PMID- 3009590
OWN - NLM
STAT- MEDLINE
DCOM- 19860616
LR  - 20190723
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 77
IP  - 5
DP  - 1986 May
TI  - Studies on the biosynthesis of leukotrienes in guinea pig lung in vitro by use of 
      high-performance liquid chromatography.
PG  - 720-7
AB  - The biosynthesis of leukotrienes (LTs) and 
      5-hydroxy-6,8,-11,14(E,Z,Z,Z)-eicosatetraenoic acid (5-HETE) in perfused guinea 
      pig lung was studied by reversed phase-high-performance liquid chromatography 
      (RP-HPLC). Perfusion of lungs with a 20 microM solution of ionophore A23187 for 
      25 minutes induced a sustained release of 
      5S-hydroxy-6R-S-cysteinylglycyl-7,9,11,14-(E,E,Z,Z)-eicosatetraenoic acid (LTD4) 
      and 5S,12R-dihydroxy-6,8,10,14(Z,E,E,Z)-eicosatetraenoic acid (LTB4). The 
      perfusion of 60 microM of arachidonic acid together with the ionophore only 
      slightly increased the synthesis of LTD4 and LTB4 in comparison with the effect 
      of the ionophore alone but markedly stimulated the release of 5-HETE that was not 
      detectable under other experimental conditions. Immunologic challenge of the lung 
      by continuous perfusion of ovalbumin to previously sensitized guinea pigs induced 
      the release of 50 to 150 pM of LTB4 and 15 to 50 pM of LTD4 during a 25-minute 
      period. Antigen was a less potent stimulus than the ionophore that caused the 
      release of 500 to 900 pM of LTB4 and 100 to 250 pM of LTD4 during 25-minutes of 
      perfusion. None of the above-mentioned metabolites were detected when lungs were 
      perfused with arachidonic acid alone, indicating that the synthesis of 
      5-lipoxygenase products required activation of the lung. In addition to 
      5-lipoxygenase products, the cyclooxygenase products 
      12-hydroxy-5,8,10-(Z,E,E)-heptadecatrienoic acid and 
      12-keto-5,8,10-(Z,E,E)-heptadecatrienoic acid were analyzed by RP-HPLC in guinea 
      pig lung perfusates. HPLC analysis provided quantitative data on the release of 
      several arachidonic acid metabolites by lungs.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Salari, H
AU  - Salari H
FAU - Borgeat, P
AU  - Borgeat P
FAU - Braquet, P
AU  - Braquet P
FAU - Sirois, P
AU  - Sirois P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (SRS-A)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 37H9VM9WZL (Calcimycin)
SB  - IM
MH  - Animals
MH  - Calcimycin/pharmacology
MH  - Chromatography, High Pressure Liquid
MH  - Female
MH  - Guinea Pigs
MH  - Leukotriene B4/*biosynthesis
MH  - Lung/drug effects/*metabolism
MH  - Male
MH  - SRS-A/*biosynthesis
MH  - Time Factors
EDAT- 1986/05/01 00:00
MHDA- 1986/05/01 00:01
CRDT- 1986/05/01 00:00
PHST- 1986/05/01 00:00 [pubmed]
PHST- 1986/05/01 00:01 [medline]
PHST- 1986/05/01 00:00 [entrez]
AID - 0091-6749(86)90417-3 [pii]
AID - 10.1016/0091-6749(86)90417-3 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 1986 May;77(5):720-7. doi: 10.1016/0091-6749(86)90417-3.