PMID- 30106146
OWN - NLM
STAT- MEDLINE
DCOM- 20190109
LR  - 20240331
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 18
IP  - 4
DP  - 2018 Oct
TI  - C‑C chemokine receptor type 3 gene knockout alleviates inflammatory responses in 
      allergic rhinitis model mice by regulating the expression of eosinophil granule 
      proteins and immune factors.
PG  - 3780-3790
LID - 10.3892/mmr.2018.9380 [doi]
AB  - The present study aimed to investigate the effects of C‑C chemokine receptor 
      type 3 (CCR3) gene knockout on allergic rhinitis (AR) in mice, as well as the 
      underlying molecular mechanisms. Ovalbumin was administrated to CCR3+/+ and 
      CCR3‑/‑ BALB/c mice to establish an AR model. The mice were divided into four 
      groups: i) Normal control (CG), ii) AR model (AR), iii) CCR3 knockout CG 
      (CCR3‑/‑CG) and iv) AR model with CCR3 knockout (CCR3‑/‑AR). Histological 
      sections of nasal mucosae were examined by hematoxylin and eosin staining, which 
      revealed that CCR3 knockout suppressed the invasion of inflammatory cells and 
      relieved the damage of nasal mucosae. Peripheral blood smear and nasal‑washing 
      smears were evaluated by Wright's staining. Eosinophil (EOS) numbers in nasal 
      mucosae, peripheral blood, and nasal washings of the various groups were ranked 
      in the order: AR>CCR3‑/‑AR>CG>CCR3‑/‑. mRNA expression levels of CCR3, EOS 
      peroxidase (EPO), EOS cationic protein (ECP), and major basic protein (MBP) in 
      the peripheral serum and nasal washings were detected by reverse 
      transcription‑polymerase chain reaction. Interferon‑gamma (IFN‑gamma), interleukin 
      (IL)‑4, IL‑10, and immunoglobulin E (IgE) protein levels in the peripheral serum 
      and nasal washings were investigated by ELISA. CCR3 mRNA expression was not 
      detected in the CCR3‑/‑ and CCR3‑/‑AR groups, whereas expression levels in the AR 
      group were markedly higher compared with expression in the CG group. Compared 
      with the CG‑associated groups (i.e., the CG and CCR3‑/‑CG groups), the levels of 
      EPO, ECP, MBP, IL‑4, and IgE were significantly increased in the AR‑associated 
      groups (that is, R and CCR3‑/‑AR). In addition, the CCR3‑/‑AR group mice produced 
      significantly lower levels of EPO, ECP, MBP, IL‑4 and IgE compared with the AR 
      group, whereas the expression levels of IFN‑gamma and IL‑10 were increased. CCR3 gene 
      knockout may alleviate EOS invasion and the inflammatory response in AR model 
      mice by reducing the expression levels of EPO, ECP, MBP, IL‑4, and IgE, and 
      increasing the expression of IL‑10 and IFN‑gamma.
FAU - Zhu, Xinhua
AU  - Zhu X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
FAU - Liu, Ke
AU  - Liu K
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
FAU - Wang, Jialin
AU  - Wang J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
FAU - Peng, Haisen
AU  - Peng H
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
FAU - Pan, Qibin
AU  - Pan Q
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
FAU - Wu, Shuhong
AU  - Wu S
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
FAU - Jiang, Yinli
AU  - Jiang Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
FAU - Liu, Yuehui
AU  - Liu Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180810
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Ccr3 protein, mouse)
RN  - 0 (Eosinophil Granule Proteins)
RN  - 0 (Immunologic Factors)
RN  - 0 (Receptors, CCR3)
SB  - IM
MH  - Animals
MH  - Eosinophil Granule Proteins/genetics/*immunology
MH  - Female
MH  - Gene Expression Regulation
MH  - Gene Knockout Techniques
MH  - Immunologic Factors/genetics/*immunology
MH  - Inflammation/genetics/*immunology/pathology
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Mice, Knockout
MH  - Nasal Mucosa/immunology/metabolism/pathology
MH  - Receptors, CCR3/genetics/*immunology
MH  - Rhinitis, Allergic/genetics/*immunology/pathology
PMC - PMC6131541
EDAT- 2018/08/15 06:00
MHDA- 2019/01/10 06:00
PMCR- 2018/08/10
CRDT- 2018/08/15 06:00
PHST- 2017/04/18 00:00 [received]
PHST- 2018/03/26 00:00 [accepted]
PHST- 2018/08/15 06:00 [pubmed]
PHST- 2019/01/10 06:00 [medline]
PHST- 2018/08/15 06:00 [entrez]
PHST- 2018/08/10 00:00 [pmc-release]
AID - mmr-18-04-3780 [pii]
AID - 10.3892/mmr.2018.9380 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Oct;18(4):3780-3790. doi: 10.3892/mmr.2018.9380. Epub 2018 Aug 
      10.