PMID- 30107664
OWN - NLM
STAT- MEDLINE
DCOM- 20181003
LR  - 20190318
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 47
IP  - 8
DP  - 2018 Aug 8
TI  - [Relationship between PD-L1 protein expression and gene amplification in gastric 
      cancer tissues].
PG  - 597-602
LID - 10.3760/cma.j.issn.0529-5807.2018.08.006 [doi]
AB  - Objective: To investigate the relationship of PD-L1 protein expression and gene 
      amplification in gastric cancer and their correlation with clinicopathologic 
      factors. Methods: The cohort included 247 gastric cancer specimens with follow-up 
      data and clinicopathologic data obtained from Shanxi Cancer Hospital in 2011. 
      PD-L1 expression was detected by immunohistochemistry (IHC) and fluorescence in 
      situ hybridization (FISH). Results: PD-L1 protein was expressed in 25.9% (64/247) 
      of the tumor cells and 26.7% (66/247) of the tumor infiltrating immune cells 
      (IC). There was a correlation between the two (P<0.01). The expression of PD-L1 
      in tumor cells correlated with the degree of differentiation and tumor 
      diameter(P<0.05). The PD-L1 expression in IC correlated with vascular tumor 
      thrombi(P<0.05). The amplification rate of PD-L1 gene detected by FISH was 19.0% 
      (47/247), and was associated with age, large/small curvature of the stomach, 
      tumor location, tumor diameter, and lymph node metastasis(P<0.05). The positive 
      coincidence rate of the two methods was 25.0% (16/64), negative coincidence rate 
      was 83.0% (152/183), and total coincidence rate was 68.0% (168/247), suggesting 
      that the coincidence of IHC and FISH was poor (P=0.157). There was a negative 
      correlation between PD-L1 protein expression on tumor cells and prognosis in 
      gastric cancer. There was no significant correlation between PD-L1 protein 
      expression on IC and PD-L1 gene amplification with prognosis. Vascular tumor 
      thrombi, tumor diameter, depth of invasion, and lymph node metastasis were all 
      poor prognostic factors of gastric cancer(P<0.05). Multivariate Cox regression 
      analysis showed that PD-L1 protein expression, depth of invasion and lymph node 
      metastasis were all independent prognostic risk factors for gastric cancer. 
      Conclusions: Concordance between PD-L1 protein expression and gene amplification 
      is poor. PD-L1 protein expression may signify poor prognosis. There is no 
      significant correlation between PD-L1 gene amplification and prognosis of 
      patients with gastric cancer.
FAU - Liu, Y
AU  - Liu Y
AD  - Department of Pathology, Shanxi Medical University, Taiyuan 030001, China.
FAU - Wang, J F
AU  - Wang JF
FAU - Li, X W
AU  - Li XW
FAU - Bu, P
AU  - Bu P
FAU - Bai, W
AU  - Bai W
FAU - Li, L M
AU  - Li LM
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (B7-H1 Antigen)
RN  - 0 (CD274 protein, human)
SB  - IM
MH  - Antigens, Neoplasm/*metabolism
MH  - B7-H1 Antigen/*metabolism
MH  - *Gene Amplification
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphatic Metastasis
MH  - Lymphocytes, Tumor-Infiltrating/*metabolism
MH  - Prognosis
MH  - Proteomics
MH  - Risk Factors
MH  - Stomach Neoplasms/*genetics/*metabolism/pathology
MH  - Tumor Burden
OTO - NOTNLM
OT  - Gene amplification
OT  - Prognosis
OT  - Programmed death-ligand 1(PD-L1)
OT  - Stomach neoplasms
EDAT- 2018/08/16 06:00
MHDA- 2018/10/04 06:00
CRDT- 2018/08/15 06:00
PHST- 2018/08/15 06:00 [entrez]
PHST- 2018/08/16 06:00 [pubmed]
PHST- 2018/10/04 06:00 [medline]
AID - 10.3760/cma.j.issn.0529-5807.2018.08.006 [doi]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2018 Aug 8;47(8):597-602. doi: 
      10.3760/cma.j.issn.0529-5807.2018.08.006.