PMID- 30102527
OWN - NLM
STAT- MEDLINE
DCOM- 20180914
LR  - 20211204
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 66
IP  - 35
DP  - 2018 Sep 5
TI  - Biotin-Modified Polylactic- co-Glycolic Acid Nanoparticles with Improved 
      Antiproliferative Activity of 15,16-Dihydrotanshinone I in Human Cervical Cancer 
      Cells.
PG  - 9219-9230
LID - 10.1021/acs.jafc.8b02698 [doi]
AB  - 15,16-Dihydrotanshinone I (DI), a natural compound isolated from a traditional 
      Asian functional food Salvia Miltiorrhiza Bunge, is known for its anticancer 
      activity. However, poor solubility of DI limits its desirable anticancer 
      application. Herein, polylactic- co-glycolic acid (PLGA) was functionalized with 
      polyethylene glycol (PEG) and biotin to form copolymers PEG-PLGA (PPA) and 
      biotin-PEG-PLGA (BPA). DI was encapsulated in copolymers PPA and BPA to obtain 
      DI-PPA-NPs (NPs = nanoparticles) and DI-BPA-NPs, respectively. The particle size 
      and its distribution, encapsulation efficiency, and in vitro releasing capacity 
      of DI-BPA-NPs were characterized by biophysical methods. MTT assay was used to 
      evaluate the antiproliferative activity of free DI, DI-PPA-NPs, and DI-BPA-NPs in 
      human cervical cancer Hela cells. DI-BPA-NPs showed the highest cytotoxicity on 
      Hela cells with an IC(50) value of 4.55 +/- 0.631 muM, while it was 8.20 +/- 0.849 and 
      6.14 +/- 0.312 muM for DI and DI-PPA-NPs in 72 h, respectively. The superior 
      antiproliferative activity was supported by the fact that DI-BPA-NPs could be 
      preferentially internalized by Hela cells, owing to their specific interaction 
      between biotin and overexpressed biotin receptors. In addition, DI-BPA-NPs 
      effectively inhibited Hela cell proliferation by inducing G2/M phase cycle arrest 
      and decreasing the intracellular reactive oxygen species (ROS) level by 31.50 +/- 
      2.29% in 5 min. In summary, DI-BPA-NPs shows improved antiproliferative activity 
      against human cervical cancer as comparing with free DI, demonstrating its 
      application potential in cancer therapy.
FAU - Luo, Jingjing
AU  - Luo J
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
AD  - Guangdong Province Key Laboratory for Green Processing of Natural Products and 
      Product Safety , Guangzhou 510640 , China.
FAU - Meng, Xiaofeng
AU  - Meng X
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
AD  - Guangdong Province Key Laboratory for Green Processing of Natural Products and 
      Product Safety , Guangzhou 510640 , China.
FAU - Su, Jianyu
AU  - Su J
AUID- ORCID: 0000-0002-1662-0461
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
AD  - Guangdong Province Key Laboratory for Green Processing of Natural Products and 
      Product Safety , Guangzhou 510640 , China.
AD  - Overseas Expertise Introduction Center for Discipline Innovation of Food 
      Nutrition and Human Health (111 Center) , Guangzhou 510640 , China.
FAU - Ma, Hang
AU  - Ma H
AUID- ORCID: 0000-0001-7565-6889
AD  - Bioactive Botanical Research Laboratory, Department of Biomedical and 
      Pharmaceutical Sciences, College of Pharmacy , University of Rhode Island , 
      Kingston , Rhode Island 02881 , United States.
FAU - Wang, Wen
AU  - Wang W
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
AD  - Guangdong Province Key Laboratory for Green Processing of Natural Products and 
      Product Safety , Guangzhou 510640 , China.
FAU - Fang, Liming
AU  - Fang L
AD  - Department of Materials Science and Engineering , South China University of 
      Technology , Guangzhou 510640 , China.
FAU - Zheng, Huade
AU  - Zheng H
AD  - Department of Materials Science and Engineering , South China University of 
      Technology , Guangzhou 510640 , China.
FAU - Qin, Yexia
AU  - Qin Y
AD  - Institute of Industrial Technology Research , South China University of 
      Technology , Guangzhou 510640 , China.
FAU - Chen, Tianfeng
AU  - Chen T
AUID- ORCID: 0000-0001-6953-1342
AD  - Department of Chemistry , Jinan University , Guangzhou , 510632 , China.
LA  - eng
PT  - Journal Article
DEP - 20180822
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Drug Carriers)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Furans)
RN  - 0 (Glycolates)
RN  - 0 (Phenanthrenes)
RN  - 0 (Polyesters)
RN  - 0 (Quinones)
RN  - 0 (Reactive Oxygen Species)
RN  - 0WT12SX38S (glycolic acid)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 562G9360V6 (dihydrotanshinone I)
RN  - 6SO6U10H04 (Biotin)
SB  - IM
MH  - Antineoplastic Agents/*chemistry/*pharmacology
MH  - Biotin/chemistry
MH  - Cell Cycle/drug effects
MH  - Cell Proliferation/drug effects
MH  - Drug Carriers/chemical synthesis/chemistry
MH  - Drug Compounding
MH  - Drug Delivery Systems
MH  - Drugs, Chinese Herbal/*chemistry/*pharmacology
MH  - Female
MH  - Furans
MH  - Glycolates/chemistry
MH  - HeLa Cells
MH  - Humans
MH  - Particle Size
MH  - Phenanthrenes/*chemistry/*pharmacology
MH  - Polyesters/chemistry
MH  - Quinones
MH  - Reactive Oxygen Species/metabolism
MH  - Salvia miltiorrhiza/*chemistry
MH  - Uterine Cervical Neoplasms/*drug therapy/metabolism/physiopathology
OTO - NOTNLM
OT  - 15,16-dihydrotanshinone I
OT  - PLGA nanoparticles
OT  - antiproliferative
OT  - biotin
OT  - cervical cancer
OT  - targeted delivery
EDAT- 2018/08/14 06:00
MHDA- 2018/09/15 06:00
CRDT- 2018/08/14 06:00
PHST- 2018/08/14 06:00 [pubmed]
PHST- 2018/09/15 06:00 [medline]
PHST- 2018/08/14 06:00 [entrez]
AID - 10.1021/acs.jafc.8b02698 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2018 Sep 5;66(35):9219-9230. doi: 10.1021/acs.jafc.8b02698. 
      Epub 2018 Aug 22.