PMID- 30097755
OWN - NLM
STAT- MEDLINE
DCOM- 20190107
LR  - 20190107
IS  - 1573-7446 (Electronic)
IS  - 0165-7380 (Linking)
VI  - 42
IP  - 4
DP  - 2018 Dec
TI  - Triggers of NLRC4 and AIM2 inflammasomes induce porcine IL-1beta secretion.
PG  - 265-273
LID - 10.1007/s11259-018-9729-x [doi]
AB  - Pigs are an important livestock and serve as a large animal model due to 
      physiological and anatomical similarities with humans. Thus, components of the 
      porcine immune system such as inflammasomes need to be characterized for disease 
      control, vaccination, and translational research purposes. Previously, we and 
      others elucidated porcine nucleotide-binding oligomerization domain (NOD)-like 
      receptor (NLR) family Pyrin domain containing 3 (NLRP3) inflammasome activation. 
      However, until now, porcine NLR family caspase recruitment domain 
      (CARD)-containing 4 (NLRC4) and absent in melanoma 2 (AIM2) inflammasomes have 
      been not well studied. In this study, we treated well defined NLRC4 and AIM2 
      inflammasome triggers to porcine peripheral blood mononuclear cells (PBMCs) and 
      murine bone-marrow derived macrophages (BMDMs) and observed interleukin (IL)-1beta 
      maturation as a readout of inflammasome activation. NLRC4 (flagellin) and AIM2 
      (dsDNA) triggers led to IL-1beta secretion in both porcine PBMCs and mice 
      macrophages. In addition, porcine and mouse NLRC4 and AIM2 inflammasomes 
      responded differently to NLRP3 inhibitors. Bacterial inflammasome triggers, 
      Salmonella enterica serovar Typhimurium, Listeria monocytogenes, and Escherichia 
      coli, also induced IL-1beta secretion in porcine PBMCs. Taken together, we suggest 
      that known triggers of NLRC4 and AIM2 inflammasomes in mice induce IL-1beta 
      secretion in porcine PBMCs.
FAU - Ahn, Huijeong
AU  - Ahn H
AD  - Lab. of Inflammatory Diseases, Department of Physiology, College of Veterinary 
      Medicine and Institute of Veterinary Science, Kangwon National University, 
      Chuncheon, 24341, Republic of Korea.
FAU - Kim, Jeongeun
AU  - Kim J
AD  - Lab. of Inflammatory Diseases, Department of Physiology, College of Veterinary 
      Medicine and Institute of Veterinary Science, Kangwon National University, 
      Chuncheon, 24341, Republic of Korea.
FAU - Kwon, Sungkyun
AU  - Kwon S
AD  - Lab. of Inflammatory Diseases, Department of Physiology, College of Veterinary 
      Medicine and Institute of Veterinary Science, Kangwon National University, 
      Chuncheon, 24341, Republic of Korea.
AD  - Sunjin Bridge Lab Co., Ltd., Incheon, Kyoungki, 17334, Republic of Korea.
FAU - Kim, Pyeung-Hyeun
AU  - Kim PH
AD  - Department of Molecular Bioscience, School of Biomedical Science, Kangwon 
      National University, Chuncheon, 24341, Republic of Korea.
FAU - Kwon, Hyuk Moo
AU  - Kwon HM
AD  - Lab. of Inflammatory Diseases, Department of Physiology, College of Veterinary 
      Medicine and Institute of Veterinary Science, Kangwon National University, 
      Chuncheon, 24341, Republic of Korea.
FAU - Lee, Eunsong
AU  - Lee E
AD  - Lab. of Inflammatory Diseases, Department of Physiology, College of Veterinary 
      Medicine and Institute of Veterinary Science, Kangwon National University, 
      Chuncheon, 24341, Republic of Korea.
FAU - Lee, Geun-Shik
AU  - Lee GS
AUID- ORCID: 0000-0002-3149-8433
AD  - Lab. of Inflammatory Diseases, Department of Physiology, College of Veterinary 
      Medicine and Institute of Veterinary Science, Kangwon National University, 
      Chuncheon, 24341, Republic of Korea. leegeun@kangwon.ac.kr.
LA  - eng
GR  - NRF-2015R1A2A2A01004183/National Research Foundation of Korea/
GR  - NRF-2016R1A4A1010115/National Research Foundation of Korea/
GR  - NRF-2018R1A2B2004097/National Research Foundation of Korea/
GR  - 520160446/2016 Research Grant from Kangwon National University/
PT  - Journal Article
DEP - 20180810
PL  - Switzerland
TA  - Vet Res Commun
JT  - Veterinary research communications
JID - 8100520
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Nod Signaling Adaptor Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - DNA-Binding Proteins/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Inflammasomes/drug effects/*metabolism
MH  - Interleukin-1beta/*metabolism
MH  - Leukocytes, Mononuclear/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nod Signaling Adaptor Proteins/*metabolism
MH  - Salmonella Infections, Animal/immunology/metabolism
MH  - Swine
MH  - Swine Diseases/immunology/metabolism/microbiology
OTO - NOTNLM
OT  - AIM2
OT  - Interleukin-1beta
OT  - Macrophages
OT  - NLRC4
OT  - Pig
EDAT- 2018/08/12 06:00
MHDA- 2019/01/08 06:00
CRDT- 2018/08/12 06:00
PHST- 2018/01/29 00:00 [received]
PHST- 2018/07/19 00:00 [accepted]
PHST- 2018/08/12 06:00 [pubmed]
PHST- 2019/01/08 06:00 [medline]
PHST- 2018/08/12 06:00 [entrez]
AID - 10.1007/s11259-018-9729-x [pii]
AID - 10.1007/s11259-018-9729-x [doi]
PST - ppublish
SO  - Vet Res Commun. 2018 Dec;42(4):265-273. doi: 10.1007/s11259-018-9729-x. Epub 2018 
      Aug 10.