PMID- 30111866
OWN - NLM
STAT- MEDLINE
DCOM- 20191126
LR  - 20240329
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Aug 15
TI  - Stimulation of carbon nanomaterials on syntrophic oxidation of butyrate in 
      sediment enrichments and a defined coculture.
PG  - 12185
LID - 10.1038/s41598-018-30745-7 [doi]
LID - 12185
AB  - It remains elusive if direct interspecies electron transfer (DIET) occurs in 
      canonical syntrophy involving short-chain fatty acids oxidation. In the present 
      study, we determined the effects of carbon nanomaterials on syntrophic oxidation 
      of butyrate in two lake sediment enrichments and a defined coculture comprising 
      Syntrophomonas wolfei and Methanococcus Maripaludis. After four continuous 
      transfers of enrichment cultivation, Syntrophomonas dominated the bacterial 
      populations in enrichments, and the dominated methanogens comprised 
      Methanosarcina and Methanospirillum in one enrichment (from Weiming Lake) and 
      Methanoregula and Methanospirillum in another (from Erhai Lake). Butyrate 
      oxidation and CH(4) production was significantly accelerated by carbon nanotubes 
      (CNTs) in both enrichments. Replacement of CNTs by magnetite caused similar 
      stimulating effect. For the defined coculture, two carbon nanomaterials, CNTs and 
      reduced graphene oxide (rGO), were tested, both showed consistently stimulating 
      effects on butyrate oxidation. Addition of kaolinite, an electric nonconductive 
      clay mineral, however, revealed no effect. The test on M. maripaludis in pure 
      culture showed no effect by rGO and a negative effect by CNTs (especially at a 
      high concentration). Fluorescence in situ hybridization (FISH) and scanning 
      electron microscopy (SEM) revealed that microbial cells were interwoven by CNTs 
      forming cell-CNT mixture aggregates, and in case of rGO, cells were attached to 
      surface or wrapped-up by rGO thin sheets. Collectively, our data suggest that the 
      presence of conductive nanomaterials likely induces DIET in syntrophic butyrate 
      oxidation.
FAU - Zhang, Wei
AU  - Zhang W
AD  - College of Urban and Environmental Sciences, Peking University, Beijing, 100871, 
      China.
FAU - Zhang, Jianchao
AU  - Zhang J
AD  - Institute of Surface-Earth System Science, Tianjin University, Tianjin, 300072, 
      China.
FAU - Lu, Yahai
AU  - Lu Y
AUID- ORCID: 0000-0002-1702-9868
AD  - College of Urban and Environmental Sciences, Peking University, Beijing, 100871, 
      China. luyh@pku.edu.cn.
LA  - eng
GR  - 41630857/National Natural Science Foundation of China (National Science 
      Foundation of China)/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180815
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Butyrates)
RN  - 0 (Dystrophin-Associated Proteins)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (syntrophin)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Bacteria
MH  - Butyrates/*metabolism
MH  - Coculture Techniques/methods
MH  - Dystrophin-Associated Proteins/metabolism
MH  - Electron Transport
MH  - Geologic Sediments/*microbiology
MH  - Hydrogen
MH  - In Situ Hybridization, Fluorescence
MH  - Methane/biosynthesis
MH  - Nanoparticles/metabolism
MH  - Nanostructures
MH  - Nanotubes, Carbon
MH  - Oxidation-Reduction
PMC - PMC6093938
COIS- The authors declare no competing interests.
EDAT- 2018/08/17 06:00
MHDA- 2019/11/27 06:00
PMCR- 2018/08/15
CRDT- 2018/08/17 06:00
PHST- 2018/03/16 00:00 [received]
PHST- 2018/07/30 00:00 [accepted]
PHST- 2018/08/17 06:00 [entrez]
PHST- 2018/08/17 06:00 [pubmed]
PHST- 2019/11/27 06:00 [medline]
PHST- 2018/08/15 00:00 [pmc-release]
AID - 10.1038/s41598-018-30745-7 [pii]
AID - 30745 [pii]
AID - 10.1038/s41598-018-30745-7 [doi]
PST - epublish
SO  - Sci Rep. 2018 Aug 15;8(1):12185. doi: 10.1038/s41598-018-30745-7.