PMID- 3009094
OWN - NLM
STAT- MEDLINE
DCOM- 19860602
LR  - 20190828
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 5
IP  - 3
DP  - 1986 Mar
TI  - Quantitation and kinetics of induced HSV-1 ocular shedding.
PG  - 241-6
AB  - Iontophoresis of 6-hydroxydopamine (6-HD) to the rabbit eye, followed by topical 
      instillation of 2% epinephrine, induces ocular shedding of herpes simplex virus 
      type 1 (HSV-1) reliably and with a high frequency in latently infected rabbits. 
      Rabbit eyes inoculated with HSV-1 (McKrae strain) showed dendritic lesions 
      indicative of acute HSV infection and subsequently shed virus spontaneously at 
      least once during days 20 to 39 postinoculation (P.I.). Two iontophoretic 
      conditions were employed. Group A (3 rabbits, 60.3 days P.I.) received 
      iontophoresis of 1.0% 6-HD at 0.75 mAmp for 3 min. Group B (three rabbits, 67.3 
      days P.I.) received iontophoresis of 0.1% 6-HD at 0.5 mAmp for 8 min. Following 
      iontophoresis, 2% epinephrine was instilled topically once on the day of 
      iontophoresis and twice daily for four consecutive days. Tear film was collected 
      on Dacron swabs and titered on African green monkey kidney cells by a plaque 
      assay procedure. In group A, 100% (6/6) of the eyes shed virus, and the average 
      duration of shedding was 4.0 days. The titers ranged from 2.0 to 7.7 X 10(4) 
      plaque-forming units (PFU) per eye. The highest daily average titer, 9.89 X 10(3) 
      PFU/eye, occurred on day 5 following iontophoresis. In Group B, 100% (6/6) of the 
      eyes also shed virus, and the average duration of shedding was 5.3 days. The 
      viral titer of the tear film ranged from 5.0 to 1.4 X 10(5) PFU/eye. The highest 
      daily average titer, 4.68 X 10(3) PFU/eye, also occurred on day 5 following 
      iontophoresis.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Hill, J M
AU  - Hill JM
FAU - Dudley, J B
AU  - Dudley JB
FAU - Shimomura, Y
AU  - Shimomura Y
FAU - Kaufman, H E
AU  - Kaufman HE
LA  - eng
GR  - EY02389/EY/NEI NIH HHS/United States
GR  - EY02672/EY/NEI NIH HHS/United States
GR  - EY06311/EY/NEI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Hydroxydopamines)
RN  - 8HW4YBZ748 (Oxidopamine)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Animals
MH  - Epinephrine/administration & dosage
MH  - Hydroxydopamines/administration & dosage
MH  - Iontophoresis
MH  - Keratitis, Dendritic/*microbiology
MH  - Kinetics
MH  - Oxidopamine
MH  - Rabbits
MH  - Simplexvirus/*isolation & purification
MH  - Tears/*microbiology
MH  - Viral Plaque Assay
EDAT- 1986/03/01 00:00
MHDA- 1986/03/01 00:01
CRDT- 1986/03/01 00:00
PHST- 1986/03/01 00:00 [pubmed]
PHST- 1986/03/01 00:01 [medline]
PHST- 1986/03/01 00:00 [entrez]
AID - 10.3109/02713688609020049 [doi]
PST - ppublish
SO  - Curr Eye Res. 1986 Mar;5(3):241-6. doi: 10.3109/02713688609020049.