PMID- 30092831
OWN - NLM
STAT- MEDLINE
DCOM- 20190219
LR  - 20190320
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 9
IP  - 1
DP  - 2018 Aug 9
TI  - The legacy effects of electromagnetic fields on bone marrow mesenchymal stem cell 
      self-renewal and multiple differentiation potential.
PG  - 215
LID - 10.1186/s13287-018-0955-5 [doi]
LID - 215
AB  - BACKGROUND: The effects of electromagnetic fields (EMF) on bone nonunion have 
      been reported for many years. Many studies and randomized controlled trials have 
      demonstrated that EMF exhibited benefits in curing delayed union and nonunion of 
      long bone fractures. Most of them focused on the immediate effects, while the 
      legacy effects of EMF remain poorly investigated. METHODS: In this study, rat 
      bone marrow mesenchymal stem cells (BMSCs) were treated with EMF, and after a 
      period of time the BMSC proliferation and differentiation were detected. 
      Additionally, BMSC sheets with or without EMF treatment were transplanted into 
      the rat tibia fracture nonunion models. The bone formation was evaluated after 2, 
      4, and 6 weeks. RESULTS: Our results showed that the proliferation capacity of 
      BMSCs was heightened after EMF pretreatment. Over a period of time of EMF 
      pretreatment, the capacities of osteogenic and chondrogenic differentiation were 
      enhanced, while adipogenic differentiation was weakened. BMSC sheets pretreated 
      with EMF could better promote the healing of tibia fracture in rats, compared to 
      BMSC sheets alone. Furthermore, significantly higher values of radiographic 
      grading scores were observed in the EMF group. CONCLUSIONS: EMF has lasting 
      effects on the proliferation and differentiation of BMSCs, and together with cell 
      sheet technology can provide a new method for the treatment of fracture nonunion.
FAU - Tu, Chang
AU  - Tu C
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, People's Republic of China.
FAU - Xiao, Yifan
AU  - Xiao Y
AD  - Department of Immunology, School of Basic Medicine, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of 
      China.
FAU - Ma, Yongzhuang
AU  - Ma Y
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, People's Republic of China.
FAU - Wu, Hua
AU  - Wu H
AD  - Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, People's Republic of China. 
      wuhua360@aliyun.com.
FAU - Song, Mingyu
AU  - Song M
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of 
      China. langzhiwu126@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180809
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Bone Marrow Cells/*cytology/*radiation effects
MH  - Cell Differentiation/radiation effects
MH  - Cell Proliferation/radiation effects
MH  - *Electromagnetic Fields
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/*radiation effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
PMC - PMC6085613
OTO - NOTNLM
OT  - Cell sheets
OT  - Differentiation
OT  - Electromagnetic fields
OT  - Legacy effects
OT  - Mesenchymal stem cells
OT  - Proliferation
COIS- All animal experiments in this study were performed in strict accordance with the 
      Institutional Animal Care and Use Committee, Tongji Medical College, Huazhong 
      University of Science and Technology. All efforts were made to minimize animal 
      suffering. All authors have agreed to submit this manuscript for publication. The 
      authors declare that they have no competing interests. Springer Nature remains 
      neutral with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2018/08/11 06:00
MHDA- 2019/03/21 06:00
PMCR- 2018/08/09
CRDT- 2018/08/11 06:00
PHST- 2018/05/14 00:00 [received]
PHST- 2018/07/10 00:00 [accepted]
PHST- 2018/06/28 00:00 [revised]
PHST- 2018/08/11 06:00 [entrez]
PHST- 2018/08/11 06:00 [pubmed]
PHST- 2019/03/21 06:00 [medline]
PHST- 2018/08/09 00:00 [pmc-release]
AID - 10.1186/s13287-018-0955-5 [pii]
AID - 955 [pii]
AID - 10.1186/s13287-018-0955-5 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2018 Aug 9;9(1):215. doi: 10.1186/s13287-018-0955-5.