PMID- 30091231
OWN - NLM
STAT- MEDLINE
DCOM- 20190107
LR  - 20190107
IS  - 1432-2277 (Electronic)
IS  - 0934-0874 (Linking)
VI  - 31
IP  - 11
DP  - 2018 Nov
TI  - Technical challenges and clinical relevance of single antigen bead C1q/C3d 
      testing and IgG subclass analysis of human leukocyte antigen antibodies.
PG  - 1189-1197
LID - 10.1111/tri.13327 [doi]
AB  - Luminex single antigen bead assays revolutionized human leukocyte antigen (HLA) 
      antibody detection owing to their superior sensitivity compared to conventional 
      methods. Nevertheless, the advent of higher sensitivity came at the expense of 
      difficulty in clinical decision-making, since not all luminex detectable 
      antibodies are clinically relevant. Therefore, new tools such as C1q/C3d assays 
      and IgG subclass analysis emerged with the aim to discriminate the inert 
      antibodies from the deleterious ones. Here, we provide an overview on the 
      technical challenges related to these different HLA antibody detection systems 
      and briefly refer to the recent literature regarding the clinical relevance of 
      these assays, mainly in the field of kidney transplantation.
CI  - (c) 2018 Steunstichting ESOT.
FAU - Karahan, Gonca E
AU  - Karahan GE
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, the Netherlands.
FAU - Claas, Frans H J
AU  - Claas FHJ
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, the Netherlands.
FAU - Heidt, Sebastiaan
AU  - Heidt S
AUID- ORCID: 0000-0002-6700-188X
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, the Netherlands.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20180824
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
RN  - 0 (HLA Antigens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Isoantibodies)
RN  - 80295-33-6 (Complement C1q)
RN  - 80295-45-0 (Complement C3d)
SB  - IM
MH  - Complement C1q/*chemistry
MH  - Complement C3d/*chemistry
MH  - Decision Support Systems, Clinical
MH  - Graft Rejection/immunology
MH  - HLA Antigens/*chemistry
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Immunoglobulin G/*chemistry
MH  - Isoantibodies/immunology
MH  - Kidney Transplantation
MH  - Postoperative Period
MH  - Prognosis
MH  - Protein Binding
MH  - Risk
OTO - NOTNLM
OT  - antibody-mediated rejection
OT  - donor-specific antibody
OT  - kidney transplantation
OT  - luminex
EDAT- 2018/08/10 06:00
MHDA- 2019/01/08 06:00
CRDT- 2018/08/10 06:00
PHST- 2018/05/31 00:00 [received]
PHST- 2018/06/21 00:00 [revised]
PHST- 2018/08/03 00:00 [accepted]
PHST- 2018/08/10 06:00 [pubmed]
PHST- 2019/01/08 06:00 [medline]
PHST- 2018/08/10 06:00 [entrez]
AID - 10.1111/tri.13327 [doi]
PST - ppublish
SO  - Transpl Int. 2018 Nov;31(11):1189-1197. doi: 10.1111/tri.13327. Epub 2018 Aug 24.