PMID- 30104180
OWN - NLM
STAT- MEDLINE
DCOM- 20190122
LR  - 20210109
IS  - 2352-3964 (Electronic)
IS  - 2352-3964 (Linking)
VI  - 35
DP  - 2018 Sep
TI  - Circulating tumor cells detection in neuroblastoma patients by EpCAM-independent 
      enrichment and immunostaining-fluorescence in situ hybridization.
PG  - 244-250
LID - S2352-3964(18)30288-3 [pii]
LID - 10.1016/j.ebiom.2018.08.005 [doi]
AB  - Detecting circulating tumor cells (CTCs) has proven valuable for evaluating the 
      prognosis of cancer patients and for studying the mechanisms of treatment 
      resistance. Owing to the lack of universal and specific tumor markers for 
      neuroblastoma (NB), in this prospective study, we adopted an EpCAM-independent 
      method to detect CTCs in the peripheral blood of NB patients. We used an 
      EpCAM-independent assay to delete leukocytes and to enrich the CTCs. CTCs were 
      identified by immunostaining of CD45, DAPI and DNA fluorescence in situ 
      hybridization (FISH) of the centromere of chromosome 8 probe (CEP8). Cells that 
      were DAPI+/CD45-/CEP8 >/= 3 were considered CTCs. We collected peripheral blood 
      from 28 NB patients as well as clinical and follow-up data. The number of CTCs 
      among the different risk groups were significantly different (p = .0208, 
      Kruskal-Wallis test). Patients with metastasis had more CTCs than those without 
      metastasis (p < .0001, Mann-Whitney test). Patients with >/=3 CTCs per 4 ml of 
      peripheral blood had an increased likelihood of having metastasis (sensitivity, 
      88.89%; specificity, 78.59%), and patients with >/=10 CTCs per 4 ml of peripheral 
      blood had poorer overall survival. The EpCAM-independent assay along with 
      immunostaining-FISH (i-FISH) described here can detect CTCs in patients with NB 
      at a high sensitivity and may have clinical value for prognosis evaluation and 
      diagnosing metastasis when imaging data are ambiguous.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - Liu, Xiangqi
AU  - Liu X
AD  - Department of Pediatric Surgery, Children's Hospital of Fudan University, 
      Shanghai Key Laboratory of Birth Defect, Key Laboratory of Neonatal Disease, 
      Ministry of Health, 399 Wan Yuan Road, Shanghai 201102, China.
FAU - Zhang, Zhenzhen
AU  - Zhang Z
AD  - Biotecan Medical Diagnostics Co., Ltd, Zhangjiang Center for Translational 
      Medicine, Shanghai, China.
FAU - Zhang, Binbin
AU  - Zhang B
AD  - Department of Pediatric Surgery, Children's Hospital of Fudan University, 
      Shanghai Key Laboratory of Birth Defect, Key Laboratory of Neonatal Disease, 
      Ministry of Health, 399 Wan Yuan Road, Shanghai 201102, China.
FAU - Zheng, Yijie
AU  - Zheng Y
AD  - Medical Scientific Liaison Asian Pacific, Abbott Diagnostics Division, Abbott 
      Laboratories, Shanghai, China.
FAU - Zheng, Chao
AU  - Zheng C
AD  - Department of Pediatric Surgery, Children's Hospital of Fudan University, 
      Shanghai Key Laboratory of Birth Defect, Key Laboratory of Neonatal Disease, 
      Ministry of Health, 399 Wan Yuan Road, Shanghai 201102, China.
FAU - Liu, Baihui
AU  - Liu B
AD  - Department of Pediatric Surgery, Children's Hospital of Fudan University, 
      Shanghai Key Laboratory of Birth Defect, Key Laboratory of Neonatal Disease, 
      Ministry of Health, 399 Wan Yuan Road, Shanghai 201102, China.
FAU - Zheng, Shan
AU  - Zheng S
AD  - Department of Pediatric Surgery, Children's Hospital of Fudan University, 
      Shanghai Key Laboratory of Birth Defect, Key Laboratory of Neonatal Disease, 
      Ministry of Health, 399 Wan Yuan Road, Shanghai 201102, China.
FAU - Dong, Kuiran
AU  - Dong K
AD  - Department of Pediatric Surgery, Children's Hospital of Fudan University, 
      Shanghai Key Laboratory of Birth Defect, Key Laboratory of Neonatal Disease, 
      Ministry of Health, 399 Wan Yuan Road, Shanghai 201102, China. Electronic 
      address: kuirand@fudan.edu.cn.
FAU - Dong, Rui
AU  - Dong R
AD  - Department of Pediatric Surgery, Children's Hospital of Fudan University, 
      Shanghai Key Laboratory of Birth Defect, Key Laboratory of Neonatal Disease, 
      Ministry of Health, 399 Wan Yuan Road, Shanghai 201102, China. Electronic 
      address: rdong@fudan.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180810
PL  - Netherlands
TA  - EBioMedicine
JT  - EBioMedicine
JID - 101647039
RN  - 0 (EPCAM protein, human)
RN  - 0 (Epithelial Cell Adhesion Molecule)
RN  - 0 (MYCN protein, human)
RN  - 0 (N-Myc Proto-Oncogene Protein)
RN  - 55-10-7 (Vanilmandelic Acid)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Epithelial Cell Adhesion Molecule/*metabolism
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Gene Amplification
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Logistic Models
MH  - Male
MH  - Multivariate Analysis
MH  - N-Myc Proto-Oncogene Protein/genetics
MH  - Neoplasm Metastasis
MH  - Neoplastic Cells, Circulating/*pathology
MH  - Neuroblastoma/genetics/*pathology/urine
MH  - Phosphopyruvate Hydratase/metabolism
MH  - Proportional Hazards Models
MH  - Risk Factors
MH  - Vanilmandelic Acid/urine
PMC - PMC6154868
OTO - NOTNLM
OT  - Circulating tumor cells
OT  - Metastasis
OT  - Neuroblastoma
OT  - Prognosis
EDAT- 2018/08/15 06:00
MHDA- 2019/01/23 06:00
PMCR- 2018/08/10
CRDT- 2018/08/15 06:00
PHST- 2018/04/09 00:00 [received]
PHST- 2018/08/01 00:00 [revised]
PHST- 2018/08/01 00:00 [accepted]
PHST- 2018/08/15 06:00 [pubmed]
PHST- 2019/01/23 06:00 [medline]
PHST- 2018/08/15 06:00 [entrez]
PHST- 2018/08/10 00:00 [pmc-release]
AID - S2352-3964(18)30288-3 [pii]
AID - 10.1016/j.ebiom.2018.08.005 [doi]
PST - ppublish
SO  - EBioMedicine. 2018 Sep;35:244-250. doi: 10.1016/j.ebiom.2018.08.005. Epub 2018 
      Aug 10.