PMID- 30092387 OWN - NLM STAT- MEDLINE DCOM- 20181126 LR - 20181126 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 164 DP - 2018 Nov 30 TI - The influence of Fe(2+), Fe(3+) and magnet powder (Fe(3)O(4)) on aerobic granulation and their mechanisms. PG - 1-11 LID - S0147-6513(18)30669-9 [pii] LID - 10.1016/j.ecoenv.2018.07.072 [doi] AB - This study aimed to develop an aerobic granular sludge and understand the granulation process of the multi-iron ions. Four sequencing batch reactors (SBRs) were applied to elucidate the effect of Fe(2+), Fe(3+) and Fe(3)O(4) addition on aerobic granulation. The results confirmed that the start-up time of aerobic granulation with Fe(3)O(4) addition (11 days) was notably less than that with Fe(2+) (16 days) and Fe(3+) (27 days) addition. Larger granules achieved with Fe(3)O(4) addition with a sludge volume index (SVI(30)) of 28.50 mL/g and settling velocity of 49.68 m/h. Scanning electron microscope (SEM) analysis further revealed that the presence of mineral crystal in the granule core with Fe(2+) and Fe(3)O(4) addition accelerated the granule formation and maintained the stability of the structure. Extracellular polymeric substances (EPS) were studied using three-dimensional-excitation emission matrix (3D-EEM) fluorescence spectra technology to gain a comprehensive view of the interactions between EPS and Fe(2+), Fe(3+) and Fe(3)O(4). Around 94.76% and 97.68% removal rate was noted for COD and ammonia in the granulation process. Finally, the dominant functional species involved in biological nutrients removal and granule formation were identified by high throughput sequencing technology to assess the effects of Fe(2+), Fe(3+) and Fe(3)O(4) to granule at the molecular level. CI - Copyright (c) 2018 Elsevier Inc. All rights reserved. FAU - Ren, Xiaomin AU - Ren X AD - College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. FAU - Chen, Yue AU - Chen Y AD - College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. FAU - Guo, Liang AU - Guo L AD - College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Key Laboratory of Marine Environmental and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China. Electronic address: geletu@ouc.edu.cn. FAU - She, Zonglian AU - She Z AD - College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. FAU - Gao, Mengchun AU - Gao M AD - College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. FAU - Zhao, Yangguo AU - Zhao Y AD - College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. FAU - Shao, Mengyu AU - Shao M AD - College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. LA - eng PT - Journal Article DEP - 20180806 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 RN - 0 (Biopolymers) RN - 0 (Powders) RN - 0 (Sewage) RN - 7664-41-7 (Ammonia) RN - E1UOL152H7 (Iron) RN - XM0M87F357 (Ferrosoferric Oxide) SB - IM MH - Aerobiosis MH - Ammonia MH - Biopolymers MH - *Bioreactors MH - *Ferrosoferric Oxide MH - *Iron MH - Powders MH - Sewage MH - Waste Disposal, Fluid OTO - NOTNLM OT - Aerobic granular sludge OT - Aerobic granulation OT - Fe(2+) OT - Fe(3+) OT - Magnet powder (Fe(3)O(4)) EDAT- 2018/08/10 06:00 MHDA- 2018/11/27 06:00 CRDT- 2018/08/10 06:00 PHST- 2018/02/03 00:00 [received] PHST- 2018/07/18 00:00 [revised] PHST- 2018/07/19 00:00 [accepted] PHST- 2018/08/10 06:00 [pubmed] PHST- 2018/11/27 06:00 [medline] PHST- 2018/08/10 06:00 [entrez] AID - S0147-6513(18)30669-9 [pii] AID - 10.1016/j.ecoenv.2018.07.072 [doi] PST - ppublish SO - Ecotoxicol Environ Saf. 2018 Nov 30;164:1-11. doi: 10.1016/j.ecoenv.2018.07.072. Epub 2018 Aug 6.