PMID- 30094297
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230928
IS  - 2352-1872 (Print)
IS  - 2352-1872 (Electronic)
IS  - 2352-1872 (Linking)
VI  - 9
DP  - 2018 Dec
TI  - Characteristics of bone strength and metabolism in type 2 diabetic model Tsumura, 
      Suzuki, Obese Diabetes mice.
PG  - 74-83
LID - 10.1016/j.bonr.2018.07.004 [doi]
AB  - OBJECTIVE: Type 2 diabetes mellitus (T2DM) is a metabolic disease characterized 
      by hyperglycemia, hyperinsulinemia, and complications such as obesity and 
      osteoporosis. The Tsumura, Suzuki, Obese Diabetes (TSOD) mouse is an animal model 
      of spontaneous obese T2DM. However, bone metabolism in TSOD mice is yet to be 
      investigated. The objective of the present study was to investigate the effects 
      of T2DM on bone mass, metabolism, microstructure, and strength in TSOD mice. 
      METHODS: We determined the following parameters in TSOD mice and Tsumura, Suzuki, 
      Non-obesity (TSNO) mice (as controls): serum glucose levels; serum insulin 
      levels; bone mass; bone microstructure; bone metabolic markers; and bone 
      strength. We also performed the oral glucose tolerance test and examined 
      histological sections of the femur. We compared these data between both groups at 
      pre-diabetic (10 weeks) and established (20 weeks) diabetic conditions. RESULTS: 
      Bone strength, such as extrinsic mechanical properties, increased with age in the 
      TSOD mice and intrinsic material properties decreased at both 10 weeks and 
      20 weeks. Bone resorption marker levels in TSOD mice were significantly higher 
      than those in the control mice at both ages, but there was no significant 
      difference in bone formation markers between the groups. Bone mass in TSOD mice 
      was lower than that in controls at both ages. The trabecular bone volume at the 
      femoral greater trochanter increased with age in the TSOD mice. The femoral 
      mid-diaphysis in TSOD mice was more slender and thicker than that in TSNO mice at 
      both ages. CONCLUSIONS: Bone mass of the femur was lower in TSOD mice than in 
      TSNO mice because hyperinsulinemia during pre-diabetic and established diabetic 
      conditions enhanced bone resorption due to high bone turnover. In addition, our 
      data suggest that the bone mass of the femur was significantly reduced as a 
      result of chronic hyperglycemia during established diabetic conditions in TSOD 
      mice. We suggest that bone strength in the femur deteriorated due to the 
      reduction of bone mass and because the femoral mid-diaphysis was more slender in 
      TSOD mice.
FAU - Tanaka, Hiroaki
AU  - Tanaka H
AD  - Graduate School of Health Science Suzuka University of Medical Science, 1001-1 
      Kishioka, Suzuka, Mie 510-0293, Japan.
FAU - Yamashita, Takenori
AU  - Yamashita T
AD  - Department of Radiological Technology, Faculty of Health Science, Suzuka 
      University of Medical Science, 1001-1 Kishioka, Suzuka, Mie 510-0293, Japan.
FAU - Yoneda, Misao
AU  - Yoneda M
AD  - Department of Clinical Nutrition, Faculty of Health Science, Suzuka University of 
      Medical Science, 1001-1 Kishioka, Suzuka, Mie 510-0293, Japan.
FAU - Takagi, Satoshi
AU  - Takagi S
AD  - Department of Physical Therapy, Faculty of Health and Medical Sciences, Tokoha 
      University, 1230 Miyakoda, Kitaku, Hamamatsu, Shizuoka, 431-2102, Japan.
FAU - Miura, Toshihiro
AU  - Miura T
AD  - Graduate School of Health Science Suzuka University of Medical Science, 1001-1 
      Kishioka, Suzuka, Mie 510-0293, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180721
PL  - United States
TA  - Bone Rep
JT  - Bone reports
JID - 101646176
PMC - PMC6073051
OTO - NOTNLM
OT  - BMC, bone mineral content
OT  - BMD, bone mineral density
OT  - Bone mass
OT  - Bone metabolism
OT  - Bone microstructure
OT  - Bone strength
OT  - CSMI, cross-sectional moment inertia
OT  - OCN, osteocalcin
OT  - OGTT, oral glucose tolerance test
OT  - PBS, phosphate-buffered saline
OT  - T1DM, type 1 diabetes mellitus
OT  - T2DM, type 2 diabetes mellitus
OT  - TRAcP5b, tartrate-resistant acid phosphatase 5b
OT  - TSNO, Tsumura, Suzuki, non-obesity
OT  - TSOD, Tsumura, Suzuki, Obese Diabetes
OT  - Tsumura, Suzuki, Obese Diabetes mice
OT  - Type 2 diabetes mellitus
OT  - micro-CT, micro-computed tomography
EDAT- 2018/08/11 06:00
MHDA- 2018/08/11 06:01
PMCR- 2018/07/21
CRDT- 2018/08/11 06:00
PHST- 2017/10/02 00:00 [received]
PHST- 2018/07/13 00:00 [revised]
PHST- 2018/07/19 00:00 [accepted]
PHST- 2018/08/11 06:00 [entrez]
PHST- 2018/08/11 06:00 [pubmed]
PHST- 2018/08/11 06:01 [medline]
PHST- 2018/07/21 00:00 [pmc-release]
AID - S2352-1872(18)30045-7 [pii]
AID - 10.1016/j.bonr.2018.07.004 [doi]
PST - epublish
SO  - Bone Rep. 2018 Jul 21;9:74-83. doi: 10.1016/j.bonr.2018.07.004. eCollection 2018 
      Dec.