PMID- 30096391
OWN - NLM
STAT- MEDLINE
DCOM- 20200316
LR  - 20210504
IS  - 1097-6825 (Electronic)
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 143
IP  - 3
DP  - 2019 Mar
TI  - B lymphocyte-induced maturation protein 1 controls T(H)9 cell development, IL-9 
      production, and allergic inflammation.
PG  - 1119-1130.e3
LID - S0091-6749(18)31131-X [pii]
LID - 10.1016/j.jaci.2018.06.046 [doi]
AB  - BACKGROUND: The transcriptional repressor B lymphocyte-induced maturation protein 
      1 (Blimp-1) has a key role in terminal differentiation in various T-cell 
      subtypes. However, whether Blimp-1 regulates T(H)9 differentiation and its role 
      in allergic inflammation are unknown. OBJECTIVE: We aimed to investigate the role 
      of Blimp-1 in T(H)9 differentiation and in the pathogenesis of allergic airway 
      inflammation. METHODS: In vitro T(H)9 differentiation, flow cytometry, ELISA, and 
      real-time PCR were used to investigate the effects of Blimp-1 on T(H)9 
      polarization. T cell-specific Blimp-1-deficient mice, a model of allergic airway 
      inflammation, and T-cell adoptive transfer to recombination-activating gene 1 
      (Rag-1)(-/-) mice were used to address the role of Blimp-1 in the pathogenesis of 
      allergic inflammation. RESULTS: We found that Blimp-1 regulates T(H)9 
      differentiation because deleting Blimp-1 increased IL-9 production in CD4(+) T 
      cells in vitro. In addition, we showed that in T cell-specific Blimp-1-deficient 
      mice, deletion of Blimp-1 in T cells worsened airway disease, and this worsening 
      was inhibited by IL-9 neutralization. In asthmatic patients CD4(+) T cells in 
      response to TGF-beta plus IL-4 increased IL-9 expression and downregulated Blimp-1 
      expression compared with expression in healthy control subjects. Blimp-1 
      overexpression in human T(H)9 cells inhibited IL-9 expression. CONCLUSION: 
      Blimp-1 is a pivotal negative regulator of T(H)9 differentiation and controls 
      allergic inflammation.
CI  - Copyright (c) 2018 American Academy of Allergy, Asthma & Immunology. Published by 
      Elsevier Inc. All rights reserved.
FAU - Benevides, Luciana
AU  - Benevides L
AD  - Department of Biochemistry and Immunology, Ribeirao Preto Medical School 
      University of Sao Paulo, Ribeirao Preto, Brazil.
FAU - Costa, Renata Sesti
AU  - Costa RS
AD  - Department of Biochemistry and Immunology, Ribeirao Preto Medical School 
      University of Sao Paulo, Ribeirao Preto, Brazil.
FAU - Tavares, Lucas Alves
AU  - Tavares LA
AD  - Department of Cellular and Molecular Biology, Ribeirao Preto Medical School 
      University of Sao Paulo, Ribeirao Preto, Brazil.
FAU - Russo, Momtchilo
AU  - Russo M
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo.
FAU - Martins, Gislaine A
AU  - Martins GA
AD  - F. Widjaja Foundation Inflammatory Bowel and Immunobiology Research Institute and 
      Department of Medicine and Biomedical Science, Cedars-Sinai Medical Center 
      (CSMC), Los Angeles, Calif.
FAU - da Silva, Luis Lamberti P
AU  - da Silva LLP
AD  - Department of Cellular and Molecular Biology, Ribeirao Preto Medical School 
      University of Sao Paulo, Ribeirao Preto, Brazil.
FAU - Arruda, L Karla
AU  - Arruda LK
AD  - Department of Clinical Medicine, Clinical Hospital of Ribeirao Preto Medical 
      School, University of Sao Paulo, Ribeirao Preto, Brazil.
FAU - Cunha, Fernando Q
AU  - Cunha FQ
AD  - Department of Pharmacology, Ribeirao Preto Medical School University of Sao 
      Paulo, Ribeirao Preto, Brazil.
FAU - Carregaro, Vanessa
AU  - Carregaro V
AD  - Department of Biochemistry and Immunology, Ribeirao Preto Medical School 
      University of Sao Paulo, Ribeirao Preto, Brazil.
FAU - Silva, Joao Santana
AU  - Silva JS
AD  - Department of Biochemistry and Immunology, Ribeirao Preto Medical School 
      University of Sao Paulo, Ribeirao Preto, Brazil; Fiocruz-Bi-Institutional 
      Translational Medicine Platform, Ribeirao Preto, Brazil. Electronic address: 
      jsdsilva@fmrp.usp.br.
LA  - eng
GR  - R01 AI103542/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180807
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Interleukin-9)
RN  - EC 2.1.1.- (Positive Regulatory Domain I-Binding Factor 1)
SB  - IM
CIN - J Allergy Clin Immunol. 2019 Mar;143(3):937-939. PMID: 30193841
MH  - Animals
MH  - Asthma/*immunology
MH  - *Cell Differentiation
MH  - Cell Line
MH  - Humans
MH  - Inflammation/immunology
MH  - Interleukin-9/genetics/*immunology
MH  - Mice, Transgenic
MH  - Positive Regulatory Domain I-Binding Factor 1/*physiology
MH  - T-Lymphocytes, Helper-Inducer/*physiology
PMC - PMC8091629
MID - NIHMS1694653
OTO - NOTNLM
OT  - Allergy
OT  - Blimp-1
OT  - IL-9
OT  - T(H)9 differentiation
COIS- Disclosure of potential conflict of interest: The authors have nothing disclose.
EDAT- 2018/08/11 06:00
MHDA- 2020/03/17 06:00
PMCR- 2021/05/03
CRDT- 2018/08/11 06:00
PHST- 2017/11/16 00:00 [received]
PHST- 2018/05/08 00:00 [revised]
PHST- 2018/06/29 00:00 [accepted]
PHST- 2018/08/11 06:00 [pubmed]
PHST- 2020/03/17 06:00 [medline]
PHST- 2018/08/11 06:00 [entrez]
PHST- 2021/05/03 00:00 [pmc-release]
AID - S0091-6749(18)31131-X [pii]
AID - 10.1016/j.jaci.2018.06.046 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2019 Mar;143(3):1119-1130.e3. doi: 
      10.1016/j.jaci.2018.06.046. Epub 2018 Aug 7.