PMID- 30100091
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1532-2785 (Electronic)
IS  - 0143-4179 (Linking)
VI  - 71
DP  - 2018 Oct
TI  - Up-regulation of TNF Receptor-associated Factor 7 after spinal cord injury in 
      rats may have implication for neuronal apoptosis.
PG  - 81-89
LID - S0143-4179(18)30061-1 [pii]
LID - 10.1016/j.npep.2018.08.001 [doi]
AB  - TNF receptor-associated factor 7 (TRAF7), is an E3 ubiquitin ligase for several 
      proteins involved in the activation of TLR-dependent NF-kappaB signaling. TRAF7 
      links TNF receptor family proteins to signaling pathways, thus participates in 
      regulating cell death and survival mediated by TNF family ligands. To date, the 
      biological function of TRAF7 after spinal cord injury (SCI) is still with limited 
      acquaintance. In this study, we have performed an acute SCI model in adult rats 
      and investigated the dynamic changes of TRAF7 expression in the spinal cord. Our 
      results showed that TRAF7 was up-regulated significantly after SCI, which was 
      paralleled with the levels of the apoptotic protein active caspase-3. 
      Immunofluorescent labeling showed that TRAF7 was co-localizated with active 
      caspase-3 in neurons. To further investigate the function of TRAF7, an apoptosis 
      model was established in primary neuronal cells. When TRAF7 was knocked down by 
      specific short interfering RNA (siRNA), the protein levels of active caspase-3 
      and the number of apoptotic primary neurons were significantly decreased in our 
      study. Taken together, our findings suggest that the change of TRAF7 protein 
      expression plays a key role in neuronal apoptosis after SCI.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Xu, Dawei
AU  - Xu D
AD  - Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, 
      Nantong 226001, People's Republic of China.
FAU - Zhao, Wei
AU  - Zhao W
AD  - Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, 
      Nantong 226001, People's Republic of China.
FAU - Wang, Chengniu
AU  - Wang C
AD  - Basic Medical Research Centre, Medical College, Nantong University, Nantong 
      226001, People's Republic of China.
FAU - Zhu, Hao
AU  - Zhu H
AD  - Department of Orthopaedics, The Fourth Affiliated Hospital of Nantong University, 
      Yancheng 224005, People's Republic of China.
FAU - He, Mingqing
AU  - He M
AD  - Department of Geriatrics, The First Affiliated Hospital of Soochow University, 
      Suzhou 215006, People's Republic of China.
FAU - Zhu, Xinhui
AU  - Zhu X
AD  - Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, 
      Nantong 226001, People's Republic of China.
FAU - Liu, Wei
AU  - Liu W
AD  - Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, 
      Nantong 226001, People's Republic of China.
FAU - Wang, Fei
AU  - Wang F
AD  - Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, 
      Nantong 226001, People's Republic of China.
FAU - Fan, Jianbo
AU  - Fan J
AD  - Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, 
      Nantong 226001, People's Republic of China.
FAU - Chen, Chu
AU  - Chen C
AD  - Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, 
      Nantong 226001, People's Republic of China.
FAU - Cui, Daoran
AU  - Cui D
AD  - Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, 
      Nantong 226001, People's Republic of China.
FAU - Cui, Zhiming
AU  - Cui Z
AD  - Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, 
      Nantong 226001, People's Republic of China. Electronic address: 
      zhimingcui_orth1@163.com.
LA  - eng
PT  - Journal Article
DEP - 20180808
PL  - Netherlands
TA  - Neuropeptides
JT  - Neuropeptides
JID - 8103156
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor Receptor-Associated Peptides and Proteins)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Behavior, Animal/physiology
MH  - Disease Models, Animal
MH  - Male
MH  - Motor Activity/physiology
MH  - Neurons/*metabolism
MH  - RNA, Small Interfering
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/*metabolism
MH  - Spinal Cord Injuries/genetics/*metabolism
MH  - Tumor Necrosis Factor Receptor-Associated Peptides and 
      Proteins/genetics/*metabolism
MH  - *Up-Regulation
OTO - NOTNLM
OT  - Neuronal apoptosis
OT  - Rats
OT  - Spinal cord injury
OT  - TNF receptor-associated factor 7
EDAT- 2018/08/14 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/08/14 06:00
PHST- 2018/03/22 00:00 [received]
PHST- 2018/07/05 00:00 [revised]
PHST- 2018/08/07 00:00 [accepted]
PHST- 2018/08/14 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/08/14 06:00 [entrez]
AID - S0143-4179(18)30061-1 [pii]
AID - 10.1016/j.npep.2018.08.001 [doi]
PST - ppublish
SO  - Neuropeptides. 2018 Oct;71:81-89. doi: 10.1016/j.npep.2018.08.001. Epub 2018 Aug 
      8.