PMID- 3011423
OWN - NLM
STAT- MEDLINE
DCOM- 19860627
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 5
IP  - 4
DP  - 1986 Apr
TI  - A transcriptional enhancer sequence of HTLV-I is responsible for trans-activation 
      mediated by p40 chi HTLV-I.
PG  - 713-8
AB  - Human T-cell leukemia virus type I (HTLV-I) contains a unique sequence pX that is 
      located between env and the 3' long terminal repeat (LTR) and codes for three pX 
      proteins, p40 chi, pp27 chi-III and pp21 chi-III. One of these proteins, p40 chi, 
      was previously shown to activate transcription from the LTR in a trans-acting 
      manner, which suggested that it activated some cellular genes involved in 
      leukemogenesis. In this study, the sequences in the LTR responsible for this 
      trans-activation were analyzed. Construction of deletion mutants of the LTR in 
      pLTR-CAT and measurement of their activities in trans-activated expression of the 
      CAT gene showed that sequences upstream of the TATA box were responsible for the 
      trans-activation mediated by p40 chi. The active unit was identified as an 
      enhancer sequence containing direct repeats by inserting it into an 
      enhancer-minus SV40 promoter. Thus, it was concluded that an enhancer sequence in 
      HTLV-I LTR is responsible, at least in part, for transcriptional trans-activation 
      mediated by the viral product p40 chi.
FAU - Fujisawa, J
AU  - Fujisawa J
FAU - Seiki, M
AU  - Seiki M
FAU - Sato, M
AU  - Sato M
FAU - Yoshida, M
AU  - Yoshida M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Viral Proteins)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - EC 3.1.21.- (DNA Restriction Enzymes)
SB  - IM
MH  - Acetyltransferases/genetics
MH  - Base Sequence
MH  - Chloramphenicol O-Acetyltransferase
MH  - Chromosome Deletion
MH  - DNA Restriction Enzymes
MH  - Deltaretrovirus/*genetics/growth & development
MH  - *Enhancer Elements, Genetic
MH  - *Genes
MH  - *Genes, Regulator
MH  - *Genes, Viral
MH  - Humans
MH  - Mutation
MH  - Plasmids
MH  - Promoter Regions, Genetic
MH  - Simian virus 40/genetics
MH  - *Transcription, Genetic
MH  - Transfection
MH  - Viral Proteins/*genetics
MH  - *Virus Activation
PMC - PMC1166849
EDAT- 1986/04/01 00:00
MHDA- 1986/04/01 00:01
PMCR- 1987/04/01
CRDT- 1986/04/01 00:00
PHST- 1986/04/01 00:00 [pubmed]
PHST- 1986/04/01 00:01 [medline]
PHST- 1986/04/01 00:00 [entrez]
PHST- 1987/04/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1986.tb04272.x [doi]
PST - ppublish
SO  - EMBO J. 1986 Apr;5(4):713-8. doi: 10.1002/j.1460-2075.1986.tb04272.x.