNPs have 
      cytotoxic and genotoxic effects on the A549 cells in a concentration and time 
      dependent manner. Western blot analysis indicates the up regulation of LC-3I/II, 
      Beclin-1, and p62 protein levels, which confirms the occurrence of autophagy 
      through the phosphatidylinositol 3-kinase (PI3K)/a threonine kinase 
      (Akt)/mammalian target of rapamycin (mTOR) pathway, as well as the inhibitor of 
      autophagy is also verified. Our study demonstrates that QNPs exposure in human 
      lung cells causes oxidative stress and DNA damage, which leads to autophagy. 
      Hence, from a bioremediation point of view, the remedial strategy that can be 
      adopted from the biological DNA damage from these health hazardous materials will 
      result in a safer environment.
FAU - Sushma
AU  - Sushma
AD  - Fiber Toxicology Division , CSIR-Indian Institute of Toxicology Research , PO 
      Box-80 , MG Marg , Lucknow-226001 , India.
FAU - Kumar, Hridyesh
AU  - Kumar H
AD  - Department of Chemistry , Motilal Nehru National Institute of Technology , 
      Allahabad 211004 , India . Email: pkd_437@yahoo.com.
FAU - Ahmad, Iqbal
AU  - Ahmad I
AD  - Fiber Toxicology Division , CSIR-Indian Institute of Toxicology Research , PO 
      Box-80 , MG Marg , Lucknow-226001 , India.
FAU - Dutta, Pradip Kumar
AU  - Dutta PK
AUID- ORCID: 0000-0002-3185-3888
AD  - Department of Chemistry , Motilal Nehru National Institute of Technology , 
      Allahabad 211004 , India . Email: pkd_437@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20161220
PL  - England
TA  - Toxicol Res (Camb)
JT  - Toxicology research
JID - 101587950
PMC - PMC6061934
EDAT- 2016/12/20 00:00
MHDA- 2016/12/20 00:01
PMCR- 2017/12/20
CRDT- 2018/08/10 06:00
PHST- 2016/11/07 00:00 [received]
PHST- 2016/12/19 00:00 [accepted]
PHST- 2018/08/10 06:00 [entrez]
PHST- 2016/12/20 00:00 [pubmed]
PHST- 2016/12/20 00:01 [medline]
PHST- 2017/12/20 00:00 [pmc-release]
AID - c6tx00414h [pii]
AID - 10.1039/c6tx00414h [doi]
PST - epublish
SO  - Toxicol Res (Camb). 2016 Dec 20;6(2):152-161. doi: 10.1039/c6tx00414h. 
      eCollection 2017 Mar 1.