PMID- 30092818
OWN - NLM
STAT- MEDLINE
DCOM- 20190219
LR  - 20190320
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 9
IP  - 1
DP  - 2018 Aug 9
TI  - The role of the Wnt/beta-catenin signaling pathway in the proliferation of gold 
      nanoparticle-treated human periodontal ligament stem cells.
PG  - 214
LID - 10.1186/s13287-018-0954-6 [doi]
LID - 214
AB  - BACKGROUND: Several studies have confirmed that gold nanoparticles (AuNPs) of 
      specific concentration and size exert a boosting effect on cell proliferation; 
      however, the mechanism through which this effect occurs remains unknown. This 
      study explores the canonical Wnt signaling pathway in AuNP promotion of human 
      periodontal ligament stem cell (hPDLSC) proliferation. METHODS: MTS was employed 
      to evaluate hPDLSC proliferation. The interference of LRP5 and beta-catenin was 
      steered by shRNA plasmids and siRNA, respectively, at which point the expression 
      of MYC, CCND1, AXIN2, and POU5F1 had been estimated via real-time PCR. The 
      expressions of LRP5 and beta-catenin were detected via western blot assay. RESULTS: 
      The proliferation of hPDLSCs treated with 60 nm AuNPs at 56 muM was clearly 
      elevated. In contrast, beta-catenin siRNA significantly decreased cell viability. 
      The LRP5 shRNA plasmid did not consistently impact cells. The expressions of 
      these four genes downstream of the Wnt/beta-catenin signaling pathway were not 
      significantly overexpressed in response to the interference of shRNA 
      plasmid/siRNA with the treatment of AuNPs. CONCLUSIONS: These results suggest 
      that the Wnt/beta-catenin signaling pathway plays a significant role in the process 
      of AuNP promotion of hPDLSC proliferation.
FAU - Li, Chen
AU  - Li C
AD  - Department of Endodontics, School & Hospital of Stomatology, Tongji University, 
      Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 
      Shanghai, 200072, China.
FAU - Li, Zhuoquan
AU  - Li Z
AD  - The Institute for Translational Nanomedicine, Shanghai East Hospital, The 
      Institute for Biomedical Engineering & Nano Science, Tongji University School of 
      Medicine, Shanghai, 200092, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Department of Basic Science, School & Hospital of Stomatology, Tongji University, 
      Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 
      Shanghai, 200072, China.
FAU - Fathy, Abdel Hamid
AU  - Fathy AH
AD  - Tongji University School of Medicine, Shanghai, 200092, China.
FAU - Zhou, Min
AU  - Zhou M
AD  - Department of Periodontics, School & Hospital of Stomatology, Tongji University, 
      Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 
      Shanghai, 200072, China. kqzhoumin@tongji.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180809
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (RNA, Small Interfering)
RN  - 0 (beta Catenin)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Cell Proliferation/physiology
MH  - Gold/*chemistry
MH  - Humans
MH  - Metal Nanoparticles/*chemistry
MH  - Periodontal Ligament/*cytology
MH  - RNA, Small Interfering/metabolism
MH  - Stem Cells/*cytology
MH  - Wnt Signaling Pathway
MH  - beta Catenin/*metabolism
PMC - PMC6085621
OTO - NOTNLM
OT  - Gold nanoparticle
OT  - Human periodontal ligament stem cell proliferation
OT  - Wnt/beta-catenin signaling pathway
OT  - shRNA
OT  - siRNA
COIS- This work received approval from the Tongji University affiliated Stomatological 
      Hospital Ethics Committee and informed consent was obtained from the LAR of the 
      participant and the participant's guardian as premolar samples were obtained from 
      minors in the study. Not applicable. The authors declare that they have no 
      competing interests. Springer Nature remains neutral with regard to 
      jurisdictional claims in published maps and institutional affiliations.
EDAT- 2018/08/11 06:00
MHDA- 2019/03/21 06:00
PMCR- 2018/08/09
CRDT- 2018/08/11 06:00
PHST- 2018/02/22 00:00 [received]
PHST- 2018/07/10 00:00 [accepted]
PHST- 2018/06/26 00:00 [revised]
PHST- 2018/08/11 06:00 [entrez]
PHST- 2018/08/11 06:00 [pubmed]
PHST- 2019/03/21 06:00 [medline]
PHST- 2018/08/09 00:00 [pmc-release]
AID - 10.1186/s13287-018-0954-6 [pii]
AID - 954 [pii]
AID - 10.1186/s13287-018-0954-6 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2018 Aug 9;9(1):214. doi: 10.1186/s13287-018-0954-6.