PMID- 30111404
OWN - NLM
STAT- MEDLINE
DCOM- 20181113
LR  - 20181113
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 26
IP  - 4
DP  - 2018 Aug
TI  - [Detection of Gene Abnormalities in 43 Cases of Chronic Lymphocytic Leukemia by 
      Fluorescence in Situ Hybridization].
PG  - 1038-1043
LID - 10.7534/j.issn.1009-2137.2018.04.016 [doi]
AB  - OBJECTIVE: To explore the value of fluorescence in situ hybridization (FISH) in 
      diagnosis and prognosis evaluation of chronic lymphocytic leukemia (CLL). 
      METHODS: R banding technique was used for karyotype analysis in 43 cases of CLL, 
      and fluorescence in situ hybridization（FISH） technique was used for analysis of 5 
      genes in 43 cases of CLL, including D13S25, RB1,ATM, P53 and CEP 12 . RESULTS: 
      The detection rate of chromosome abnormality was 9.3％ for the 43 cases. The 
      chromosome karyotype abnormality was involoved in the number abnormality and the 
      structural abnormality, including No. 2, 6, 14, and sex chromosome. The patients 
      with normal chromosome karyotype were more common（79.1％）. At the same time, 
      mitotic figure in 5 CLL patients did not observed. The positive rate of FISH 
      detection was 55.8％(24/43). Among which, D13S25 deletion was the highest and 
      reached to 37.2％, followed by RB1 deletion（20.9％）, CEP 12 amplification（16.3％）, 
      ATM deletion（9.3％） and P53 deletion（7.0％）. In 24 FISH-positive cases, 20 cases 
      displayed the normal chromosome karyotype, and in 3 cases the mitotic figure was 
      absent. There was only one case of abnormal chromosome, which was not involved in 
      the positive gene tested by FISH. CONCLUSION: FISH is an important means of CLL 
      genetic detection, which can greatly raise the detection rate of cytogenetics 
      abnormalities in CLL, but the probe quantity is limited, so it is necessary to 
      use FISH technology combined with chromosome karyotype analysis so as to improve 
      the detection rate of abnormal cytogenetics, and provide the basis for the 
      clinical diagnosis and prognosis of CLL.
FAU - Jing, Yuan
AU  - Jing Y
AD  - Department of Hematology, The First Affiliated Hospital of Dalian Medical 
      University, Blood Disease MICM Key Laboratory, Dalian 116011, Liaoning Province, 
      China.
FAU - Lin, Shuang
AU  - Lin S
AD  - Department of Hematology, The First Affiliated Hospital of Dalian Medical 
      University, Blood Disease MICM Key Laboratory, Dalian 116011, Liaoning Province, 
      China.
FAU - Wang, Fang-Ting
AU  - Wang FT
AD  - Department of Hematology, The First Affiliated Hospital of Dalian Medical 
      University, Blood Disease MICM Key Laboratory, Dalian 116011, Liaoning Province, 
      China.
FAU - Yu, Jia-Wen
AU  - Yu JW
AD  - Department of Hematology, The First Affiliated Hospital of Dalian Medical 
      University, Blood Disease MICM Key Laboratory, Dalian 116011, Liaoning Province, 
      China.
FAU - Jiang, Feng
AU  - Jiang F
AD  - Department of Hematology, The First Affiliated Hospital of Dalian Medical 
      University, Blood Disease MICM Key Laboratory, Dalian 116011, Liaoning Province, 
      China.E-mail: 39989470@qq.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Chromosome Aberrations
MH  - Cytogenetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell
EDAT- 2018/08/17 06:00
MHDA- 2018/11/14 06:00
CRDT- 2018/08/17 06:00
PHST- 2018/08/17 06:00 [entrez]
PHST- 2018/08/17 06:00 [pubmed]
PHST- 2018/11/14 06:00 [medline]
AID - 1009-2137（2018）04-1038-06 [pii]
AID - 10.7534/j.issn.1009-2137.2018.04.016 [doi]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Aug;26(4):1038-1043. doi: 
      10.7534/j.issn.1009-2137.2018.04.016.