PMID- 3009695 OWN - NLM STAT- MEDLINE DCOM- 19860619 LR - 20071114 IS - 0022-1317 (Print) IS - 0022-1317 (Linking) VI - 67 ( Pt 5) DP - 1986 May TI - Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10. PG - 957-62 AB - The complete sequence of the RNA segment that codes for a major outer capsid protein (VP5) of bluetongue virus serotype 10 has been determined from overlapping cDNA clones inserted into pBR322. The segment 5 RNA of the virus (M5 RNA) is deduced to be 1638 base pairs long (1.05 X 10(6) daltons) and has an open reading frame in one strand capable of coding for a protein with a calculated size of 59 163 daltons (526 amino acids) and a net charge of -4.5 at neutral pH. FAU - Purdy, M A AU - Purdy MA FAU - Ritter, G D AU - Ritter GD FAU - Roy, P AU - Roy P LA - eng GR - CA 09128/CA/NCI NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 RN - 0 (Amino Acids) RN - 0 (Viral Proteins) RN - 0 (Viral Structural Proteins) RN - 9007-49-2 (DNA) RN - EC 3.1.21.- (DNA Restriction Enzymes) SB - IM MH - Amino Acids/analysis MH - Animals MH - Base Sequence MH - Bluetongue virus/classification/*genetics MH - *Cloning, Molecular MH - DNA/*analysis MH - DNA Restriction Enzymes MH - *Genes MH - *Genes, Viral MH - Reoviridae/*genetics MH - Serotyping MH - Viral Proteins/*genetics MH - Viral Structural Proteins EDAT- 1986/05/01 00:00 MHDA- 1986/05/01 00:01 CRDT- 1986/05/01 00:00 PHST- 1986/05/01 00:00 [pubmed] PHST- 1986/05/01 00:01 [medline] PHST- 1986/05/01 00:00 [entrez] AID - 10.1099/0022-1317-67-5-957 [doi] PST - ppublish SO - J Gen Virol. 1986 May;67 ( Pt 5):957-62. doi: 10.1099/0022-1317-67-5-957.